mRNA_D-dudresnayi_contig10717.788.1 (mRNA) Desmarestia dudresnayi DdudBR16 monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_D-dudresnayi_contig10717.788.1
Unique NamemRNA_D-dudresnayi_contig10717.788.1
TypemRNA
OrganismDesmarestia dudresnayi DdudBR16 monoicous (Desmarestia dudresnayi DdudBR16 monoicous)
Homology
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BLAST of mRNA_D-dudresnayi_contig10717.788.1 vs. uniprot
Match: D8LI35_ECTSI (Ribosome biogenesis protein WDR12 homolog n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LI35_ECTSI)

HSP 1 Score: 198 bits (503), Expect = 2.690e-59
Identity = 96/107 (89.72%), Postives = 105/107 (98.13%), Query Frame = 1 
Query:   52 MDFGDPSDDEADAGKVRVKFVTKIPSIRVTDTPIAVPETLQRYGLSEVVNHLLGLDPPTPFDMLVMGQFLRGSLRSVMQRNALSGESVLEVEYLPAVRQPTEAEHAD 372
            MDFG+PSDDEADAGKVRVKFVTK+PSIRVT+TP+AVPE+LQRYGLSEVVNHLLG DPPTPFDMLVMG+FLR SLRSVMQR+ALSGE+VLEVEYLPA+RQPTEAEHAD
Sbjct:    1 MDFGEPSDDEADAGKVRVKFVTKVPSIRVTETPVAVPESLQRYGLSEVVNHLLGRDPPTPFDMLVMGRFLRSSLRSVMQRHALSGEAVLEVEYLPALRQPTEAEHAD 107
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BLAST of mRNA_D-dudresnayi_contig10717.788.1 vs. uniprot
Match: A0A836CE58_9STRA (Ribosome biogenesis protein WDR12 homolog n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CE58_9STRA)

HSP 1 Score: 118 bits (296), Expect = 7.090e-29
Identity = 68/112 (60.71%), Postives = 80/112 (71.43%), Query Frame = 1 
Query:   94 KVRVKFVTKIPSIRVTDTPIAVPETLQRYGLSEVVNHLLGLDP-------------------PTPFDMLVMGQFLRGSLRSVMQRNALSGESVLEVEYLPAVRQPTEAEHAD 372
            +VRVKFVTK+ SIRVTD PIAVPETL RYGLSEVVNHLLGL+                    P PFD+L+ G+FLR SL   M+ +A+SGE+VLE+EYLPA+RQPTE E AD
Sbjct:   29 QVRVKFVTKLASIRVTDLPIAVPETLGRYGLSEVVNHLLGLNADTSAAAAAPHDAAPPSSKAPIPFDILIDGRFLRTSLARYMRAHAVSGETVLELEYLPALRQPTE-ESAD 139
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BLAST of mRNA_D-dudresnayi_contig10717.788.1 vs. uniprot
Match: A0A7S4DK03_HETAK (Hypothetical protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S4DK03_HETAK)

HSP 1 Score: 101 bits (252), Expect = 1.650e-25
Identity = 59/102 (57.84%), Postives = 72/102 (70.59%), Query Frame = 1 
Query:   70 SDDEADAGKVRVKFVTKIPSIRVTDTPIAVPETLQRYGLSEVVNHLLGLDPPTPFDMLVMGQFLRGSLRSVMQRN----ALSGESVLEVEYLPAVRQPTEAE 363
            SDDE +  KVRVKFVTKI SIRVT+ PIAVP +L RYGLSEVVNHLL  + P P + LV G   +G LR  ++R+     LSGE+V+E+EY PA   P +AE
Sbjct:    3 SDDETE--KVRVKFVTKIASIRVTEVPIAVPTSLGRYGLSEVVNHLLAREEPIPLEFLVEG---KGLLRVPLERHLTAAGLSGETVVELEYFPAAPPPEQAE 99
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BLAST of mRNA_D-dudresnayi_contig10717.788.1 vs. uniprot
Match: A0A7S2UZW3_9STRA (Hypothetical protein (Fragment) n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2UZW3_9STRA)

