mRNA_C-tenellus_contig8407.5.1 (mRNA) Choristocarpus tenellus KU2346

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_C-tenellus_contig8407.5.1
Unique NamemRNA_C-tenellus_contig8407.5.1
TypemRNA
OrganismChoristocarpus tenellus KU2346 (Choristocarpus tenellus KU2346)
Homology
BLAST of mRNA_C-tenellus_contig8407.5.1 vs. uniprot
Match: D7FXJ9_ECTSI (PAP_fibrillin domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FXJ9_ECTSI)

HSP 1 Score: 198 bits (503), Expect = 1.430e-59
Identity = 108/220 (49.09%), Postives = 137/220 (62.27%), Query Frame = 1
Query:    1 GEDFVGTNRTEAGLECTLGRLAFNIFEPIDLPILITSIGNKVGTEEIHTSATESSTASPSGERRGDAPSAGSLASAGTHTYSICTNFVTRDEKALRGRLILEGSCEEHPKRSDRLNVRFLSGYIEPDDGQDLDAWCAVIGQRDGASKK------RLKARAVELMLKLVFGYRPPRGGLGEHGEMSYTMTRPPKGSLDILYLDEELRVTRGQRGSVIIVER 642
            GEDF GT RT+ G+ECTLGRL FNIFEP++LP+LI +I N +G    +        A P+ E                  Y I T +V RD   LRGR+ + G+C E P   +R+ VRF  G IEPD+GQDLDAW  VIG RD    K       LK +A  LMLK+VFG++ P  GLG  GE+SY M R PKG LDI+YLDEE+RVTRGQ+G++++V+R
Sbjct:  107 GEDFKGTTRTKDGVECTLGRLVFNIFEPVNLPVLIVAIKNTIGDRPEN--------ADPAYESN----------------YVISTRWVARDGSGLRGRVEISGTCWEDPSMPERVLVRFTKGSIEPDEGQDLDAWRKVIGVRDVEGTKDKGPLSTLKEKAARLMLKVVFGFQGPADGLGPRGELSYEMKRSPKGYLDIIYLDEEMRVTRGQQGALVVVDR 302          
BLAST of mRNA_C-tenellus_contig8407.5.1 vs. uniprot
Match: A0A835ZKX0_9STRA (PAP_fibrillin domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZKX0_9STRA)

HSP 1 Score: 149 bits (377), Expect = 1.220e-40
Identity = 94/219 (42.92%), Postives = 120/219 (54.79%), Query Frame = 1
Query:    1 GEDFVGTNRTEAGLECTLGRLAFNIFEPIDLPILITSIGNKVGTEEIHTSATESSTASPSGERRGDAPSAGSLASAGTHTYSICTNFVTRDEKA---LRGRLILEGSCEEHPKRSDRLNVRFLSGYIEPDDGQDLDAWCAVIGQRDGASKKR--LKARAVELMLKLVFGYRPPRGGLGEHGEMSYTMTRPPKGSLDILYLDEELRVTRGQRGSVIIVER 642
            G +F    R   G ECTLGRLAFN+FEP  LP+ IT I N +  E++              ER+GD P        G  TY +  +FV R  ++   LRG + + G C    K ++RL V F +G + P  GQDL  W AV   R+G  K R  L  +   LMLKL  G++PP       G   YTM RPP+G LDILYLD+ LRVTRG RGS+++VER
Sbjct:  112 GFNFPDGKRGPLGWECTLGRLAFNLFEPTKLPVSITHIVNTL--EDVPA------------ERQGDRP--------GELTYDLVNSFVARSPESGVELRGEVAVSGVCWPDSKDANRLRVAFGAGELRPAQGQDLAEWEAVFKPREGKEKPRVPLGKKLTNLMLKLFMGFQPPADA--GQGVQRYTMNRPPEGYLDILYLDDALRVTRGNRGSIVVVER 306          
BLAST of mRNA_C-tenellus_contig8407.5.1 vs. uniprot
Match: A0A6H2NGX9_9CYAN (Fimbrial protein n=1 Tax=Leptolyngbya sp. LCM1.Bin17 TaxID=2480211 RepID=A0A6H2NGX9_9CYAN)

