mRNA_C-tenellus_contig11792.1.1 (mRNA) Choristocarpus tenellus KU2346

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_C-tenellus_contig11792.1.1
Unique NamemRNA_C-tenellus_contig11792.1.1
TypemRNA
OrganismChoristocarpus tenellus KU2346 (Choristocarpus tenellus KU2346)
Homology
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BLAST of mRNA_C-tenellus_contig11792.1.1 vs. uniprot
Match: D7FMD2_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FMD2_ECTSI)

HSP 1 Score: 102 bits (255), Expect = 9.820e-22
Identity = 91/249 (36.55%), Postives = 140/249 (56.22%), Query Frame = 1 
Query:    1 LAETESEAEPSRRLVGRLESEKASLVMEVDTLRQEVANWKKRVTSLTLSFNAVDPEEHKQVKAEKQQLV-------ETVSSMENKAAEAEKERKMLERDRDNYVREKQRLASKVQTC*QRSAALEKATKETAIRRLEAQLTSEKERLAKALAETATVRAELQRARSTNTATTAMA--ELREQVSKLSAEKAEMTADMD----------------KKSKELE----SKERENVTNRTMYENMKEVGRRMK 660
            L++  ++  P +R   +  +  ASL  E ++LR+EV NWKKRVTSLT SFNAVDP++H+ V AEK++L        +T +++EN  A+  + +   E++RD+    K ++  +V    ++S +   A K  A R LEA+L +E+E+      E A++R E  R R++  +TTA    +L EQV+K  AE   +   +                 KK   LE    +K+RENVTNRTMY+NMK V R++K
Sbjct:  154 LSQLRAQMAPLQRAQRQQSASSASLASEKESLRKEVENWKKRVTSLTASFNAVDPKDHEDVIAEKERLAKALAEADQTKAALENAKADLTRGKAAAEKERDDANAIKAKVLPQVHEL-RKSLSASAAQKAAASRDLEAKLAAEREKXXXXXXEAASLRQENARTRASANSTTANVTKQLNEQVAKAKAEGEALRVQLRQALASKDGSGQSEMLRKKVASLEEAIRAKDRENVTNRTMYDNMKGVSRKIK 401
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BLAST of mRNA_C-tenellus_contig11792.1.1 vs. uniprot
Match: A0A6H5KRL3_9PHAE (TPR_MLP1_2 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KRL3_9PHAE)

HSP 1 Score: 95.1 bits (235), Expect = 6.860e-19
Identity = 91/253 (35.97%), Postives = 138/253 (54.55%), Query Frame = 1 
Query:    1 LAETESEAEPSRRLVGRLESEKASLVMEVDTLRQEVANWKKRVTSLTLSFNAVDPEEHKQVKAEKQQLVETVSSMENKAAEAEKERKML-------ERDRDNYVREKQRLASKVQTC*QRSAALEKATKETAIRRLEAQLTSEKERLAKALAETATVRAELQRARSTNTATTAMA--ELREQ----VSKLSAEKAEMTADMD----------------KKSKELE----SKERENVTNRTMYENMKEVGRRMK 660
            L++  ++  P +R   +  +  ASL  E ++LR+EV +WKKRVT+LT SFNAVDP++H+ V AEK++L + ++  E   A  E     L       E++RD+    K +L  +V    ++S +   A K    R LEA+L +E+E+ +KA AE A++R E  R R++  + TA    +L EQ    V+K  AE   +   +                 KK   LE    +K+RENVTNRTMY+NMK V R++K
Sbjct:  856 LSQLRAQMAPLQRAQRQQSASSASLASEKESLRKEVESWKKRVTNLTASFNAVDPKDHEDVIAEKERLAKALAEAEQAKAALENAXXXLTRGKAAAEKERDDANAIKAKLLPQVHEL-RKSLSASAAQKAATSRDLEAKLAAEREKASKAAAEAASLRQENARTRASANSNTANVTKQLNEQATLVVAKAKAEGEALRVQLRQALASKDGSGQSEMLRKKVASLEEAIQAKDRENVTNRTMYDNMKGVSRKIK 1107
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BLAST of mRNA_C-tenellus_contig11792.1.1 vs. uniprot
Match: A0A448ZKX7_9STRA (TPR_MLP1_2 domain-containing protein n=1 Tax=Pseudo-nitzschia multistriata TaxID=183589 RepID=A0A448ZKX7_9STRA)

HSP 1 Score: 62.8 bits (151), Expect = 8.330e-8
