mRNA_C-tenellus_contig7721.1.1 (mRNA) Choristocarpus tenellus KU2346

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_C-tenellus_contig7721.1.1
Unique NamemRNA_C-tenellus_contig7721.1.1
TypemRNA
OrganismChoristocarpus tenellus KU2346 (Choristocarpus tenellus KU2346)
Homology
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BLAST of mRNA_C-tenellus_contig7721.1.1 vs. uniprot
Match: D7FL06_ECTSI (TLDc domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FL06_ECTSI)

HSP 1 Score: 292 bits (748), Expect = 3.630e-91
Identity = 142/218 (65.14%), Postives = 172/218 (78.90%), Query Frame = 1 
Query:    1 GTDVSESLYEWAMHYRGVALLGGLALLLSTGKFISYYWDRIPLLRRTVIPPAAIAGLLGCAIYRVFSGLLEASGVKSLVDEGLSEVVVNLINFTFVAFMLGFASMGTYVPRTRVVLHTIWHEAMPMLIYSQVLIWGQSCMALGVVGLFRLGNPEVSPYLGALVTLGLETGRDVTAFSSARIVGEWAHEAVQLADSIGLLLSILAGVVLVTLHKQGQWG 654
            G D  E+L  WA+ YRGVA  G LA+LL TGKF+ ++W R+  LRR V+PPA I+GLLGC +YR+F G++   GVKS +DE LSEVVV +INFTF A  LGFAS GTYVPRTR VL +IWHEA+PML+YSQVLIWGQSC+AL  VGL RLGNP+VS +LGALVT+GLETGRDV +FS+  I GEWA E V+LADS+GLLLS+  G+ L+T+H  G WG
Sbjct:    5 GADDDETLGAWALRYRGVATFGALAVLLGTGKFVGFHWHRLAFLRRFVVPPAVISGLLGCVVYRLFKGVMSP-GVKSSLDESLSEVVVLMINFTFSALTLGFASAGTYVPRTRAVLASIWHEALPMLVYSQVLIWGQSCVALLTVGLIRLGNPDVSVFLGALVTMGLETGRDVMSFSTREIAGEWADEVVRLADSLGLLLSVFGGICLMTMH-MGSWG 220
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BLAST of mRNA_C-tenellus_contig7721.1.1 vs. uniprot
Match: A0A835ZFP8_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZFP8_9STRA)

HSP 1 Score: 179 bits (454), Expect = 2.050e-51
Identity = 95/205 (46.34%), Postives = 131/205 (63.90%), Query Frame = 1 
Query:   28 EWAMHYRGVALLGGLALLLSTGKFISYYWDRIPLLRRTVIPPAAIAGLLGCAIYRVFSGLLEASGVKSLVDEGLSEVVVNLINFTFVAFMLGFASMGTYVPRTRVVLHTIWHEAMPMLIYSQVLIWGQSCMALGVVGLFRLGNPEVSPYLGALVTLGLETGRDVTAFSSARIVGEWAHEAVQLADSIGLLLSILAGVVLVTLHKQ 642
            +WA  Y GVALLG L++LL+ GK I+++   +PLLRR  +PPA +AGLLGC ++    G    + +++ V+E + +VVVNLIN  F    LGF +  TYVP TR +L  IWHEA+PML+YSQVL+WGQSC+AL V  LF   NP V+PYLGAL   G   G               AH+ V+ AD++GL++S+ A V ++T +  
Sbjct:    2 QWAGRYSGVALLGVLSVLLAAGKGINHHMGHVPLLRRLTVPPAVVAGLLGCFLFHTI-GKQFPTELENSVEERMGDVVVNLINLCFAGLALGFGAAKTYVPHTRAILMGIWHEALPMLLYSQVLLWGQSCVALMVGALFSAHNPAVTPYLGALRKGGGAGG------------AADAHDIVRQADAVGLMVSLAASVAVMTWNSM 193