HSP 1 Score: 120 bits (301), Expect = 7.020e-30
Identity = 73/199 (36.68%), Postives = 103/199 (51.76%), Query Frame = 1
Query:   49 TLGRLAFNIFEPIDLPILITSIGNKVGTEEIHTSATESSTASPSGERRGDAPSAGSLASAGTHTYSICTNFVTRD-EKALRGRLILEGSCEEHPKRSDRLNVRFLSGYIEPDDGQDLDAWCAVIGQRDGASKKRLKARAVELMLKLVFGYRPPRGGLGEHGEMSYTMTRPPKGSLDILYLDEELRVTRGQRGSVIIVER 642
            TLGRLAFN+F+P D+ ++I  +   V                              +A    HT+ I  +F T D E  L+GR+   G CE  P  + +L V+F  G +EP    D D W  V G  D A +  L AR   L+LKL+FG  PP     + G + + M R PKG+L +LYLDE+LR+TRG++G+V+I +R
Sbjct:   86 TLGRLAFNMFQPQDMKLVINQVSQPVWP----------------------------IADGPQHTHDIVVDFTTLDLEVPLQGRVRNLGICE--PATASQLQVQFTGGVLEPAADSDRDHWHQVFGDPDAAPRLGLTARLQGLVLKLMFGLVPPTPMAPDTGRIEFQMRRSPKGTLTVLYLDEDLRITRGEKGTVLICDR 254          
BLAST of mRNA_C-tenellus_contig8407.5.1 vs. uniprot
Match: UPI00146A6814 (fimbrial protein n=1 Tax=Nodosilinea sp. P-1105 TaxID=2546229 RepID=UPI00146A6814)

HSP 1 Score: 120 bits (301), Expect = 7.020e-30
Identity = 70/199 (35.18%), Postives = 105/199 (52.76%), Query Frame = 1
Query:   49 TLGRLAFNIFEPIDLPILITSIGNKVGTEEIHTSATESSTASPSGERRGDAPSAGSLASAGTHTYSICTNFVTRD-EKALRGRLILEGSCEEHPKRSDRLNVRFLSGYIEPDDGQDLDAWCAVIGQRDGASKKRLKARAVELMLKLVFGYRPPRGGLGEHGEMSYTMTRPPKGSLDILYLDEELRVTRGQRGSVIIVER 642
            TLGRLAFN+F+P  L ++I  +   V                              +A    HT+ I  +F T D E  L+GR+   G C+  P  +++L V+F  G +EP    D D W  V G  D + +  LKAR   L+LKL+FG  PP+    + G + + M R PKG L+ILYLD++LR+TRG++G++++ +R
Sbjct:   86 TLGRLAFNMFQPQTLKLVIDQVSQPVWP----------------------------IADGAHHTHDIVVDFTTLDLEVPLQGRVRNLGICQ--PATANQLQVQFTGGVLEPAVDSDRDHWHQVFGDPDASPRPGLKARLQGLVLKLMFGLVPPKAMAADTGRIEFQMRRSPKGRLEILYLDDDLRITRGEKGTILVCDR 254          
BLAST of mRNA_C-tenellus_contig8407.5.1 vs. uniprot
Match: A0A846BZ97_9CYAN (Fimbrial protein n=4 Tax=unclassified Okeania TaxID=2634635 RepID=A0A846BZ97_9CYAN)

