mRNA_C-tenellus_contig8327.2.1 (mRNA) Choristocarpus tenellus KU2346

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_C-tenellus_contig8327.2.1
Unique NamemRNA_C-tenellus_contig8327.2.1
TypemRNA
OrganismChoristocarpus tenellus KU2346 (Choristocarpus tenellus KU2346)
Homology
BLAST of mRNA_C-tenellus_contig8327.2.1 vs. uniprot
Match: D7FQD4_ECTSI (EF-hand domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FQD4_ECTSI)

HSP 1 Score: 123 bits (309), Expect = 3.860e-31
Identity = 61/146 (41.78%), Postives = 95/146 (65.07%), Query Frame = 3
Query:  159 RLSTRKEREILACFQAFDQGGKGFLDKDDLKCAITALTGSRPTKYEVKRLLDAAIVQEPACPGVTQTFFLESMSVRIAQVDPAEEVREIFRAFDKRGRGFLTKEDLQEVSVSGWRRGIESPQKLSSVGFDACWLV*REFVNGADWL 596
            R++  K +EI  CF  F    K +LDKDDLK A+TAL G RP+K++V+++L  A  ++P CPG+++  F+ESM  R+A VDPAEE+R+IF+AFD+  RGF+T++DL++V    +      P K++ +  +A W    +     DW+
Sbjct:    4 RITESKLQEISDCFNEFSDN-KSYLDKDDLKVAVTALLGFRPSKFDVQQMLALATAEDPQCPGMSRDMFVESMGRRLALVDPAEELRQIFKAFDRGCRGFITRDDLRQVLSEVFSESEMPPVKVAEMFSEADW----DGSGRVDWI 144          
BLAST of mRNA_C-tenellus_contig8327.2.1 vs. uniprot
Match: A0A812C2P9_SEPPH (EF-hand calcium-binding domain-containing protein 11 n=1 Tax=Sepia pharaonis TaxID=158019 RepID=A0A812C2P9_SEPPH)

HSP 1 Score: 77.8 bits (190), Expect = 8.850e-14
Identity = 40/107 (37.38%), Postives = 65/107 (60.75%), Query Frame = 3
Query:  168 TRKEREIL-ACFQAFDQGGKGFLDKDDLKCAITALTGSRPTKYEVKRLLDAAIVQEPACPGVTQTFFLESMSVRIAQVDPAEEVREIFRAFDKRGRGFLTKEDLQEV 485
            ++KE ++L   FQ  D+GGKG+L ++DLK ++  + G +P+KYE  +++      +   PG+    FLE M  ++   D  EE+R+ F AFD +  GFL  EDL++V
Sbjct:   62 SKKETKLLHKVFQDCDEGGKGYLTREDLKVSLVLVFGYKPSKYETDQMMKKYCHGDEINPGLNLEQFLEVMKPKMLVKDEDEEIRQTFMAFDSQCNGFLKVEDLKKV 168          
BLAST of mRNA_C-tenellus_contig8327.2.1 vs. uniprot
Match: A0A401PTM7_SCYTO (Uncharacterized protein n=2 Tax=Scyliorhinus TaxID=7829 RepID=A0A401PTM7_SCYTO)

HSP 1 Score: 75.1 bits (183), Expect = 1.830e-13
Identity = 45/118 (38.14%), Postives = 66/118 (55.93%), Query Frame = 3
Query:  132 CPVKEDRKYRLSTRKEREILACFQAFDQGGKGFLDKDDLKCAITALTGSRPTKYEVKRLLDAAIVQEPACPGVTQTFFLESMSVRIAQVDPAEEVREIFRAFDKRGRGFLTKEDLQEV 485
            C V E+R   ++   +   +  F   D G KG+L ++DLK A+ A+ G +P+K E   ++ AA  QE   PG++   F   MS ++A  D  +E+R IF AFD R RGFLT ED +  
Sbjct:    7 CIVGEERI--VTDSDKSHFVTVFHECDLGQKGYLSREDLKVAVVAIFGYKPSKMETDVVMAAA--QENDLPGLSLEQFTVLMSRKMAAQDRYDEIRHIFSAFDARCRGFLTLEDFKRA 120          
BLAST of mRNA_C-tenellus_contig8327.2.1 vs. uniprot
Match: A0A6G0X8N6_9STRA (Uncharacterized protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0X8N6_9STRA)