HSP 1 Score: 103 bits (257), Expect = 2.280e-25
Identity = 55/103 (53.40%), Postives = 70/103 (67.96%), Query Frame = 1 
Query:   73 DDEADAGKVRVKFVTKIPSIRVTDTPIAVPETLQRYGLSEVVNHLLGLDPPTPFDMLVM-----GQFLRGSLRSVMQRNALSGESVLEVEYLPAVRQPTEAEH 366
            +DE +  KVRV+FVTK+PS++VT+ PIAVP  L RYGLSEVVNHLLG++   PFD LV         LR  L+  MQ  ++SGESV+ +EY  A + P EA H
Sbjct:    3 NDEEEGEKVRVRFVTKVPSLKVTEMPIAVPIKLARYGLSEVVNHLLGVEKHVPFDFLVQDPRSRASLLRTPLKRHMQTWSISGESVVTLEYFEAAKPPQEAPH 105
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BLAST of mRNA_D-dudresnayi_contig10717.788.1 vs. uniprot
Match: A0A7S0KMA7_MICPS (Hypothetical protein (Fragment) n=1 Tax=Micromonas pusilla TaxID=38833 RepID=A0A7S0KMA7_MICPS)

HSP 1 Score: 105 bits (263), Expect = 9.620e-25
Identity = 52/89 (58.43%), Postives = 65/89 (73.03%), Query Frame = 1 
Query:   94 KVRVKFVTKIPS-IRVTDTPIAVPETLQRYGLSEVVNHLLGLDPPTPFDMLVMGQFLRGSLRSVMQRNALSGESVLEVEYLPAVRQPTE 357
            ++  KFVT++P   R+  TPIAVP  L RYGLSEV+NHLL LDPP PFD LV G+ +R SL  ++ R A+S ES LEVEY+PAV  P+E
Sbjct:    7 QISCKFVTRLPEQYRIAPTPIAVPGKLTRYGLSEVINHLLNLDPPKPFDFLVQGELVRTSLEKLVVRKAISAESALEVEYIPAVAPPSE 95
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BLAST of mRNA_D-dudresnayi_contig10717.788.1 vs. uniprot
Match: C1EJB9_MICCC (Ribosome biogenesis protein WDR12 homolog n=1 Tax=Micromonas commoda (strain RCC299 / NOUM17 / CCMP2709) TaxID=296587 RepID=C1EJB9_MICCC)

HSP 1 Score: 105 bits (262), Expect = 3.250e-24
Identity = 53/93 (56.99%), Postives = 67/93 (72.04%), Query Frame = 1 
Query:   82 ADAGKVRVKFVTKIPS-IRVTDTPIAVPETLQRYGLSEVVNHLLGLDPPTPFDMLVMGQFLRGSLRSVMQRNALSGESVLEVEYLPAVRQPTE 357
            A+  ++  KFVT++P   R+  TPIAVP  L RYGLSEV+NHLL LDPP PFD LV G+ +R SL  ++ R A+S ES LEVEY+PAV  P+E
Sbjct:    3 AEEVQISCKFVTRLPEQYRIAPTPIAVPGKLTRYGLSEVINHLLNLDPPKPFDFLVQGELVRTSLEKLVVRKAISVESALEVEYIPAVAPPSE 95
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BLAST of mRNA_D-dudresnayi_contig10717.788.1 vs. uniprot
Match: A0A2J7ZLP8_9CHLO (Ribosome biogenesis protein WDR12 homolog n=1 Tax=Tetrabaena socialis TaxID=47790 RepID=A0A2J7ZLP8_9CHLO)