HSP 1 Score: 118 bits (296), Expect = 3.680e-29
Identity = 75/200 (37.50%), Postives = 107/200 (53.50%), Query Frame = 1
Query:   49 TLGRLAFNIFEPIDLPILITSIGNKVGTEEIHTSATESSTASPSGERRGDAPSAGSLASAGTHTYSICTNFVTRDE--KALRGRLILEGSCEEHPKRSDRLNVRFLSGYIEPDDGQDLDAWCAVIGQRDGASKKRLKARAVELMLKLVFGYRPPRGGLGEHGEMSYTMTRPPKGSLDILYLDEELRVTRGQRGSVIIVER 642
            TLGRLAFN+FEP +L ++I S+   V                  GE+R               ++ I   F   DE    L+G +   G C  +P  +  L V F  G + P  G+DL  W  + GQ   +SKK LK + + + LKL+FG  PP+G   E GE+S+TM R PKG L++LYLDEE R+T+G++G+V++ ER
Sbjct:   79 TLGRLAFNMFEPTNLKLVIDSVRQPV-------------VLLGEGEKR---------------SHDIVVEFSIIDELFPPLKGIVRNLGVC--YPADNQNLQVEFTGGTLGPQKGEDLSTWKDIFGQNK-SSKKGLKEQLMSVFLKLMFGLVPPQGMNTETGEISFTMKRSPKGRLEVLYLDEEFRITKGEKGTVLVCER 247          
BLAST of mRNA_C-tenellus_contig8407.5.1 vs. uniprot
Match: A0A846FND7_9CYAN (Fimbrial protein n=1 Tax=Cyanothece sp. SIO1E1 TaxID=2607788 RepID=A0A846FND7_9CYAN)

HSP 1 Score: 117 bits (293), Expect = 9.330e-29
Identity = 72/200 (36.00%), Postives = 107/200 (53.50%), Query Frame = 1
Query:   49 TLGRLAFNIFEPIDLPILITSIGNKVGTEEIHTSATESSTASPSGERRGDAPSAGSLASAGTHTYSICTNFVTRDEK--ALRGRLILEGSCEEHPKRSDRLNVRFLSGYIEPDDGQDLDAWCAVIGQRDGASKKRLKARAVELMLKLVFGYRPPRGGLGEHGEMSYTMTRPPKGSLDILYLDEELRVTRGQRGSVIIVER 642
            TLGRLAFN+F+P  L ++I  +   V               + +G++R               T+ I T F T +E    L+G +   G CE  P   D L V+F  G + P D  +L+AW A+ G +   +KK LK +     L ++FG  PP+G   + G++S+ M R PKG L +LYLDEELR+T+G+RG+V++ ER
Sbjct:   80 TLGRLAFNMFQPTHLQVVIDRVLQPV-------------VPTGNGQQR---------------THDIITEFTTVNENIPTLQGIVYNFGVCE--PADDDALQVQFTGGALAPKDTHNLEAWKAIFGDQSKPAKKSLKEKLSSAFLGIMFGLVPPQGMEPDTGQISFKMRRSPKGKLKLLYLDEELRITQGERGTVLVCER 249          
BLAST of mRNA_C-tenellus_contig8407.5.1 vs. uniprot
Match: UPI001785C01E (fimbrial protein n=1 Tax=Microcoleus sp. FACHB-SPT15 TaxID=2692830 RepID=UPI001785C01E)

HSP 1 Score: 117 bits (292), Expect = 1.370e-28
Identity = 74/211 (35.07%), Postives = 109/211 (51.66%), Query Frame = 1
Query:   16 GTNRTEAGLECTLGRLAFNIFEPIDLPILITSIGNKVGTEEIHTSATESSTASPSGERRGDAPSAGSLASAGTHTYSICTNFVTRDEKA--LRGRLILEGSCEEHPKRSDRLNVRFLSGYIEPDDGQDLDAWCAVIGQRDGASKKRLKARAVELMLKLVFGYRPPRGGLGEHGEMSYTMTRPPKGSLDILYLDEELRVTRGQRGSVIIVER 642
            G  R++     TLGRLAFN+F+P  L I+I  +   V                P GE +               T+ I   F T DE    L+G +  +G C  H   +  L V+F  G + P    +++AW AV G++   S   LK R +  +L+++FG  PP+G     G +S+ M R PKG+L++LYLDEELR+TRG+RG+V++ ER
Sbjct:   69 GELRSDGKYVYTLGRLAFNLFQPTSLKIVIDRVLQPVV---------------PVGEGQ-------------QRTHDIIVEFTTVDENTPTLQGIVHNQGICSPHDDNT--LQVQFTGGELAPKAPNEIEAWKAVFGEQSKPSTTGLKERLMTSVLRMMFGIVPPQGMDRNTGRVSFKMRRSPKGNLELLYLDEELRITRGERGTVLVCER 249          
BLAST of mRNA_C-tenellus_contig8407.5.1 vs. uniprot
Match: A0A1Z4N4X8_9CYAN (PAP_fibrillin domain-containing protein n=5 Tax=Nostocales TaxID=1161 RepID=A0A1Z4N4X8_9CYAN)