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BLAST of mRNA_C-tenellus_contig7721.1.1 vs. uniprot
Match: A0A4D9DA04_9STRA (TLDc domain-containing protein n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9DA04_9STRA)

HSP 1 Score: 131 bits (330), Expect = 4.580e-32
Identity = 86/206 (41.75%), Postives = 119/206 (57.77%), Query Frame = 1 
Query:   28 EWAMHYRGVALLGGLALLLSTGKFISYYWDRIPLLRRTVIPPAAIAGLLGCAIYRVFSGLLEASGVKSLVDEGLSEVVVNLINFTFVAFMLGFASMGTYVPRT--RVVLHTIWHEAMPMLIYSQVLIWGQSCMALGVVGLFRLGNPEVSPYLGALVTLGLETGRDVTAFSSARIVGEWAHEAVQLADSIGLLLSILAGVVLVTLHK 639
            EWA  Y  +A +G L+ LL  GK + +   ++   R T  P A I+G LG A+Y +     +   V+  ++  L  + VN++NF F A +LGF S GT   +   + +   I+HE MPML+Y+Q LIWG S +AL V G++RL N E+S + G LVTLGLE GRD+    S     E A   V LADS GL +SI+ GVVLV   +
Sbjct:   29 EWAARYEMIAAMGYLSALLMVGKAL-HNLGKLAKFRFTP-PSAVISGFLGLALYDMMGAGSKLLQVE--LESTLRSIEVNIVNFVFTALVLGFTS-GTKKNKMTYKRIFGLIFHEGMPMLLYAQALIWGNSVVALLVTGIYRLWNTEISAFEGGLVTLGLEAGRDL--LPSRVKFKEEALAVVHLADSAGLTISIIVGVVLVACRR 227
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BLAST of mRNA_C-tenellus_contig7721.1.1 vs. uniprot
Match: W7TMX7_9STRA (Uncharacterized protein (Fragment) n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TMX7_9STRA)

HSP 1 Score: 113 bits (283), Expect = 8.400e-28
Identity = 69/166 (41.57%), Postives = 99/166 (59.64%), Query Frame = 1 
Query:   28 EWAMHYRGVALLGGLALLLSTGKFISYYWDRIPLLRRTVIPPAAIAGLLGCAIYRVFSGLLEASGVKSLVDEGLSEVVVNLINFTFVAFMLGFASMGTYVPRT--RVVLHTIWHEAMPMLIYSQVLIWGQSCMALGVVGLFRLGNPEVSPYLGALVTLGLETGRDV 519
            EWA  Y  +A +G L+ LL  GK + +   ++   R T  P A I+G LG A+Y +     +   V+  ++  L  + VN++NF F A +LGF S GT   +   + +   I+HE MPML+Y+Q LIWG S +AL V G++RL N E+S + G LVTLGLE GRD+
Sbjct:   29 EWAARYEMIAAMGYLSALLMVGKAL-HNLGKLAKFRFTP-PSAVISGFLGLALYDMMGAGSKLLQVE--LESTLRSIEVNIVNFVFTALVLGFTS-GTKKNKMTYKRIFGLIFHEGMPMLLYAQALIWGNSVVALLVTGIYRLWNKEISAFEGGLVTLGLEAGRDL 189
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BLAST of mRNA_C-tenellus_contig7721.1.1 vs. uniprot
Match: A0A482RZP7_9ARCH (Uncharacterized protein n=1 Tax=archaeon TaxID=1906665 RepID=A0A482RZP7_9ARCH)

HSP 1 Score: 82.0 bits (201), Expect = 7.040e-16
Identity = 58/166 (34.94%), Postives = 87/166 (52.41%), Query Frame = 1 
Query:   16 ESLYEWAMHYRGVALLGGLALLLSTGKFISYYWDRIPLL-RRTVIPPAAIAGLLGCAIYRVFSGLLEASGVKSLVDE----GLSEVVVNLINFTFVAFMLGFASMGTYVPRT---RVVLHTIWHEAMPMLIYSQVLIWGQSCMALGVVGLFRLGNPEVSPYLGALV 489