HSP 1 Score: 104 bits (260), Expect = 6.470e-24
Identity = 52/91 (57.14%), Postives = 70/91 (76.92%), Query Frame = 1 
Query:   82 ADAGKVRVKFVTKIP-SIRVTDTPIAVPETLQRYGLSEVVNHLLGLDPPTPFDMLVMGQFLRGSLRSVMQRNALSGESVLEVEYLPAVRQP 351
            A+  +V VKFVTK+P S+RV +TP+AVP TL+RYGLS+++NHLL LDPP PFD LV G+ +R SL + +  + LS ES L++EY+PAV  P
Sbjct:    2 AEETQVLVKFVTKLPISLRVPETPVAVPSTLRRYGLSQIINHLLALDPPRPFDFLVNGELVRCSLEAHLLGHNLSAESTLQLEYVPAVVPP 92
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BLAST of mRNA_D-dudresnayi_contig10717.788.1 vs. uniprot
Match: A0A836BU45_9CHLO (Ribosome biogenesis protein WDR12 homolog n=1 Tax=Edaphochlamys debaryana TaxID=47281 RepID=A0A836BU45_9CHLO)

HSP 1 Score: 104 bits (259), Expect = 9.930e-24
Identity = 53/91 (58.24%), Postives = 67/91 (73.63%), Query Frame = 1 
Query:   82 ADAGKVRVKFVTKIPS-IRVTDTPIAVPETLQRYGLSEVVNHLLGLDPPTPFDMLVMGQFLRGSLRSVMQRNALSGESVLEVEYLPAVRQP 351
            A+  +V VKF+TK+P  +RV +TP+AVP +L+RYGLS+V+NHLL LD P PFD LV GQ LR SL + +    LS ES LEVEY+PAV  P
Sbjct:    3 AEESQVLVKFITKLPPHLRVPETPVAVPASLRRYGLSQVINHLLALDAPRPFDFLVSGQLLRSSLEAHLLLAGLSAESTLEVEYVPAVVPP 93
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BLAST of mRNA_D-dudresnayi_contig10717.788.1 vs. uniprot
Match: A0A7S0GRJ0_MICPS (Hypothetical protein (Fragment) n=1 Tax=Micromonas pusilla TaxID=38833 RepID=A0A7S0GRJ0_MICPS)

HSP 1 Score: 101 bits (251), Expect = 1.830e-23
Identity = 49/89 (55.06%), Postives = 65/89 (73.03%), Query Frame = 1 
Query:   94 KVRVKFVTKIPS-IRVTDTPIAVPETLQRYGLSEVVNHLLGLDPPTPFDMLVMGQFLRGSLRSVMQRNALSGESVLEVEYLPAVRQPTE 357
            ++  +FVT++P   R+  TP+AVP  L RYGLSEVVNHLL LDPP PFD LV G+ +R SL+ ++ R A+S E  L+VEY+PAV  P+E
Sbjct:    9 QITCRFVTRLPEKFRIAPTPLAVPGKLTRYGLSEVVNHLLALDPPKPFDFLVDGELVRTSLQKLLLRKAISAEKTLDVEYIPAVGPPSE 97
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BLAST of mRNA_D-dudresnayi_contig10717.788.1 vs. uniprot
Match: WDR12_CHLRE (Ribosome biogenesis protein WDR12 homolog n=4 Tax=Chlamydomonas TaxID=3052 RepID=WDR12_CHLRE)