HSP 1 Score: 114 bits (284), Expect = 2.000e-27
Identity = 71/215 (33.02%), Postives = 108/215 (50.23%), Query Frame = 1
Query:    7 DFVGTNRTEAGLEC-TLGRLAFNIFEPIDLPILITSIGNKVGTEEIHTSATESSTASPSGERRGDAPSAGSLASAGTHTYSICTNFVTRDEKA--LRGRLILEGSCEEHPKRSDRLNVRFLSGYIEPDDGQDLDAWCAVIGQRDGASKKRLKARAVELMLKLVFGYRPPRGGLGEHGEMSYTMTRPPKGSLDILYLDEELRVTRGQRGSVIIVER 642
            +F G  +   G  C TLGRLAFN+F+P  L + +  +   V                              L +   H++ I   F T DE    L G +  +G C+     +  L V F  G ++P D Q+L+ W AV G +  ++++  K   +   L L+FG  PP+G   + GE+S+TM R PKG L+ILY+DEELR+TRG+R ++++ ER
Sbjct:   65 NFPGGEQLADGSFCYTLGRLAFNMFQPTGLKVTLQRVSQPVFL----------------------------LGNGEQHSHDIVVEFTTIDESVPKLSGIVRNQGVCQVVSDTT--LQVEFTGGILQPQDNQNLEEWKAVFGNQQPSARRSFKDILMSGFLNLMFGLVPPQGMDADTGEISFTMRRSPKGRLEILYVDEELRITRGEREAILVSER 249          
BLAST of mRNA_C-tenellus_contig8407.5.1 vs. uniprot
Match: K9QSZ7_NOSS7 (PAP_fibrillin n=2 Tax=Nostocaceae TaxID=1162 RepID=K9QSZ7_NOSS7)

HSP 1 Score: 114 bits (284), Expect = 2.000e-27
Identity = 72/200 (36.00%), Postives = 106/200 (53.00%), Query Frame = 1
Query:   49 TLGRLAFNIFEPIDLPILITSIGNKVGTEEIHTSATESSTASPSGERRGDAPSAGSLASAGTHTYSICTNFVTRDEK--ALRGRLILEGSCEEHPKRSDRLNVRFLSGYIEPDDGQDLDAWCAVIGQRDGASKKRLKARAVELMLKLVFGYRPPRGGLGEHGEMSYTMTRPPKGSLDILYLDEELRVTRGQRGSVIIVER 642
            +LGRLAFN+F+P  L ++I  +   V                 +GE+R               T+ I   F T DE    L G +   G C+    R   L V+F  G + P D   +D W AV GQ+   +K+ +K   + ++L+L+FG  PP+G     GE+++TM R PKG L+ILYLDEELR+TRG++G+V++ ER
Sbjct:   80 SLGRLAFNMFQPTGLKLVIDRVLQPVFPVG-------------NGEQR---------------THDIVVEFTTIDESFPQLSGIVRNLGVCQPISDRV--LQVKFTGGVLAPQDPNSIDDWRAVFGQQSKPTKQTIKENLMSVVLRLMFGLVPPQGINPTTGEVAFTMERSPKGRLEILYLDEELRITRGEKGTVLVCER 249          
BLAST of mRNA_C-tenellus_contig8407.5.1 vs. uniprot
Match: UPI00082A7F03 (fimbrial protein n=2 Tax=unclassified Anabaena TaxID=2619674 RepID=UPI00082A7F03)