            E +  WA  + G ALLG ++ LL  GK   Y   R  LL    V+PP  I+G+LG  +      L+    V  ++ E    GL  +  NL++F F AF LG  S  ++   T   R ++ +I HE MPM+IYSQVL+WGQ+   + V  + R+   +V   L +++
Sbjct:    4 EDVEAWARSFGGTALLGLISALLLVGKLTHYVCQRRGLLCGLLVVPPVLISGVLGLLL------LVTLDHVDPIMAEDVRVGLESLRRNLVSFVFAAFALGLMSTKSHSLHTSSLRGLVVSILHEGMPMMIYSQVLLWGQTVCCMLVYMVTRIVTGQVGVVLLSII 163
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BLAST of mRNA_C-tenellus_contig7721.1.1 vs. uniprot
Match: A0A7S3GR50_9STRA (Hypothetical protein (Fragment) n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3GR50_9STRA)

HSP 1 Score: 61.2 bits (147), Expect = 1.920e-7
Identity = 36/98 (36.73%), Postives = 58/98 (59.18%), Query Frame = 1 
Query:  337 RVVLHTIWHEAMPMLIYSQVLIWGQS--CMALGVVGLFRLGNPEVSPYLGALVTLGLETGRDVTAFSSARIVGEWAHEAVQLADSIGLLLSILAGVVL 624
            R ++ +++HE MPM+IYSQ+L WGQ+  C+    +  F  G    S +  A+V LG+E G D+   S+     +W    V+ A+S+GLL   ++GV+L
Sbjct:   20 RGIVTSLFHEGMPMVIYSQLLNWGQTTCCLLFVCISNFIFGMGIPSAF-AAMVPLGIEAGADIVLSSNYNT--DWTSSIVEEAESLGLLAITVSGVLL 114
The following BLAST results are available for this feature:
BLAST of mRNA_C-tenellus_contig7721.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 6
Match NameE-valueIdentityDescription
D7FL06_ECTSI3.630e-9165.14TLDc domain-containing protein n=2 Tax=Ectocarpus ... [more]
A0A835ZFP8_9STRA2.050e-5146.34Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A4D9DA04_9STRA4.580e-3241.75TLDc domain-containing protein n=1 Tax=Nannochloro... [more]
W7TMX7_9STRA8.400e-2841.57Uncharacterized protein (Fragment) n=1 Tax=Nannoch... [more]
A0A482RZP7_9ARCH7.040e-1634.94Uncharacterized protein n=1 Tax=archaeon TaxID=190... [more]
A0A7S3GR50_9STRA1.920e-736.73Hypothetical protein (Fragment) n=1 Tax=Spumella e... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
C-tenellus_contig7721contigC-tenellus_contig7721:67..3879 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Choristocarpus tenellus KU2346 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog2880.D7FL06
PFAMsELMO_CED12,RUN,RhoGAP,TLD
Model size654
Max annot lvl2759|Eukaryota
Hectar predicted targeting categoryother localisation
Exons4
Evalue5.96e-92
EggNOG OGsCOG5142@1|root,KOG2372@2759|Eukaryota
Ec32 ortholog descriptionTLDc domain
