mRNA_C-tenellus_contig6908.1.1 (mRNA) Choristocarpus tenellus KU2346

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_C-tenellus_contig6908.1.1
Unique NamemRNA_C-tenellus_contig6908.1.1
TypemRNA
OrganismChoristocarpus tenellus KU2346 (Choristocarpus tenellus KU2346)
Homology
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BLAST of mRNA_C-tenellus_contig6908.1.1 vs. uniprot
Match: D7FYK6_ECTSI (Aminodeoxychorismate synthase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FYK6_ECTSI)

HSP 1 Score: 159 bits (403), Expect = 3.260e-39
Identity = 83/128 (64.84%), Postives = 97/128 (75.78%), Query Frame = 2 
Query:  380 LNRADLSE-IKRVTDAT---PGLQSVGRERVVTRGFPIELSLGAPRHRPGTDGFVTSGGVEVTCEVMDVDDAARSVEELVEALDTRKGVLLSSSYEFPGRYARWTLGFVDPPLELSGSGRNFRVRALN 751
            LN AD+SE + +V  A    P + +        RGFP  LSLGAPRHRPGTDG+ T G VEV+C+VMDV  A   VE+LV ALD+R+GVLL+SSYEFPGRYARWTLGF DPPLELSG+GR+FRVRALN
Sbjct:    6 LNGADVSEDLTQVNGAAAMPPPVAANTARTAQQRGFPATLSLGAPRHRPGTDGYTTKGNVEVSCKVMDVPHAEECVEDLVHALDSRRGVLLTSSYEFPGRYARWTLGFSDPPLELSGTGRDFRVRALN 133
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BLAST of mRNA_C-tenellus_contig6908.1.1 vs. uniprot
Match: A0A835Z6A5_9STRA (Aminodeoxychorismate synthase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z6A5_9STRA)

HSP 1 Score: 142 bits (357), Expect = 4.250e-33
Identity = 66/98 (67.35%), Postives = 79/98 (80.61%), Query Frame = 2 
Query:  464 TRGFPIELSLGAPRHRPGTDGFVTSGGVEVTCEVMDVDDAARSVEELVEALDTRKGVLLSSSYEFPGRYARWTLGFVDPPLELSGSGRNFRVRALNAR 757
            ++GFP  LSLGAPRHRP  D + T GGVEVTCEV +V DA   VE L+ ALD ++GVLL+SSYEFPGRYARWTLGFV+PP+E++G+G  F VRALN R
Sbjct:   14 SKGFPAGLSLGAPRHRPAQDSYTTGGGVEVTCEVGEVFDATERVEALISALDDQRGVLLTSSYEFPGRYARWTLGFVNPPIEVAGTGDRFTVRALNER 111
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BLAST of mRNA_C-tenellus_contig6908.1.1 vs. uniprot
Match: W7TEM3_9STRA (Aminodeoxychorismate synthase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TEM3_9STRA)

HSP 1 Score: 129 bits (325), Expect = 7.900e-29
Identity = 58/91 (63.74%), Postives = 75/91 (82.42%), Query Frame = 2 
Query:  485 LSLGAPRHRPGTDGFVTSGGVEVTCEVMDVDDAARSVEELVEALDTRKGVLLSSSYEFPGRYARWTLGFVDPPLELSGSGRNFRVRALNAR 757
            LSLGAPR+RP +  F+T GGV+VTC+V DV D  + V+ L+E LD+ +GVLL+SSYEFPGRYARWT+GF+DPPL++   G NF+V+ALNAR
Sbjct:  116 LSLGAPRYRPLSGRFITKGGVQVTCQVEDVSDTGKEVDALLEGLDSHEGVLLASSYEFPGRYARWTIGFIDPPLKIESRGLNFKVKALNAR 206
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BLAST of mRNA_C-tenellus_contig6908.1.1 vs. uniprot
Match: A0A7S2HFX2_9STRA (Aminodeoxychorismate synthase n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2HFX2_9STRA)