HSP 1 Score: 114 bits (284), Expect = 2.000e-27
Identity = 72/200 (36.00%), Postives = 103/200 (51.50%), Query Frame = 1
Query:   49 TLGRLAFNIFEPIDLPILITSIGNKVGTEEIHTSATESSTASPSGERRGDAPSAGSLASAGTHTYSICTNFVTRDEKA--LRGRLILEGSCEEHPKRSDRLNVRFLSGYIEPDDGQDLDAWCAVIGQRDGASKKRLKARAVELMLKLVFGYRPPRGGLGEHGEMSYTMTRPPKGSLDILYLDEELRVTRGQRGSVIIVER 642
            TLGRLAFN+FEP  L ++I  +   V                 +GE+R               T+ I   F T DE +  L G +   G C+  P     L V+F  G + P +   +DAW  V GQ+    ++  K   + + L+L+FG  PP+G     GE+++TM R PKG L ILYLDEELR+TRG++G+V++ ER
Sbjct:   80 TLGRLAFNMFEPTKLKLVIDRVLQPVLPVG-------------NGEQR---------------THDIVVEFTTIDESSAPLSGVVRNLGVCQ--PASDTVLQVKFTGGVLAPQNLNQIDAWRGVFGQQSQPKQRTFKEHLMSVFLRLMFGLVPPQGMNNTTGEVAFTMERSPKGRLSILYLDEELRITRGEKGTVLVCER 249          
The following BLAST results are available for this feature:
BLAST of mRNA_C-tenellus_contig8407.5.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FXJ9_ECTSI1.430e-5949.09PAP_fibrillin domain-containing protein n=2 Tax=Ec... [more]
A0A835ZKX0_9STRA1.220e-4042.92PAP_fibrillin domain-containing protein n=1 Tax=Tr... [more]
A0A6H2NGX9_9CYAN7.020e-3036.68Fimbrial protein n=1 Tax=Leptolyngbya sp. LCM1.Bin... [more]
UPI00146A68147.020e-3035.18fimbrial protein n=1 Tax=Nodosilinea sp. P-1105 Ta... [more]
A0A846BZ97_9CYAN3.680e-2937.50Fimbrial protein n=4 Tax=unclassified Okeania TaxI... [more]
A0A846FND7_9CYAN9.330e-2936.00Fimbrial protein n=1 Tax=Cyanothece sp. SIO1E1 Tax... [more]
UPI001785C01E1.370e-2835.07fimbrial protein n=1 Tax=Microcoleus sp. FACHB-SPT... [more]
A0A1Z4N4X8_9CYAN2.000e-2733.02PAP_fibrillin domain-containing protein n=5 Tax=No... [more]
K9QSZ7_NOSS72.000e-2736.00PAP_fibrillin n=2 Tax=Nostocaceae TaxID=1162 RepID... [more]
UPI00082A7F032.000e-2736.00fimbrial protein n=2 Tax=unclassified Anabaena Tax... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
C-tenellus_contig8407contigC-tenellus_contig8407:2583..3230 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Choristocarpus tenellus KU2346 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start0
Seed ortholog2880.D7FXJ9
PFAMsPAP_fibrillin
Model size648
Max annot lvl2759|Eukaryota
Hectar predicted targeting categoryother localisation
Exons1
Evalue2.35e-60
EggNOG OGs28N1U@1|root,2S7IC@2759|Eukaryota
Ec32 ortholog descriptionPlastid lipid-associated protein/fibrillin conserved domain
Ec32 orthologEc-28_001980.1
DescriptionPAP_fibrillin
Cds size648
COG categoryS
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680790103.6445725-CDS-C-tenellus_contig8407:2582..32301680790103.6445725-CDS-C-tenellus_contig8407:2582..3230Choristocarpus tenellus KU2346CDSC-tenellus_contig8407 2583..3230 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_C-tenellus_contig8407.5.1prot_C-tenellus_contig8407.5.1Choristocarpus tenellus KU2346polypeptideC-tenellus_contig8407 2583..3230 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_C-tenellus_contig8407.5.1