Ec32 orthologEc-21_002090.1
Descriptionnegative regulation of peptidyl-cysteine S-nitrosylation
Cds size654
COG categoryL
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680790054.2887378-CDS-C-tenellus_contig7721:66..1561680790054.2887378-CDS-C-tenellus_contig7721:66..156Choristocarpus tenellus KU2346CDSC-tenellus_contig7721 67..156 -
1680790054.3030539-CDS-C-tenellus_contig7721:669..8401680790054.3030539-CDS-C-tenellus_contig7721:669..840Choristocarpus tenellus KU2346CDSC-tenellus_contig7721 670..840 -
1680790054.313475-CDS-C-tenellus_contig7721:1510..16481680790054.313475-CDS-C-tenellus_contig7721:1510..1648Choristocarpus tenellus KU2346CDSC-tenellus_contig7721 1511..1648 -
1680790054.3261712-CDS-C-tenellus_contig7721:3624..38791680790054.3261712-CDS-C-tenellus_contig7721:3624..3879Choristocarpus tenellus KU2346CDSC-tenellus_contig7721 3625..3879 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_C-tenellus_contig7721.1.1prot_C-tenellus_contig7721.1.1Choristocarpus tenellus KU2346polypeptideC-tenellus_contig7721 67..3879 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_C-tenellus_contig7721.1.1

>prot_C-tenellus_contig7721.1.1 ID=prot_C-tenellus_contig7721.1.1|Name=mRNA_C-tenellus_contig7721.1.1|organism=Choristocarpus tenellus KU2346|type=polypeptide|length=218bp
GTDVSESLYEWAMHYRGVALLGGLALLLSTGKFISYYWDRIPLLRRTVIP
PAAIAGLLGCAIYRVFSGLLEASGVKSLVDEGLSEVVVNLINFTFVAFML
GFASMGTYVPRTRVVLHTIWHEAMPMLIYSQVLIWGQSCMALGVVGLFRL
GNPEVSPYLGALVTLGLETGRDVTAFSSARIVGEWAHEAVQLADSIGLLL
SILAGVVLVTLHKQGQWG
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mRNA from alignment at C-tenellus_contig7721:67..3879-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_C-tenellus_contig7721.1.1 ID=mRNA_C-tenellus_contig7721.1.1|Name=mRNA_C-tenellus_contig7721.1.1|organism=Choristocarpus tenellus KU2346|type=mRNA|length=3813bp|location=Sequence derived from alignment at C-tenellus_contig7721:67..3879- (Choristocarpus tenellus KU2346)
GGCACAGATGTTAGCGAGAGTCTGTATGAGTGGGCCATGCACTACCGAGG
GGTGGCCCTCCTTGGTGGCTTGGCCCTTTTACTGTCAACTGGAAAGTTTA
TCAGCTATTACTGGGATCGAATCCCTTTGTTGCGCAGGACGGTGATTCCA
CCGGCAGCCATCGCTGGTCTGCTGGGGTGCGCTATCTACCGCGTTTTCAG
CGGACTGCTTGAAGCTTCTGGAGTGAAGAGTCTGGTGGATGAGGGCCTCT
CCGAGGTGATTACTCCAGAGTGGGCTGAGGCTTTTATGTGTACTCATGCT
CGCATTCTTTTTGTTTGGATTACGAGAGAGACGTTTGTATCTGCAGTCGC