HSP 1 Score: 115 bits (289), Expect = 3.440e-24
Identity = 58/120 (48.33%), Postives = 83/120 (69.17%), Query Frame = 2 
Query:  398 SEIKRVTDATPGLQSVGRERVVTRGFPIELSLGAPRHRPGTDGFVTSGGVEVTCEVMDVDDAARSVEELVEALDTRKGVLLSSSYEFPGRYARWTLGFVDPPLELSGSGRNFRVRALNAR 757
            S   RVTDA P   S     V +      L+LGAPR RP +  ++T G V V+  V D+++   ++E+L++ LD ++G L +SSYEFPGRYARWTLGF++PP+E+SG+GR+F+V+ALN R
Sbjct:   47 SATDRVTDAEPLPLSTTYPEVESG----RLTLGAPRDRPRSRKYMTKGSVGVSVAVNDLENPRETIEKLIDTLDMKRGALFTSSYEFPGRYARWTLGFINPPIEISGTGRDFKVKALNER 162
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BLAST of mRNA_C-tenellus_contig6908.1.1 vs. uniprot
Match: A0A7S3ZU32_9STRA (Aminodeoxychorismate synthase n=2 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A7S3ZU32_9STRA)

HSP 1 Score: 111 bits (277), Expect = 1.230e-22
Identity = 57/96 (59.38%), Postives = 71/96 (73.96%), Query Frame = 2 
Query:  476 PIE--LSLGAPRHRPGTDGFVTSGGVEVTCEVMDVDDAARSVEELVEALDTRKGVLLSSSYEFPGRYARWTLGFVDPPLELSGSGRNFRVRALNAR 757
            P+E  LSLGAPR +P    +VT+GGV VT +   V+DA  + E LV+ALD R+GVL+ SSYEFPGRYARWT+GF DPP+ LSG  R+F + ALN R
Sbjct:  119 PLESVLSLGAPRFQPPNGTYVTAGGVGVTRQSFTVEDAHGATEALVDALDDRRGVLMHSSYEFPGRYARWTVGFSDPPVLLSGRARDFALSALNDR 214
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BLAST of mRNA_C-tenellus_contig6908.1.1 vs. uniprot
Match: A0A7S4MER5_9STRA (Aminodeoxychorismate synthase n=3 Tax=Odontella aurita TaxID=265563 RepID=A0A7S4MER5_9STRA)

HSP 1 Score: 110 bits (276), Expect = 1.740e-22
Identity = 67/141 (47.52%), Postives = 83/141 (58.87%), Query Frame = 2 
Query:  398 SEIKRVTDATPGLQSVGRERVVTRG--------FPIELSLGAP-RHRPGTDGFVTSGGVEVTCEVMDVD------------DAARSVEELVEALDTRKGVLLSSSYEFPGRYARWTLGFVDPPLELSGSGRNFRVRALNAR 757
            SE    +  TP   +V   +   RG        F   LSLGAP   RPG   F+T GGV+VT  V +V             ++A  +E+LV+ LDTR+GVLLSSSYEFPGRYARW++GF DPPLE+SG G+   VRALN R
Sbjct:   47 SETAPSSSVTPNYNAVHVAKTGGRGSRTAGQAAFESGLSLGAPIALRPGGGHFLTRGGVQVTAHVEEVQHSNKPIVGGEQTNSASMIEDLVDKLDTRRGVLLSSSYEFPGRYARWSVGFTDPPLEISGRGQKCTVRALNKR 187
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BLAST of mRNA_C-tenellus_contig6908.1.1 vs. uniprot
Match: A0A7R9WEH9_9STRA (Hypothetical protein (Fragment) n=1 Tax=Pseudictyota dubia TaxID=2749911 RepID=A0A7R9WEH9_9STRA)