HSP 1 Score: 103 bits (256), Expect = 2.050e-23
Identity = 51/91 (56.04%), Postives = 68/91 (74.73%), Query Frame = 1 
Query:   82 ADAGKVRVKFVTKIPS-IRVTDTPIAVPETLQRYGLSEVVNHLLGLDPPTPFDMLVMGQFLRGSLRSVMQRNALSGESVLEVEYLPAVRQP 351
            A+  +V VKFVTK+P+ +RV +TP+AVP  L+RYGLS+++NHLL LDPP PFD L+ G+ +R SL   +  + LS ES LEVEY+PAV  P
Sbjct:    2 AEETQVLVKFVTKLPAHLRVPETPVAVPSDLKRYGLSQIINHLLALDPPRPFDFLIDGELVRKSLEQHLLAHNLSAESTLEVEYVPAVVPP 92
The following BLAST results are available for this feature:
BLAST of mRNA_D-dudresnayi_contig10717.788.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LI35_ECTSI2.690e-5989.72Ribosome biogenesis protein WDR12 homolog n=1 Tax=... [more]
A0A836CE58_9STRA7.090e-2960.71Ribosome biogenesis protein WDR12 homolog n=1 Tax=... [more]
A0A7S4DK03_HETAK1.650e-2557.84Hypothetical protein (Fragment) n=1 Tax=Heterosigm... [more]
A0A7S2UZW3_9STRA2.280e-2553.40Hypothetical protein (Fragment) n=1 Tax=Fibrocapsa... [more]
A0A7S0KMA7_MICPS9.620e-2558.43Hypothetical protein (Fragment) n=1 Tax=Micromonas... [more]
C1EJB9_MICCC3.250e-2456.99Ribosome biogenesis protein WDR12 homolog n=1 Tax=... [more]
A0A2J7ZLP8_9CHLO6.470e-2457.14Ribosome biogenesis protein WDR12 homolog n=1 Tax=... [more]
A0A836BU45_9CHLO9.930e-2458.24Ribosome biogenesis protein WDR12 homolog n=1 Tax=... [more]
A0A7S0GRJ0_MICPS1.830e-2355.06Hypothetical protein (Fragment) n=1 Tax=Micromonas... [more]
WDR12_CHLRE2.050e-2356.04Ribosome biogenesis protein WDR12 homolog n=4 Tax=... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
D-dudresnayi_contig10717contigD-dudresnayi_contig10717:211..1788 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
OGS1.0 of Desmarestia dudresnayi DdudBR16 monoicous2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score196.8
Seed ortholog evalue5.1e-48
Seed eggNOG ortholog2880.D8LI35
Preferred nameYTM1
KEGG koko:K11578,ko:K14863
GOsGO:0000151,GO:0000460,GO:0000470,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005730,GO:0005911,GO:0006139,GO:0006364,GO:0006396,GO:0006725,GO:0006807,GO:0006950,GO:0006996,GO:0007154,GO:0008150,GO:0008152,GO:0009267,GO:0009506,GO:0009605,GO:0009607,GO:0009987,GO:0009991,GO:0010035,GO:0010038,GO:0010467,GO:0016043,GO:0016070,GO:0016072,GO:0022613,GO:0030054,GO:0030447,GO:0030684,GO:0030686,GO:0030687,GO:0031461,GO:0031667,GO:0031668,GO:0031669,GO:0031974,GO:0031981,GO:0032991,GO:0033554,GO:0034470,GO:0034641,GO:0034660,GO:0036170,GO:0036180,GO:0040007,GO:0042221,GO:0042254,GO:0042273,GO:0042594,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0044085,GO:0044182,GO:0044237,GO:0044238,GO:0044422,GO:0044424,GO:0044428,GO:0044446,GO:0044452,GO:0044464,GO:0046483,GO:0046689,GO:0050896,GO:0051276,GO:0051716,GO:0055044,GO:0070013,GO:0070545,GO:0071496,GO:0071704,GO:0071840,GO:0080008,GO:0090304,GO:1901360,GO:1902494,GO:1990234,GO:1990904
EggNOG free text desc.ribosomal large subunit biogenesis
EggNOG OGsKOG0313@1,KOG0313@2759
Ec32 ortholog descriptionWD40-repeat-containing domain
Ec32 orthologEc-04_003080.1
COG Functional cat.J
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03009,ko03036,ko04131
Hectar predicted targeting categoryother localisation
Exons2
Model size372
Cds size321
Stop0
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_D-dudresnayi_contig10717.788.1prot_D-dudresnayi_contig10717.788.1Desmarestia dudresnayi DdudBR16 monoicouspolypeptideD-dudresnayi_contig10717 211..1737 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927581.0388966-CDS-D-dudresnayi_contig10717:210..4861622927581.0388966-CDS-D-dudresnayi_contig10717:210..486Desmarestia dudresnayi DdudBR16 monoicousCDSD-dudresnayi_contig10717 211..486 -
1690556815.50023-CDS-D-dudresnayi_contig10717:210..4861690556815.50023-CDS-D-dudresnayi_contig10717:210..486Desmarestia dudresnayi DdudBR16 monoicousCDSD-dudresnayi_contig10717 211..486 -
1622927581.053124-CDS-D-dudresnayi_contig10717:1692..17371622927581.053124-CDS-D-dudresnayi_contig10717:1692..1737Desmarestia dudresnayi DdudBR16 monoicousCDSD-dudresnayi_contig10717 1693..1737 -
1690556815.5112681-CDS-D-dudresnayi_contig10717:1692..17371690556815.5112681-CDS-D-dudresnayi_contig10717:1692..1737Desmarestia dudresnayi DdudBR16 monoicousCDSD-dudresnayi_contig10717 1693..1737 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622927581.067782-UTR-D-dudresnayi_contig10717:1737..17881622927581.067782-UTR-D-dudresnayi_contig10717:1737..1788Desmarestia dudresnayi DdudBR16 monoicousUTRD-dudresnayi_contig10717 1738..1788 -
1690556815.5214243-UTR-D-dudresnayi_contig10717:1737..17881690556815.5214243-UTR-D-dudresnayi_contig10717:1737..1788Desmarestia dudresnayi DdudBR16 monoicousUTRD-dudresnayi_contig10717 1738..1788 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_D-dudresnayi_contig10717.788.1