TTGCATCTGTCCTGGGAGTGAAAGAGGGATTGGTCTTGATCGTGCATTAC
TTCAGTTTGCTTACCTGCTGAAGTTAGGGGTTTGCCTGTTGACAGCAAGC
TACATAGGATAAATCATGAGTGTGAGTTTTTCATAGTGACGGTGAGATTA
CTTTCGGTTTGACAGTCTGGATTGTGCCATGTCTTGAATCCAATCGAGAT
AGAGGAGGAATCTTCTGTTTTAATCTTTTTTAACAATCACAATCATGGCA
AGGTAGCACACAAGGTCGTACTAACTTTGCAGTGCTTGCAATCTCTAAGA
TTTTGTCCCCAGCTGAGATCCGAATTGCAATATGTCCAATGCTGCAAGTA
CACTGGATTAGCCCCTTCCTCATCCTGCATCAACCCGCGCGTGTACGTGT
ACTTACAAGGAAAACAACGTGTCTCTCGTTCACATATTAAACTATGCTCT
ATACTTGTAATATCTGATGGAAAGGGTGAGATCCCAATCAGAGCTGACGT
ACTTTGTGATCCAAAACAATCACTATTTTTTCTCTCTTGGTTCTAAAGAG
CACCTTTTCTGTGATTTTGATAGATTTATCCTGTTGACCAGAGGTTAGAG
TTTCAGAGCACAGGATAGGGGAAGATCTAATTATGCGTCGTATGTACCCG
ACTGTGTTTGATTGTAGGGCCATTTACTGTTTCGCATCGAATAAACTACT
CGGGACTCGGCACTGTTCAACATTCCCGTCATCCACCCTGTCGGCGAAGT
CCATCTGGGCACTCATATGCAAAAGCAGTTCCCAATAAAGCACCACCCTT
TGCAATTTCTCCCCTTGTTTTCAACACTTTGATGAAGTTGTGAGGTCTAT
CAGCAGACTGATCGCGTAGATTATTTCTTGCAGATACAGACAGATTCTGC
CCAACCACTGTAGGACTATGTATTAGCAGTAGAGTAGTAGAAGCAGCAGC
AGTGGTAACAATATTTGCAGTTTTTAAAAGCAAGCATTTTAAAAGAGTCA
GTACCCGTGAAGAGTTCGACACGGCTTGTGGTCTTGTGCTTCTGGATGAC
CTTGGGCCACCCCAGACACACCACAAGACCTGCATGTCCTCTTTCTTGTA
GTCTTTTGCACCTGGATAGCCAATACATTCACTCCAATATGCCACACACG
CCTCCACGATCTATCCTGTTTCTCCCTCAGTTTGCATCCCAACCAGTGAA
AACGCAATGGAGGACTCTTGTTCAAGTTCATTAGGCAACACTTGTTCTCA
TTTTGTTGTGTGTGTGTGTGTCTGTGTTGTGTCCTGGCTGGTGTATGCCC
CATCGAGTTAGTCGTTGCTTTTTAGGATCAAGAGGGGTTTTTTCTTACCC
TATCAACAGTGCTGATGCGTATTGCAGGTGTACCTTTTTTTGTCTCGCAA
CCTTGTCTATTCGTTCTATCCCCCGGTATTTTGTAGCGTGATTATTTTCA
ACACATTTGCAACTATGAGTTAATAACTCTCTACAACGCCTGACCCTTTC
GTCGAACTCTTATGTTTGGTTGCACACCCACCACCTGACGAGGAGAGTAT
TTTTTTTTATCCTTGGGGTGGGTGCATAGTAGTAAAGTGCACTGGAGTAC
GGCATCGCACAGTAATTTTTTTCTTTATCTGATGTGCACTGTCTTTATAA
CATTCATTGCCTTACGAAAATATAGTTGAGGTGCTGCTGCTGTCGTGCAG
CTGAAATACGTAATGTTTAAATAACACGCATGCTTTTTTTTTTACACTCT
TTTTGCAGTACGGTTTGTAGTCACTATCAATGTTTAAAAATATATATTAT
TTTTTTATGTGTGTGCGTAAGTGTGTGTCCCCTTGCCCCCCCACACCCTG
CTCTGACGTTCGCTGTTGGACACGCAAAAAGGTGGTGGTAAACCTGATCA
ATTTCACGTTTGTGGCTTTTATGCTCGGGTTTGCAAGCATGGGGACCTAC
GTACCCCGAACCCGGGTTGTCCTTCACACCATTTGGCACGAGGCCATGCC
CATGCTCATCTATAGTCAGGTGCGGCATGTCGGTTGGGATCGCGAGCACG
TTCACCCTTTTCTTTGTATCCATGGTTGAGAGAGTTTTATTGTTCGTGAG
TTAGGGAGGGAGGCTTTGTTGCACAGTGGGGCTTGGCGTCGTCTCCTGTC
AGTTTTTGGTTAGCTCGTGTGCTTGATTTTTTCAGGTGGGGGCAGGGGGG
GCGGGGGTTAATTCTAGGACACTAAAGTTGGTGTTCTATGCATCATGTAT
TTGAGGCACGAGAAGGAGACTCGGGAGAAGAGGGTCTGGGATGGGAGCAG