HSP 1 Score: 73.6 bits (179), Expect = 1.050e-12
Identity = 38/100 (38.00%), Postives = 60/100 (60.00%), Query Frame = 3
Query:  186 ILACFQAFDQGGKGFLDKDDLKCAITALTGSRPTKYEVKRLLDAAIVQEPACPGVTQTFFLESMSVRIAQVDPAEEVREIFRAFDKRGRGFLTKEDLQEV 485
            +L CF+ +D G KG+L KD++K A+TAL GS PTK  + +L +    Q      + Q  FL+ MS R+  VD  E +R  F+AFD    GF++ ++ +++
Sbjct:    9 VLECFRRYDSGQKGYLIKDEVKWAVTALLGSTPTKLVLVQLFNIPQEQYKDM-RIDQDIFLQVMSRRMENVDVTETIRRWFKAFDTDSSGFISWKNFEKI 107          
BLAST of mRNA_C-tenellus_contig8327.2.1 vs. uniprot
Match: A0A8D2Q7D5_VARKO (EF-hand calcium binding domain 11 n=1 Tax=Varanus komodoensis TaxID=61221 RepID=A0A8D2Q7D5_VARKO)

HSP 1 Score: 73.6 bits (179), Expect = 1.280e-12
Identity = 44/132 (33.33%), Postives = 73/132 (55.30%), Query Frame = 3
Query:  198 FQAFDQGGKGFLDKDDLKCAITALTGSRPTKYEVKRLLDAAIVQEPACPGVTQTFFLESMSVRIAQVDPAEEVREIFRAFDKRGRGFLTKEDLQE------------VSVSGWRRG-IESPQKLSSVGFDAC 554
            F+A D+G KG+L ++D K A+  L G +P+K EV  ++ A+++Q    PG++   FL  MS + A      EVR++F AFD++ +GFL+ ED ++            + +  +RR  I+SP      G + C
Sbjct:   19 FEASDEGNKGYLSREDYKVAVVMLFGYKPSKVEVDSIM-ASVMQSQ--PGLSLEKFLNVMSTKKAARFYHNEVRQLFTAFDRQCKGFLSFEDFKKAFNIVAPKLPERIIIEAFRRSCIKSPASAEKAGKETC 147          
BLAST of mRNA_C-tenellus_contig8327.2.1 vs. uniprot
Match: UPI001CB81A38 (EF-hand calcium-binding domain-containing protein 11 isoform X1 n=1 Tax=Chiloscyllium plagiosum TaxID=36176 RepID=UPI001CB81A38)

HSP 1 Score: 71.6 bits (174), Expect = 7.690e-12
Identity = 43/120 (35.83%), Postives = 66/120 (55.00%), Query Frame = 3
Query:  129 LCPVKEDRKYRLSTRKER-EILACFQAFDQGGKGFLDKDDLKCAITALTGSRPTKYEVKRLLDAAIVQEPACPGVTQTFFLESMSVRIAQVDPAEEVREIFRAFDKRGRGFLTKEDLQEV 485
            LC   E+R   + T  E+   +  F+  D G KG+L ++DLK A+ A  G +P+K E+  ++  A+  E   PG+    F   MS ++A  D  +E+R+IF AFD R  GFLT +D +  
Sbjct:    7 LCTADEER---IVTDLEKCHFVTVFRECDLGQKGYLSREDLKVAVVAAFGYKPSKLEIDMMMAGAV--ENNLPGLPLEQFTALMSRKLAAQDRYDEIRQIFSAFDARCCGFLTLDDFKRA 121          
BLAST of mRNA_C-tenellus_contig8327.2.1 vs. uniprot
Match: A0A388KTV3_CHABU (Uncharacterized protein n=1 Tax=Chara braunii TaxID=69332 RepID=A0A388KTV3_CHABU)