>prot_C-tenellus_contig8407.5.1 ID=prot_C-tenellus_contig8407.5.1|Name=mRNA_C-tenellus_contig8407.5.1|organism=Choristocarpus tenellus KU2346|type=polypeptide|length=216bp
GEDFVGTNRTEAGLECTLGRLAFNIFEPIDLPILITSIGNKVGTEEIHTS
ATESSTASPSGERRGDAPSAGSLASAGTHTYSICTNFVTRDEKALRGRLI
LEGSCEEHPKRSDRLNVRFLSGYIEPDDGQDLDAWCAVIGQRDGASKKRL
KARAVELMLKLVFGYRPPRGGLGEHGEMSYTMTRPPKGSLDILYLDEELR
VTRGQRGSVIIVERA*
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mRNA from alignment at C-tenellus_contig8407:2583..3230+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_C-tenellus_contig8407.5.1 ID=mRNA_C-tenellus_contig8407.5.1|Name=mRNA_C-tenellus_contig8407.5.1|organism=Choristocarpus tenellus KU2346|type=mRNA|length=648bp|location=Sequence derived from alignment at C-tenellus_contig8407:2583..3230+ (Choristocarpus tenellus KU2346)
GGAGAAGATTTTGTTGGCACAAATCGAACGGAAGCAGGGTTGGAATGCAC ACTAGGTCGGCTTGCTTTCAACATCTTTGAGCCCATTGACCTGCCAATTT TGATCACTTCCATTGGCAACAAAGTCGGGACAGAGGAAATTCATACTTCT GCCACAGAATCATCCACTGCCTCTCCTTCTGGGGAAAGGCGTGGAGATGC GCCCTCTGCTGGGTCTTTGGCTTCCGCTGGTACCCATACATACTCAATTT GCACTAATTTTGTGACACGAGATGAAAAAGCCTTGAGGGGGCGATTAATT CTTGAAGGCAGTTGTGAAGAACATCCGAAGAGGTCAGATCGGTTGAATGT TCGCTTCTTGAGTGGATATATTGAGCCAGATGATGGGCAGGATCTTGACG CATGGTGTGCTGTGATTGGTCAGCGTGATGGAGCATCTAAGAAGAGGCTG AAGGCAAGGGCTGTGGAATTGATGTTGAAGTTGGTGTTTGGATATCGTCC TCCTAGGGGTGGGCTTGGTGAACATGGAGAAATGTCCTACACAATGACTC GTCCCCCCAAAGGGTCTCTTGACATTCTTTATCTGGATGAGGAGCTCCGA GTGACCCGTGGTCAGAGAGGAAGTGTCATAATCGTGGAGCGAGCCTGA
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Coding sequence (CDS) from alignment at C-tenellus_contig8407:2583..3230+

>mRNA_C-tenellus_contig8407.5.1 ID=mRNA_C-tenellus_contig8407.5.1|Name=mRNA_C-tenellus_contig8407.5.1|organism=Choristocarpus tenellus KU2346|type=CDS|length=648bp|location=Sequence derived from alignment at C-tenellus_contig8407:2583..3230+ (Choristocarpus tenellus KU2346)
GGAGAAGATTTTGTTGGCACAAATCGAACGGAAGCAGGGTTGGAATGCAC
ACTAGGTCGGCTTGCTTTCAACATCTTTGAGCCCATTGACCTGCCAATTT
TGATCACTTCCATTGGCAACAAAGTCGGGACAGAGGAAATTCATACTTCT
GCCACAGAATCATCCACTGCCTCTCCTTCTGGGGAAAGGCGTGGAGATGC
GCCCTCTGCTGGGTCTTTGGCTTCCGCTGGTACCCATACATACTCAATTT
GCACTAATTTTGTGACACGAGATGAAAAAGCCTTGAGGGGGCGATTAATT
CTTGAAGGCAGTTGTGAAGAACATCCGAAGAGGTCAGATCGGTTGAATGT
TCGCTTCTTGAGTGGATATATTGAGCCAGATGATGGGCAGGATCTTGACG
CATGGTGTGCTGTGATTGGTCAGCGTGATGGAGCATCTAAGAAGAGGCTG
AAGGCAAGGGCTGTGGAATTGATGTTGAAGTTGGTGTTTGGATATCGTCC
TCCTAGGGGTGGGCTTGGTGAACATGGAGAAATGTCCTACACAATGACTC
GTCCCCCCAAAGGGTCTCTTGACATTCTTTATCTGGATGAGGAGCTCCGA
GTGACCCGTGGTCAGAGAGGAAGTGTCATAATCGTGGAGCGAGCCTGA
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