>prot_D-dudresnayi_contig10717.788.1 ID=prot_D-dudresnayi_contig10717.788.1|Name=mRNA_D-dudresnayi_contig10717.788.1|organism=Desmarestia dudresnayi DdudBR16 monoicous|type=polypeptide|length=107bp
MDFGDPSDDEADAGKVRVKFVTKIPSIRVTDTPIAVPETLQRYGLSEVVN
HLLGLDPPTPFDMLVMGQFLRGSLRSVMQRNALSGESVLEVEYLPAVRQP
TEAEHAD
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mRNA from alignment at D-dudresnayi_contig10717:211..1788-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_D-dudresnayi_contig10717.788.1 ID=mRNA_D-dudresnayi_contig10717.788.1|Name=mRNA_D-dudresnayi_contig10717.788.1|organism=Desmarestia dudresnayi DdudBR16 monoicous|type=mRNA|length=1578bp|location=Sequence derived from alignment at D-dudresnayi_contig10717:211..1788- (Desmarestia dudresnayi DdudBR16 monoicous)
CGACGGTAGTGCTCGAACTCTGGTGTGGTACGGGGCGGCCTGTTTTGTAC
CATGGACTTCGGTGACCCTTCCGATGACGAGGCCGATGCTGGAAAGGTGA
GAATGTAGAAGGCTTGTAGGGACGCTGGCAGCAGGGAGAGCACTACCCAC
CAGCGAGAATGATCGCTGTCCAGCAGTCACCAATGTCGCAGATAGGATAC
AGAATATAGAAGGTTTCTGCGGCTGAGGCCGCAACGCTCAAATAACGGGA
GCGAGTCGCCAAGGCCAAAGGACATAGTAGCCGCAATAGGTTGATGGCGT
TGAGAATAGGGTTGTTGTGATAATTGGGACGCTCATTGCATTCATTGGTC
ACAGAACCGTCGTATGATCTGTGATACGAGACAATCATATCCCATGGCAA
GAATGATGGCAAGCATGGAGGTTGCCAGTTGGTCGTATCAGAACGGGGTG
GGGGGGGGTATGACAGCAATAACACAGGCAGGCAGCAGCAGGAAAACATA
CACTTTAGATATAATATTTGAGGTACCACGGTACCATACACCTGTCATAC
GTACACGCTTCAGACAACATCAACATTTACAGGGCCATAGGGCTTAGCGG
TGTGACCGTTAGCGTGCTGTCTAGAGACGAAAGACGGGTGTTCTATTAAT
AGTTATATGACAGAACCGCCACGATTGGAGGTGATAGGAGCAGTAGTAAC
AACGATGCTGCGACACTGCAAGTATCTTGATAAGGGTGTTGCCTAGACGG
TATATACTCGTAGGGCTTGACAGCAGGGCCTTGGGTAGTCGTCTCGCCTT
TGGTCTTCGACGTACGCTTACCAGGTCGCTCATGGCGTGCATGGTGGGTA
TGATACAGAGTGTCGGAATCATGTGTTCACTATGTCTACTGACTATACTT
TGAAGACGTTGGGCATACAATGGGACCATACTACCTACCACGTAATGCGT
GGGCAGTGGCCAGGATGTAAGAGATTGAACCCGGTGCGCTTCTGGCCCCA
TCTATTAGTAAGAGGTGCTCACATCTCCCACAGACACATGACACAGACGT
TGTGCTGTGCGTTTGCTTGTCGTGTGTTACAAAGCTGAAGATTTCATATA
GAGCCACATGGGTCCGCGAATATCTACAGTAGTTTAAAAGCACCACTACT
GTTGTGACATCATACAAGTAGGTTAAAGGCTGCCGTGTGGGCTTGGTAAA
CACGAAGTCATTCACGACTGCTCCCGTTCTACCGTCGCTTGTTTGATTGC
CCTACCCTTTGTCTTACAACAAACACACGCGAACACACATACATAAACTC
AGGTACGGGTAAAGTTCGTGACCAAGATTCCCTCCATCCGGGTGACGGAT
ACGCCGATAGCCGTGCCGGAGACGCTCCAGCGCTATGGCTTGTCCGAGGT
GGTCAACCACCTCCTGGGGCTCGACCCACCCACGCCGTTCGACATGCTGG
TCATGGGGCAATTCCTTCGCGGCTCTCTGAGGTCGGTGATGCAGCGGAAC
GCGTTGAGCGGCGAGTCTGTGCTGGAGGTGGAGTATTTGCCGGCTGTACG
CCAGCCAACGGAGGCCGAGCACGCAGAC
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Coding sequence (CDS) from alignment at D-dudresnayi_contig10717:211..1788-