HSP 1 Score: 73.2 bits (178), Expect = 1.100e-11
Identity = 39/101 (38.61%), Postives = 56/101 (55.45%), Query Frame = 3
Query:  183 EILACFQAFDQGGKGFLDKDDLKCAITALTGSRPTKYEVKRLLDAAIVQEPACPGVTQTFFLESMSVRIAQVDPAEEVREIFRAFDKRGRGFLTKEDLQEV 485
            ++   F A+D GGKG L   +LKCA+T LTG +P+K E+     AA +       V Q+ F   +S R+   DP + VR  F AFD RG GF+ ++D +  
Sbjct:  142 DVDGVFSAYDNGGKGSLAPFELKCAMTCLTGIKPSKAEI-----AAFIASAPRGCVDQSTFKRLVSERLQLADPFDSVRHAFHAFDVRGVGFIGRQDFRSA 237          
BLAST of mRNA_C-tenellus_contig8327.2.1 vs. uniprot
Match: UPI001CB7CF47 (EF-hand calcium-binding domain-containing protein 11 isoform X3 n=1 Tax=Chiloscyllium plagiosum TaxID=36176 RepID=UPI001CB7CF47)

HSP 1 Score: 70.9 bits (172), Expect = 1.310e-11
Identity = 39/111 (35.14%), Postives = 61/111 (54.95%), Query Frame = 3
Query:  153 KYRLSTRKEREILACFQAFDQGGKGFLDKDDLKCAITALTGSRPTKYEVKRLLDAAIVQEPACPGVTQTFFLESMSVRIAQVDPAEEVREIFRAFDKRGRGFLTKEDLQEV 485
            K + S  +   +   F+  D G KG+L ++DLK A+ A  G +P+K E+  ++  A+  E   PG+    F   MS ++A  D  +E+R+IF AFD R  GFLT +D +  
Sbjct:   18 KLQQSVHRLHPVAFVFRECDLGQKGYLSREDLKVAVVAAFGYKPSKLEIDMMMAGAV--ENNLPGLPLEQFTALMSRKLAAQDRYDEIRQIFSAFDARCCGFLTLDDFKRA 126          
BLAST of mRNA_C-tenellus_contig8327.2.1 vs. uniprot
Match: UPI001CB853C2 (EF-hand calcium-binding domain-containing protein 11 isoform X2 n=1 Tax=Chiloscyllium plagiosum TaxID=36176 RepID=UPI001CB853C2)

HSP 1 Score: 70.9 bits (172), Expect = 1.370e-11
Identity = 39/111 (35.14%), Postives = 61/111 (54.95%), Query Frame = 3
Query:  153 KYRLSTRKEREILACFQAFDQGGKGFLDKDDLKCAITALTGSRPTKYEVKRLLDAAIVQEPACPGVTQTFFLESMSVRIAQVDPAEEVREIFRAFDKRGRGFLTKEDLQEV 485
            K + S  +   +   F+  D G KG+L ++DLK A+ A  G +P+K E+  ++  A+  E   PG+    F   MS ++A  D  +E+R+IF AFD R  GFLT +D +  
Sbjct:   18 KLQQSVHRLHPVAFVFRECDLGQKGYLSREDLKVAVVAAFGYKPSKLEIDMMMAGAV--ENNLPGLPLEQFTALMSRKLAAQDRYDEIRQIFSAFDARCCGFLTLDDFKRA 126          
BLAST of mRNA_C-tenellus_contig8327.2.1 vs. uniprot
Match: A0A7S3V2M3_9STRA (Hypothetical protein n=1 Tax=Aplanochytrium stocchinoi TaxID=215587 RepID=A0A7S3V2M3_9STRA)