HSP 1 Score: 108 bits (270), Expect = 1.920e-22
Identity = 60/113 (53.10%), Postives = 73/113 (64.60%), Query Frame = 2 
Query:  485 LSLGAPRHRPGTDGFVTSGGVEVTCEVMDV--------------DD--------AARSVEELVEALDTRKGVLLSSSYEFPGRYARWTLGFVDPPLELSGSGRNFRVRALNAR 757
            LSLGAPR +P    F+T GGV+VT  V  V              DD        +A  VE+LV+ LDTR+GVLL+SSYEFPGRYARW+LGFVDPPLE++G G+   +RALN R
Sbjct:  109 LSLGAPRSQPSGGHFLTKGGVQVTAHVETVPFGTKAAASSEEAKDDKVGKSIRNSASRVEDLVDMLDTRRGVLLTSSYEFPGRYARWSLGFVDPPLEIAGRGQKCTIRALNER 221
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BLAST of mRNA_C-tenellus_contig6908.1.1 vs. uniprot
Match: A0A7S2XWD8_9STRA (Aminodeoxychorismate synthase n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2XWD8_9STRA)

HSP 1 Score: 110 bits (275), Expect = 2.170e-22
Identity = 56/93 (60.22%), Postives = 69/93 (74.19%), Query Frame = 2 
Query:  485 LSLGAPRHRPGTDGFVTSGGVEV-TC-EVMDVDDAARSVEELVEALDTRKGVLLSSSYEFPGRYARWTLGFVDPPLELSGSGRNFRVRALNAR 757
            L+LGAPR++P T  +VT GGVEV  C E +D  +A   V +L + LD  KGVLLSSSY FPGRYARW+LGF  PPLE+ GSGR+FR++ LN R
Sbjct:   73 LTLGAPRYQPDTGSYVTDGGVEVDVCSEELDYLEAQTMVRQLAQTLDDHKGVLLSSSYIFPGRYARWSLGFAKPPLEVVGSGRDFRIKVLNQR 165
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BLAST of mRNA_C-tenellus_contig6908.1.1 vs. uniprot
Match: A0A7S2R582_9STRA (Hypothetical protein (Fragment) n=1 Tax=Eucampia antarctica TaxID=49252 RepID=A0A7S2R582_9STRA)

HSP 1 Score: 108 bits (269), Expect = 6.100e-22
Identity = 66/162 (40.74%), Postives = 95/162 (58.64%), Query Frame = 2 
Query:  338 TARARRAVNSSP--LQLNRADLSEIKRVTDATPGLQSVGRERVVTRG--------FPIELSLGAPRHRPGTDGFVTSGGVEVTCEVMDVD------------DAARSVEELVEALDTRKGVLLSSSYEFPGRYARWTLGFVDPPLELSGSGRNFRVRALNAR 757
            TA ++ A +S+P  +Q ++  LS +   T + P   SV   +   RG           +LSLGAP+ RP    F+T GGV++T  V  ++             +A ++E+LV+ LD RKGVL +SSYE+PGRY RW+LGFVDPPLE+SG G+   + ALN+R
Sbjct:   23 TALSKHAYSSTPFLVQSSKTALSMVS--TSSPPDYNSVHVAKTGGRGAISSSQDALNKDLSLGAPKDRPIGGHFLTRGGVQITANVKALNFVRQGTKDAAEGSSAHAIEQLVDKLDNRKGVLFASSYEYPGRYGRWSLGFVDPPLEVSGKGQQCTITALNSR 182
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BLAST of mRNA_C-tenellus_contig6908.1.1 vs. uniprot
Match: A0A7S1YQY3_9STRA (Aminodeoxychorismate synthase n=3 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S1YQY3_9STRA)