>mRNA_D-dudresnayi_contig10717.788.1 ID=mRNA_D-dudresnayi_contig10717.788.1|Name=mRNA_D-dudresnayi_contig10717.788.1|organism=Desmarestia dudresnayi DdudBR16 monoicous|type=CDS|length=642bp|location=Sequence derived from alignment at D-dudresnayi_contig10717:211..1788- (Desmarestia dudresnayi DdudBR16 monoicous)
ATGGACTTCGGTGACCCTTCCGATGACGAGGCCGATGCTGGAAAGATGGA
CTTCGGTGACCCTTCCGATGACGAGGCCGATGCTGGAAAGGTACGGGTAA
AGTTCGTGACCAAGATTCCCTCCATCCGGGTGACGGATACGCCGATAGCC
GTGCCGGAGACGCTCCAGCGCTATGGCTTGTCCGAGGTGGTCAACCACCT
CCTGGGGCTCGACCCACCCACGCCGTTCGACATGCTGGTCATGGGGCAAT
TCCTTCGCGGCTCTCTGAGGTCGGTGATGCAGCGGAACGCGTTGAGCGGC
GAGTCTGTGCTGGAGGTGGAGTATTTGCCGGCTGTACGCCAGCCAACGGA
GGCCGAGCACGCAGACGTACGGGTAAAGTTCGTGACCAAGATTCCCTCCA
TCCGGGTGACGGATACGCCGATAGCCGTGCCGGAGACGCTCCAGCGCTAT
GGCTTGTCCGAGGTGGTCAACCACCTCCTGGGGCTCGACCCACCCACGCC
GTTCGACATGCTGGTCATGGGGCAATTCCTTCGCGGCTCTCTGAGGTCGG
TGATGCAGCGGAACGCGTTGAGCGGCGAGTCTGTGCTGGAGGTGGAGTAT
TTGCCGGCTGTACGCCAGCCAACGGAGGCCGAGCACGCAGAC
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