HSP 1 Score: 70.5 bits (171), Expect = 1.750e-11
Identity = 43/120 (35.83%), Postives = 65/120 (54.17%), Query Frame = 3
Query:  186 ILACFQAFDQGGKGFLDKDDLKCAITALTGSRPTKYEVKRLL----------------------DAAIVQEPACPG--VTQTFFLESMSVRIAQVDPAEEVREIFRAFDKRGRGFLTKED 473
            + ACF+ FD+G KGFL+K DLK A+ AL G RPTK+EV+++L                      D + +++    G  + +  F + M  ++  VD  + +R++F AFD   RGFLT ED
Sbjct:    7 VRACFRDFDKGNKGFLNKHDLKFAMAALFGIRPTKFEVEQILSIAKDYVIRLEQDRQKNSETVHDNSGLEDTEIEGARIYEPIFAQIMMSKLELVDEDDILRQVFCAFDVGCRGFLTFED 126          
The following BLAST results are available for this feature:
BLAST of mRNA_C-tenellus_contig8327.2.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FQD4_ECTSI3.860e-3141.78EF-hand domain-containing protein n=1 Tax=Ectocarp... [more]
A0A812C2P9_SEPPH8.850e-1437.38EF-hand calcium-binding domain-containing protein ... [more]
A0A401PTM7_SCYTO1.830e-1338.14Uncharacterized protein n=2 Tax=Scyliorhinus TaxID... [more]
A0A6G0X8N6_9STRA1.050e-1238.00Uncharacterized protein n=1 Tax=Aphanomyces euteic... [more]
A0A8D2Q7D5_VARKO1.280e-1233.33EF-hand calcium binding domain 11 n=1 Tax=Varanus ... [more]
UPI001CB81A387.690e-1235.83EF-hand calcium-binding domain-containing protein ... [more]
A0A388KTV3_CHABU1.100e-1138.61Uncharacterized protein n=1 Tax=Chara braunii TaxI... [more]
UPI001CB7CF471.310e-1135.14EF-hand calcium-binding domain-containing protein ... [more]
UPI001CB853C21.370e-1135.14EF-hand calcium-binding domain-containing protein ... [more]
A0A7S3V2M3_9STRA1.750e-1135.83Hypothetical protein n=1 Tax=Aplanochytrium stocch... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
C-tenellus_contig8327contigC-tenellus_contig8327:562..2517 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Choristocarpus tenellus KU2346 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog2880.D7FQD4
Preferred nameEFCAB11
PFAMsEF-hand_1,EF-hand_7,EF-hand_8
Model size731
Max annot lvl2759|Eukaryota
KEGG koko:K02183,ko:K16465
KEGG Pathwayko04014,ko04015,ko04016,ko04020,ko04022,ko04024,ko04070,ko04114,ko04218,ko04261,ko04270,ko04371,ko04626,ko04713,ko04720,ko04722,ko04728,ko04740,ko04744,ko04745,ko04750,ko04910,ko04912,ko04915,ko04916,ko04921,ko04922,ko04924,ko04925,ko04970,ko04971,ko05010,ko05031,ko05034,ko05133,ko05152,ko05167,ko05200,ko05214,ko05418,map04014,map04015,map04016,map04020,map04022,map04024,map04070,map04114,map04218,map04261,map04270,map04371,map04626,map04713,map04720,map04722,map04728,map04740,map04744,map04745,map04750,map04910,map04912,map04915,map04916,map04921,map04922,map04924,map04925,map04970,map04971,map05010,map05031,map05034,map05133,map05152,map05167,map05200,map05214,map05418
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0005488,GO:0005509,GO:0043167,GO:0043169,GO:0046872
Exons4
Evalue8.63e-33
EggNOG OGsCOG5126@1|root,KOG0027@2759|Eukaryota
DescriptionCalcium-binding protein
Cds size537
COG categoryDTZ
BRITEko00000,ko00001,ko01009,ko03036,ko04131,ko04147
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_C-tenellus_contig8327.2.1prot_C-tenellus_contig8327.2.1Choristocarpus tenellus KU2346polypeptideC-tenellus_contig8327 727..2488 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680790098.2757113-UTR-C-tenellus_contig8327:561..7261680790098.2757113-UTR-C-tenellus_contig8327:561..726Choristocarpus tenellus KU2346UTRC-tenellus_contig8327 562..726 -
1680790098.3473582-UTR-C-tenellus_contig8327:2488..25171680790098.3473582-UTR-C-tenellus_contig8327:2488..2517Choristocarpus tenellus KU2346UTRC-tenellus_contig8327 2489..2517 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680790098.2869275-CDS-C-tenellus_contig8327:726..9471680790098.2869275-CDS-C-tenellus_contig8327:726..947Choristocarpus tenellus KU2346CDSC-tenellus_contig8327 727..947 -
1680790098.3029609-CDS-C-tenellus_contig8327:1692..17471680790098.3029609-CDS-C-tenellus_contig8327:1692..1747Choristocarpus tenellus KU2346CDSC-tenellus_contig8327 1693..1747 -
1680790098.3174884-CDS-C-tenellus_contig8327:2031..20961680790098.3174884-CDS-C-tenellus_contig8327:2031..2096Choristocarpus tenellus KU2346CDSC-tenellus_contig8327 2032..2096 -
1680790098.3333077-CDS-C-tenellus_contig8327:2292..24881680790098.3333077-CDS-C-tenellus_contig8327:2292..2488Choristocarpus tenellus KU2346CDSC-tenellus_contig8327 2293..2488 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_C-tenellus_contig8327.2.1