AGGAGGGTGGTTTTGGTGCCTTGAGCCTTTGTAATGATGGTTGAGTGCAT
TACTGTTTGTTTGCTACTCACAGCGGTGCCGTCGTTTATTTGTTGTAATA
TTTAAAGGAGTAGGAAATGTTGCAGGGTGAATGAAGGCATCTTATTGGTG
CCATGTGCGGTACGGTCCGTCCCATTGGATGAGATGTTGCCTTTCGTGCG
TGCACAGTACCCCCATGAAAATCCAGACTCCCACCCCCATAGCAACTGTT
CTCTTTGCTCGTGTCATCAAGAACAAATGTACTCTATGAAAGCCACATGC
TTAGTTAGTGCCTTAAGATTCACATTTATTACCACTCTTCCCCCCCACCT
ATTCCTCTCTGCTCTTTCCAACACTACCTACCTTGATAGGTACTCATTTG
GGGGCAGTCTTGTATGGCACTGGGAGTGGTTGGCCTCTTCCGACTCGGCA
ACCCTGAGGTTTCCCCATACTTGGGAGCGTTGGTCACACTGGGATTGGAG
ACTGGACGTGATGTAACTGCTTTTTCTTCGGCTCGCATTGTGGGGGAGTG
GGCACATGAGGTGATCAGTGACAGTGATACGGTGAACAGAGAGAATATGT
GGTTATACCTGGTAAATTTCCATCTGTTGGCTTGGTCCTCAGGTTGGTGA
GAGACAGTCTGGTCCTGGTTCAAGGAATACTATGAAGGGTAATAGTTGAA
GGGAGTAAGTACCCCCACAGTGCTGTTGGTGGCATCCAGTTCAAAATATG
TTGGAGGTTTAATGAAAGTACATAGTGAGTGTACGTGTGAAGGTCATGAC
CCTCTTTTTTTTGCTTTTTTTTATGAGTGTGTATCAGTGCTGAAGTGGGC
GTGATCGTGAAATGATACGTGTGTCTCCATTTCTTAAGCGGCTCTTTAGA
TGAGGCTTTGGTGTACCTTTTTTGTGGTGTGACAGGAGTTCCTTTTAAGG
GTATAGTGTACACTAATGTACTGTGTAGAACAGATAATACGGGATGGGGA
GTTGTGAGGAACCTTGGTCACCATGGCGTGTGGCGTGTTTGCCAATGCGT
ACCCTCTCTACATCATTGATCAGGCGGTGCAACTAGCGGATTCCATTGGG
CTTCTCCTGTCGATCCTTGCGGGTGTTGTCCTTGTAACACTCCACAAACA
GGGGCAATGGGGA
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Coding sequence (CDS) from alignment at C-tenellus_contig7721:67..3879-

>mRNA_C-tenellus_contig7721.1.1 ID=mRNA_C-tenellus_contig7721.1.1|Name=mRNA_C-tenellus_contig7721.1.1|organism=Choristocarpus tenellus KU2346|type=CDS|length=654bp|location=Sequence derived from alignment at C-tenellus_contig7721:67..3879- (Choristocarpus tenellus KU2346)
GGCACAGATGTTAGCGAGAGTCTGTATGAGTGGGCCATGCACTACCGAGG
GGTGGCCCTCCTTGGTGGCTTGGCCCTTTTACTGTCAACTGGAAAGTTTA
TCAGCTATTACTGGGATCGAATCCCTTTGTTGCGCAGGACGGTGATTCCA
CCGGCAGCCATCGCTGGTCTGCTGGGGTGCGCTATCTACCGCGTTTTCAG
CGGACTGCTTGAAGCTTCTGGAGTGAAGAGTCTGGTGGATGAGGGCCTCT
CCGAGGTGGTGGTAAACCTGATCAATTTCACGTTTGTGGCTTTTATGCTC
GGGTTTGCAAGCATGGGGACCTACGTACCCCGAACCCGGGTTGTCCTTCA
CACCATTTGGCACGAGGCCATGCCCATGCTCATCTATAGTCAGGTACTCA
TTTGGGGGCAGTCTTGTATGGCACTGGGAGTGGTTGGCCTCTTCCGACTC
GGCAACCCTGAGGTTTCCCCATACTTGGGAGCGTTGGTCACACTGGGATT
GGAGACTGGACGTGATGTAACTGCTTTTTCTTCGGCTCGCATTGTGGGGG
AGTGGGCACATGAGGCGGTGCAACTAGCGGATTCCATTGGGCTTCTCCTG
TCGATCCTTGCGGGTGTTGTCCTTGTAACACTCCACAAACAGGGGCAATG
GGGA
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