HSP 1 Score: 108 bits (271), Expect = 7.510e-22
Identity = 55/103 (53.40%), Postives = 73/103 (70.87%), Query Frame = 2 
Query:  485 LSLGAPRHRPGTDGFVTSGGVEVTCEVMDV------------DDAARSVEELVEALDTRKGVLLSSSYEFPGRYARWTLGFVDPPLELSGSGRNFRVRALNAR 757
            LSLGAP+ RP    ++T GGV+VT +V  +              +  +VE+LVE LD+++GVLL+SSYEFPGRYARW+LGFVDPPLE+SG+G+   +RALN R
Sbjct:   65 LSLGAPQKRPSGGHYLTRGGVQVTADVKSLRFSSNKDSINVPGTSLHAVEQLVEKLDSQRGVLLTSSYEFPGRYARWSLGFVDPPLEISGTGQKCTIRALNDR 167
The following BLAST results are available for this feature:
BLAST of mRNA_C-tenellus_contig6908.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FYK6_ECTSI3.260e-3964.84Aminodeoxychorismate synthase n=1 Tax=Ectocarpus s... [more]
A0A835Z6A5_9STRA4.250e-3367.35Aminodeoxychorismate synthase n=1 Tax=Tribonema mi... [more]
W7TEM3_9STRA7.900e-2963.74Aminodeoxychorismate synthase n=2 Tax=Monodopsidac... [more]
A0A7S2HFX2_9STRA3.440e-2448.33Aminodeoxychorismate synthase n=1 Tax=Dictyocha sp... [more]
A0A7S3ZU32_9STRA1.230e-2259.38Aminodeoxychorismate synthase n=2 Tax=Pelagomonas ... [more]
A0A7S4MER5_9STRA1.740e-2247.52Aminodeoxychorismate synthase n=3 Tax=Odontella au... [more]
A0A7R9WEH9_9STRA1.920e-2253.10Hypothetical protein (Fragment) n=1 Tax=Pseudictyo... [more]
A0A7S2XWD8_9STRA2.170e-2260.22Aminodeoxychorismate synthase n=1 Tax=Fibrocapsa j... [more]
A0A7S2R582_9STRA6.100e-2240.74Hypothetical protein (Fragment) n=1 Tax=Eucampia a... [more]
A0A7S1YQY3_9STRA7.510e-2253.40Aminodeoxychorismate synthase n=3 Tax=Ditylum brig... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
C-tenellus_contig6908contigC-tenellus_contig6908:2..2314 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Choristocarpus tenellus KU2346 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog2880.D7FYK6
PFAMsAnth_synt_I_N,Chorismate_bind,GATase
Model size1241
Max annot lvl2759|Eukaryota
Hectar predicted targeting categorychloroplast
Exons3
Evalue3.87e-43
EggNOG OGsCOG0147@1|root,COG0512@1|root,KOG0026@2759|Eukaryota,KOG1223@2759|Eukaryota
Descriptionanthranilate synthase activity
Cds size591
COG categoryEH
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680790000.663902-UTR-C-tenellus_contig6908:1..4821680790000.663902-UTR-C-tenellus_contig6908:1..482Choristocarpus tenellus KU2346UTRC-tenellus_contig6908 2..482 -
1680790000.7173064-UTR-C-tenellus_contig6908:2145..23141680790000.7173064-UTR-C-tenellus_contig6908:2145..2314Choristocarpus tenellus KU2346UTRC-tenellus_contig6908 2146..2314 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680790000.6795018-CDS-C-tenellus_contig6908:482..6801680790000.6795018-CDS-C-tenellus_contig6908:482..680Choristocarpus tenellus KU2346CDSC-tenellus_contig6908 483..680 -
1680790000.6888547-CDS-C-tenellus_contig6908:1414..15221680790000.6888547-CDS-C-tenellus_contig6908:1414..1522Choristocarpus tenellus KU2346CDSC-tenellus_contig6908 1415..1522 -
1680790000.7018569-CDS-C-tenellus_contig6908:1860..21451680790000.7018569-CDS-C-tenellus_contig6908:1860..2145Choristocarpus tenellus KU2346CDSC-tenellus_contig6908 1861..2145 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_C-tenellus_contig6908.1.1prot_C-tenellus_contig6908.1.1Choristocarpus tenellus KU2346polypeptideC-tenellus_contig6908 483..2145 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_C-tenellus_contig6908.1.1