Identity = 37/89 (41.57%), Postives = 57/89 (64.04%), Query Frame = 1 
Query:    1 LAETESEAEPSRRLVGRLESEKASLVMEVDTLRQEVANWKKRVTSLTLSFNAVDPEEHKQVKAEKQQLVETVSSMENKAAEAEKERKML 267
            + E++   +PS     +LES+KA L+ E D+L +E+ +WK RV SL   FN VDPEEH +V  + ++L + V S+E K + AE+E K +
Sbjct: 1309 VEESKKALQPSADKQKQLESDKAVLLAEKDSLLREIDDWKGRVQSLVSKFNQVDPEEHLKVVKKSEELEKQVKSLEEKKSYAEEESKRI 1397
The following BLAST results are available for this feature:
BLAST of mRNA_C-tenellus_contig11792.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 3
Match NameE-valueIdentityDescription
D7FMD2_ECTSI9.820e-2236.55Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5KRL3_9PHAE6.860e-1935.97TPR_MLP1_2 domain-containing protein n=1 Tax=Ectoc... [more]
A0A448ZKX7_9STRA8.330e-841.57TPR_MLP1_2 domain-containing protein n=1 Tax=Pseud... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
C-tenellus_contig11792contigC-tenellus_contig11792:245..4946 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Choristocarpus tenellus KU2346 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog2880.D7FMD2
PFAMsTPR_MLP1_2
Model size660
Max annot lvl2759|Eukaryota
KEGG koko:K09291,ko:K10405,ko:K12472,ko:K20283
KEGG TC1.I.1
KEGG Pathwayko03013,ko04144,ko05200,ko05216,map03013,map04144,map05200,map05216
KEGG ModuleM00427
Hectar predicted targeting categoryother localisation
GOsGO:0000003,GO:0000075,GO:0000278,GO:0003006,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005635,GO:0005643,GO:0005730,GO:0005737,GO:0005829,GO:0005911,GO:0006403,GO:0006405,GO:0006406,GO:0006611,GO:0006810,GO:0006886,GO:0006913,GO:0007049,GO:0007088,GO:0007093,GO:0007094,GO:0007275,GO:0007346,GO:0008104,GO:0008150,GO:0008152,GO:0009506,GO:0009507,GO:0009536,GO:0009791,GO:0009892,GO:0009908,GO:0009909,GO:0009910,GO:0009987,GO:0010467,GO:0010468,GO:0010564,GO:0010605,GO:0010639,GO:0010948,GO:0010965,GO:0012505,GO:0015031,GO:0015833,GO:0015931,GO:0016973,GO:0019222,GO:0022402,GO:0022414,GO:0030054,GO:0030071,GO:0031323,GO:0031324,GO:0031399,GO:0031400,GO:0031503,GO:0031577,GO:0031967,GO:0031974,GO:0031975,GO:0031981,GO:0032268,GO:0032269,GO:0032501,GO:0032502,GO:0032991,GO:0033036,GO:0033043,GO:0033044,GO:0033045,GO:0033046,GO:0033047,GO:0033048,GO:0033233,GO:0033234,GO:0034613,GO:0042886,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0044422,GO:0044424,GO:0044428,GO:0044444,GO:0044446,GO:0044464,GO:0045184,GO:0045786,GO:0045839,GO:0045841,GO:0045930,GO:0046907,GO:0048367,GO:0048437,GO:0048438,GO:0048443,GO:0048466,GO:0048519,GO:0048523,GO:0048580,GO:0048581,GO:0048608,GO:0048731,GO:0048827,GO:0048831,GO:0048856,GO:0050657,GO:0050658,GO:0050789,GO:0050793,GO:0050794,GO:0051028,GO:0051093,GO:0051128,GO:0051129,GO:0051168,GO:0051169,GO:0051171,GO:0051172,GO:0051179,GO:0051234,GO:0051236,GO:0051239,GO:0051241,GO:0051