>prot_C-tenellus_contig8327.2.1 ID=prot_C-tenellus_contig8327.2.1|Name=mRNA_C-tenellus_contig8327.2.1|organism=Choristocarpus tenellus KU2346|type=polypeptide|length=179bp
MQGSGCANEGVSPLLPGTGRRGVFEASTSRLHTLCPVKEDRKYRLSTRKE
REILACFQAFDQGGKGFLDKDDLKCAITALTGSRPTKYEVKRLLDAAIVQ
EPACPGVTQTFFLESMSVRIAQVDPAEEVREIFRAFDKRGRGFLTKEDLQ
EVSVSGWRRGIESPQKLSSVGFDACWLV*
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mRNA from alignment at C-tenellus_contig8327:562..2517-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_C-tenellus_contig8327.2.1 ID=mRNA_C-tenellus_contig8327.2.1|Name=mRNA_C-tenellus_contig8327.2.1|organism=Choristocarpus tenellus KU2346|type=mRNA|length=1956bp|location=Sequence derived from alignment at C-tenellus_contig8327:562..2517- (Choristocarpus tenellus KU2346)
TTTCAAAGTTGACTGGTTCAGTAGTGTGAATGCAGGGGAGTGGTTGTGCC AATGAAGGTGTCTCACCGCTACTTCCTGGTACTGGAAGGAGAGGGGTATT TGAGGCTTCAACGTCGCGATTACATACGCTGTGTCCTGTAAAGGAGGATC GAAAATATCGGCTCAGTACCAGGAAGGAGAGGGAAATATTGGCTTGCTTC CAGGCATTCGACCAGGGTGGGAAAGGTGCTGAAGTGACGAATCAAGAGAA ATGGGTGTTTCAGTGTCCCTTTTTGAAATCCTAACAAATGTATTTTCCAG AGAAGGGATGATGGTGAGGTCACGGTAAAGGACGATGAGCATGGAGTTCA GGCTGTGTTGTCGCTACCCCGCCTAGTGATCCTATATGTGCGTGCTAATG AACTTCGCTCGTGGTATTCAGGTTTTTTGGACAAGGATGATCTCAAGTGC GCAATTACAGCGCTTACGGGATCCCGTCCCACGAAGGTGACTTGGTTGAC GGTTTGCAGTGTTTGATGTGGTGTGGTGATCGTATCTATATTTTCTTGTC CAAATCCTTGTGCAAAGAAGATAGAAGTTAGAAACGATAAATTCAGCGGT AACGATCAACAATGCAAGGGGGGTTGTCTTTCCTCGCAACCGTATTTGCA GCACGAGAGGAGCGTGTTTCTGCAGGAGGCCCCTAATCAAGTTGGCTGTA CACGCAAGCACTCACGTCATCTAACCCACACTTGTCACCTAATTGCCCAA CCCACTTATACACGATGCAGTACGAGGTGAAGCGACTGCTCGACGCAGCG ATCGTACAGGAACCAGCATGTCCAGGTTGGTGAAAGAGGTGGTGTTGCCA CGTGAATTTTAAACATGGAAAATTCTAGCCATGTTTCTATCATTCTCCTT CATGTTTTTCGTATGATCTTGAGTGCTCACTTAACCCTGATACACTGTTT CCTTCATCTTTTGTTAAGTCAAAGATCCATGATCATCCAATGCATTGCCG AGCACTTGTTTGGAGTGCCCCTTCAGTGAATCTTACTTGCCAAAAGTGAT GGGGCCTTCACACTCTTTGGCAAGTCCCTACTATCTTCAGTGCTCCTTTT ACCTTCCTCAATACTTTCAAAATGTTTAATCATGTTGTAAGACAAGCTTT TAAACTGTGTTTTGTTGTTTGATGCCAAAATCTTTACAAGTAGCCGCAGT AGTGCAGCAGCAGGAAGGCCACCAGTTGTTTTGTCAAGGGCAAACACCAC AGAACAGAATAAGCTACCTGCCTAGTGCAATTTTTGTGATTGTTGGAGTG CTTGCAAAGCATTTCAAGTTTTTGAGACAATATACGTCTTCAATCCCGGC TGCTACAAAATTAACATCAGATGCAATATTATGTTGTACTTTTGTTGGGT GGAGTCCTCCTTGGGCGTGATGTTAATGTTGTTGCCTTGAGTAGAGGAAG AGTGGAAGTCAGGCCTGCTCTGGCTGCCAACACGTTGCCTTTTACCTCAA GACCTCATAAAGTATATTTTGGCCCTATCACCACTGTGATGATTGCAATG ACTACCGTTATCACCTTCAGGTGTCACTCAGACCTTCTTCTTGGAGTCTA TGTCGGTTCGCATTGCCCAGGTGGACCCAGCAGAGGAAGTTCGAGAAATA TTTAGGGCATTCGACAAGAGGGGCCGTGGATTCCTCACAAAAGAGGACCT GCAGGAGGTGAGTGTCTCAGGATGGAGGAGGGGAATAGAATCACCCCAAA AATTGTCTTCGGTGGGTTTTGATGCATGCTGGTTGGTTTGAAGAGAATTT GTGAATGGTGCTGATTGGTTGATTATCGTTGTCAATTGTGTGTGGTTTGC GTGCACCACGAATTCCTGCCTTTTCATTGGTCCACAATGTTGTCGTCCCT GGTAGTATTTGGATGTTCATTAGTGGACAGTGGTCCTGTCTTATTCCGGG AGTTAC
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Coding sequence (CDS) from alignment at C-tenellus_contig8327:562..2517-