>prot_C-tenellus_contig6908.1.1 ID=prot_C-tenellus_contig6908.1.1|Name=mRNA_C-tenellus_contig6908.1.1|organism=Choristocarpus tenellus KU2346|type=polypeptide|length=197bp
MVRSLAVALVVVTYFWTPPAYAFVPVRISLPGSRQLLPGDSLEALSKVAR
NQCYQRTARARRAVNSSPLQLNRADLSEIKRVTDATPGLQSVGRERVVTR
GFPIELSLGAPRHRPGTDGFVTSGGVEVTCEVMDVDDAARSVEELVEALD
TRKGVLLSSSYEFPGRYARWTLGFVDPPLELSGSGRNFRVRALNAR*
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mRNA from alignment at C-tenellus_contig6908:2..2314-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_C-tenellus_contig6908.1.1 ID=mRNA_C-tenellus_contig6908.1.1|Name=mRNA_C-tenellus_contig6908.1.1|organism=Choristocarpus tenellus KU2346|type=mRNA|length=2313bp|location=Sequence derived from alignment at C-tenellus_contig6908:2..2314- (Choristocarpus tenellus KU2346)
CTCCAGGACAGGTTTCTTTGTCGTTGGTAAGTCCTTTTAATTGAATTATT
GCCGAGACTGCTAGAGACCTATACCCACTCCGACACACTTTAATCTCAGA
GAAGAAGTCAGTTGTGGTACTCTACAAGGCCCTACACTCTACCTGAGGTT
GATCTACGTATACCACCTTATGGTTCGTTCATTGGCCGTCGCGTTGGTCG
TGGTGACCTACTTTTGGACACCACCAGCATATGCTTTTGTCCCTGTACGA
ATCTCCCTGCCTGGATCAAGGCAACTTCTGCCGGGTGATAGTCTTGAAGC
TCTTTCTAAGGTAGCTAGGAACCAATGCTACCAGAGGACTGCCAGGGCCC
GCCGTGCAGTGAACTCATCCCCTTTGCAACTCAATCGAGCAGACTTATCT
GAGATTAAAAGAGTGACGGATGCGACGCCTGGACTGCAGTCTGTCGGTAG
GGAGGTAAGGGCACTGGTATGTTTTTGATACTGCTACCAACCTTTTTGCT
CAGGATTAGGTCCTCTTCCAGCCATTATTCAGCCTTACAAGGTAGATGTG
ACAAGTTAACATAAGAGGTTGAGGATGAGTTGACATGTAGTCACCTCGGC
TGTATGTGTGTACCTCTCCTCAAGCCCACACCCAGCTGTAGCCCTCGCTT
TCTTTACAAGTGCCAGATGTTGCCACAGAATAGTCCAGGTGAATTATCAC
TCTCACCTTCGTCACTCGAACGACCAAAACATTGCGAATGATGGCTTTGT
ATGGGTGAACTGCTTCCCCACCTTCCAATTCACACTTGGCAGCGTGTTGT
GACGAGGGGATTCCCGATAGAGTTGTCACTCGGGGCACCTCGACATCGGC
CAGGAACTGATGGTTTCGTCACTAGCGGTGGTGTTGAGGTCACCTGTGAG
GTGAGTTGCTGAGTTGGGGCTGACTGAGGCAGCAATCAGGGATGTCCAGC
TGGTCCCAACTTCCTGTTGGAGCTCTACGACAACACCACAATAACATGCA
TACAATTTCTACACGTTTCTTTCAACGAAACGGTTCAATACAGTCAGAAT
TGTATATATTCAGTATATATTTATCCCAACCCTAGCAAGCATATTGCAGC
ATCTTCTTGAAAGTAAAGGATACACAACGGAATTTCTCTTTATCTTCCCT
TCACCTTTACCTTCTGTCCTCACTCCATACATCCATCTCTTAACCTTCAA
CCTCTCTCCCTCCCCCAACTGTCCCCATTCATCCGCTGACTTTGCCTTAA
ATAACTCTCTCTCTCTCCTTGTCCTCATTCCACACCCTGTCCCCTCCTAC
CCTACCCTTCACCCCCCCCATAGCTTGCCTTACCCTTGTGCCCATCGAGT
GAAAGTATCTTAGGGTTAGGTTACAACTTTTTCCCTGTTTGTCCATTTGG
GGCTTGGCTTCCCTCAGGATTTTTTTTTATTGAATTTTCCTACTCCAAAA
TACTTCTTAGTAGGTAAAACGTACGCATCATGAAAAGGATTTTGACACTT
CCTAACAAACACAATAACCAAAATTACATCAAAATCGCAACATTGGGTTT
TGGCCCCCAAATGGACGCGGCTCTACTCTACCCTCTTAGATAATTTGGCT
CCATCACGTATACTCTCCCTGTACTTCTCTGCAGGTGATGGATGTGGACG
ATGCGGCGAGGAGTGTTGAGGAGCTAGTAGAGGCCTTGGACACAAGAAAG
GGAGTCCTCCTGTCCTCATCGTATGAATTTCCCGGCCGGTATGCTCGCTG
GACTCTGGGGTTTGTAGACCCGCCTCTGGAACTGTCGGGTTCAGGGAGAA
ATTTCCGTGTCCGGGCGCTAAATGCCAGGTGAGATAGATGCGGATTGAGG
CGAACGAGGTGAACAGCCAATGTGCTGTATGGGGTGCAATGTACCCTGTA
TTGAGTGATTGGTTGATGTCTTCCTTTAGGTGTTCAGTTGGGAGATGCGT
GAAGTATGTATGTATGTATGTATATATATAGGTGTTTTAAAGAGTGTAGA
TGGAGTGGTTTTACCTCCTGTCGTATATGGGGGGCATACTTTAGTACCTT
AAGTGCTCTGTGTGAGAGAGGACCTCGGCTATACCCTTTGCCGGGACGAG
ACCTTGCCTTTTTTTTGTGTGAGGGAAGTGTCGTGGGTCCGGTTGAGGTG
TACCTCATTCGTGTACGTTATACCTCATTCGTGTACGTTGTACCTCATTC
GTGTAACGTTGTACCTCATTCGTGTACGTTGTACCTCATTCGTGTACGGT
GTACCTCATTCGTGTACGGTGTACCTCATTCGTGTACGGTGTACCTCATT
CGTGTACGGTGTA
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Coding sequence (CDS) from alignment at C-tenellus_contig6908:2..2314-