246,GO:0051248,GO:0051641,GO:0051649,GO:0051726,GO:0051783,GO:0051784,GO:0051983,GO:0051985,GO:0055044,GO:0060255,GO:0060968,GO:0061458,GO:0065007,GO:0070013,GO:0070727,GO:0071166,GO:0071173,GO:0071174,GO:0071426,GO:0071427,GO:0071702,GO:0071704,GO:0071705,GO:0080090,GO:0090567,GO:0099402,GO:1901987,GO:1901988,GO:1901990,GO:1901991,GO:1902099,GO:1902100,GO:1903047,GO:1903320,GO:1903321,GO:1905818,GO:1905819,GO:2000026,GO:2000241,GO:2000242,GO:2000816,GO:2001251
Exons6
Evalue1.23e-10
EggNOG OGsKOG4674@1|root,KOG4674@2759|Eukaryota
Descriptionspindle assembly checkpoint
Cds size255
COG categoryS
BRITEko00000,ko00001,ko00002,ko03019,ko03036,ko04131,ko04812
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_C-tenellus_contig11792.1.1prot_C-tenellus_contig11792.1.1Choristocarpus tenellus KU2346polypeptideC-tenellus_contig11792 2879..4868 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680789195.245633-UTR-C-tenellus_contig11792:244..3851680789195.245633-UTR-C-tenellus_contig11792:244..385Choristocarpus tenellus KU2346UTRC-tenellus_contig11792 245..385 -
1680789195.2645056-UTR-C-tenellus_contig11792:696..7891680789195.2645056-UTR-C-tenellus_contig11792:696..789Choristocarpus tenellus KU2346UTRC-tenellus_contig11792 697..789 -
1680789195.2723544-UTR-C-tenellus_contig11792:2162..22071680789195.2723544-UTR-C-tenellus_contig11792:2162..2207Choristocarpus tenellus KU2346UTRC-tenellus_contig11792 2163..2207 -
1680789195.2798622-UTR-C-tenellus_contig11792:2830..28781680789195.2798622-UTR-C-tenellus_contig11792:2830..2878Choristocarpus tenellus KU2346UTRC-tenellus_contig11792 2831..2878 -
1680789195.3246167-UTR-C-tenellus_contig11792:4868..49461680789195.3246167-UTR-C-tenellus_contig11792:4868..4946Choristocarpus tenellus KU2346UTRC-tenellus_contig11792 4869..4946 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680789195.2877889-CDS-C-tenellus_contig11792:2878..28801680789195.2877889-CDS-C-tenellus_contig11792:2878..2880Choristocarpus tenellus KU2346CDSC-tenellus_contig11792 2879..2880 -
1680789195.3009725-CDS-C-tenellus_contig11792:3518..36931680789195.3009725-CDS-C-tenellus_contig11792:3518..3693Choristocarpus tenellus KU2346CDSC-tenellus_contig11792 3519..3693 -
1680789195.314227-CDS-C-tenellus_contig11792:4790..48681680789195.314227-CDS-C-tenellus_contig11792:4790..4868Choristocarpus tenellus KU2346CDSC-tenellus_contig11792 4791..4868 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_C-tenellus_contig11792.1.1

>prot_C-tenellus_contig11792.1.1 ID=prot_C-tenellus_contig11792.1.1|Name=mRNA_C-tenellus_contig11792.1.1|organism=Choristocarpus tenellus KU2346|type=polypeptide|length=85bp
MEVDTLRQEVANWKKRVTSLTLSFNAVDPEEHKQVKAEKQQLVETVSSME
NKAAEAEKERKMLERDRDNYVREKQRLASKVQTC*