>mRNA_C-tenellus_contig8327.2.1 ID=mRNA_C-tenellus_contig8327.2.1|Name=mRNA_C-tenellus_contig8327.2.1|organism=Choristocarpus tenellus KU2346|type=CDS|length=537bp|location=Sequence derived from alignment at C-tenellus_contig8327:562..2517- (Choristocarpus tenellus KU2346)
ATGCAGGGGAGTGGTTGTGCCAATGAAGGTGTCTCACCGCTACTTCCTGG
TACTGGAAGGAGAGGGGTATTTGAGGCTTCAACGTCGCGATTACATACGC
TGTGTCCTGTAAAGGAGGATCGAAAATATCGGCTCAGTACCAGGAAGGAG
AGGGAAATATTGGCTTGCTTCCAGGCATTCGACCAGGGTGGGAAAGGTTT
TTTGGACAAGGATGATCTCAAGTGCGCAATTACAGCGCTTACGGGATCCC
GTCCCACGAAGTACGAGGTGAAGCGACTGCTCGACGCAGCGATCGTACAG
GAACCAGCATGTCCAGGTGTCACTCAGACCTTCTTCTTGGAGTCTATGTC
GGTTCGCATTGCCCAGGTGGACCCAGCAGAGGAAGTTCGAGAAATATTTA
GGGCATTCGACAAGAGGGGCCGTGGATTCCTCACAAAAGAGGACCTGCAG
GAGGTGAGTGTCTCAGGATGGAGGAGGGGAATAGAATCACCCCAAAAATT
GTCTTCGGTGGGTTTTGATGCATGCTGGTTGGTTTGA
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