>mRNA_C-tenellus_contig6908.1.1 ID=mRNA_C-tenellus_contig6908.1.1|Name=mRNA_C-tenellus_contig6908.1.1|organism=Choristocarpus tenellus KU2346|type=CDS|length=591bp|location=Sequence derived from alignment at C-tenellus_contig6908:2..2314- (Choristocarpus tenellus KU2346)
ATGGTTCGTTCATTGGCCGTCGCGTTGGTCGTGGTGACCTACTTTTGGAC
ACCACCAGCATATGCTTTTGTCCCTGTACGAATCTCCCTGCCTGGATCAA
GGCAACTTCTGCCGGGTGATAGTCTTGAAGCTCTTTCTAAGGTAGCTAGG
AACCAATGCTACCAGAGGACTGCCAGGGCCCGCCGTGCAGTGAACTCATC
CCCTTTGCAACTCAATCGAGCAGACTTATCTGAGATTAAAAGAGTGACGG
ATGCGACGCCTGGACTGCAGTCTGTCGGTAGGGAGCGTGTTGTGACGAGG
GGATTCCCGATAGAGTTGTCACTCGGGGCACCTCGACATCGGCCAGGAAC
TGATGGTTTCGTCACTAGCGGTGGTGTTGAGGTCACCTGTGAGGTGATGG
ATGTGGACGATGCGGCGAGGAGTGTTGAGGAGCTAGTAGAGGCCTTGGAC
ACAAGAAAGGGAGTCCTCCTGTCCTCATCGTATGAATTTCCCGGCCGGTA
TGCTCGCTGGACTCTGGGGTTTGTAGACCCGCCTCTGGAACTGTCGGGTT
CAGGGAGAAATTTCCGTGTCCGGGCGCTAAATGCCAGGTGA
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