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mRNA from alignment at C-tenellus_contig11792:245..4946-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_C-tenellus_contig11792.1.1 ID=mRNA_C-tenellus_contig11792.1.1|Name=mRNA_C-tenellus_contig11792.1.1|organism=Choristocarpus tenellus KU2346|type=mRNA|length=4702bp|location=Sequence derived from alignment at C-tenellus_contig11792:245..4946- (Choristocarpus tenellus KU2346)
CTGGCAGAGACAGAGTCAGAAGCGGAGCCCTCCCGACGCCTAGTGGGCCG
ACTGGAGTCTGAGAAGGCATCACTGGTGATGGAAGTGGACACCTTGAGGC
AGGAAGTCGCAAACTGGAAGAAGAGGGTCACCAGCCTAACCCTCAGCTTT
AATGCGGTGAGGGGTATGGTTTGATATTTTTATTGTTATTATTATCATTA
TTTTTATTGTTTGTAGTTTTGTCATGGCGTGTCCATCTTCAACCATCATT
GTTACTACTTTTATTACTATTTTTATTATTGTTTTTTTTTAATGTTGCTG
TAAATGTCTTCTGTGTAGGATATGATTTGCTGCAAATGACTTGATTTTGG
TCATTGTGGTTTCTGATATCAATGACATATGTGTTTATGTTTGTGTGTGT
GTGTGTGTTTCACAGTATTGTAGTATGATGATAGATGTTTTGAAGGAGAT
ATTTGTAGTAGAAGGTAAAGGTAAGGAAGCTGTTTTTCCATCTGATAAAT
TGTGTACTTTGTGTTCGAAGTATTTTCAATGCTGTTTTGACAGTTACTGT
AGCGATACTGAAGACAGTTACTGTAGTGATACTGAAGACAGTGACTGTAG
CGATACTGAAGACAGTTACTGTAGTGATACTGAAGACAGTTACTGTAGCG
ATACTGAAGACAGTTACTGTAGTGATACTGAAGACAGTGACTGTAGCGAT
ACTGAAGACAGTGACTGTAGTGATACTGAAGACAGTGACTGTAGCGATAC
TGAAGACAGTGACTGTAGTGATACTGAAGACAGTGACTGTAGCGATACTG
AAGACAGTTACTCCTGCATTGTTAACCAAATTTTGTCAATTTTTTTTATG
CTTCCAAAGGTTAATAGTGTTTTGAGTCTTTTGTCGCTACGCTTGTTATT
TATTTTTATTTCCCTAAAAAAAATGAAAGTTATTTATGGATGCTTTTCTT
GTTACCTAGTGATGGGGAAGATTGTGGTTTTTAGGGTTAGGGTAATTGTG
ACATAAACGCCTTTAAAATACGTCATCAGTTATTTTTAAAGGTGATCATG
TGATGTACAATCGATTATTTTTATTATGTAGTATCTTTTTTTCCTTTTTT
TAGAAAGTATTGCAAAAAAGAGGTAACTATTTAACTATTTTTAGAAGGAT
GACAGTTTCAGTGTGATTGTTGAAAACAGAAAACAGATTGTGATAATTAA
TAAAACAATGTACATTTATTTTTACGGCAATAATAATAATAATAATAATT
AAGGTGGATCCGGAGGAGCACAAACAAGTTAAGGCTGAGAAGCAGCAGCT
GGTAGAGACGGTGTCGTCCATGGAGAACAAGGCAGCCGAGGCTGAGAAGG
AAAGGAAGATGCTGGAGAGGGATAGGGATAACTATGTTCGAGAAAAGCAA
CGCCTTGCATCTAAGGTACAAACCTGCTGTACTACTATTGATTGTTAAAC
CGTGCACATTTTGCATTGTGATGGGGGAGGGAGGGGGGGGGATCAGTGGT
TAATGGTTGAGGTGTAGGGTTGGCTCTATGGTGGTACTAGATTTTTTTGG
TGGGTAGGGTAGATGATCATGGGATAAGGTGGGGGAAGAGGTTAGGTTTA
GGGTTTAGTGGGGTAGTGTGGAATATGTGGGGTTTGGGTCAGTCAATGGA
TTTTCGGGGGGTGGGGAGTGGGGTTTTGTAGTCAGGAGTTTCGGAGGTTT
TGGTCCATAATTGTGTTTCTGGATACATGATTTAGTGTTGGCTATTTTTA
TTTTTTTAATAGGGGGTGATGGTGATGTGATGTTTTCGTGGTTCTCTTGT
ATACATAATAATATTGCCATTAAGAGTATGACTTATACTAGTCATATTAT
TATATTGAACATAATGGGAGTGTTACATGGTACAAGTTGTGTCATTTTAG
TAGTAATTTTAGTAATAGTTACAAATGCTGTTGGTAGCTCAGTGGTTTGT
CTCAACACCCAGGTTTTGCTTACATTCTTATTGTTGTTATTGTTATTATT
ATTGTTATTATTATTATTATTGATGTTGTCTCTCTTTGCAGGTGTCTGAT
ATGACCCAGGCAGCAGAGCAGCGCAGTGCAGCATTGGAAAAGGCCACGAA
GGAGACTGCCATTAGGGTCAGTTACTAATATTATTATTTTATCATTATTA
TTTTTGGAGGGGTTAGGGTTATAGGGTATGTGATGTCTTCACAATTCAGT
TAATTGAGGACTTGCCGTGTTTACCCTGTGTGTGTTGTCTGTGTTTTGTC
TGTGTGTGTGTGTGTGTGTTTTGCCTTAGACTTTGCTCCCTGTGCATCAT
GGTTAAGTTTCTGATATAAAAAGTTAATTTTGTTTTGAACGTTCCTTCTT
GGCTTTTTATAGTTATTTTTATAGTCCCATTACTCTCAGTGTTACCGGTA
TGTAACACAAGCAATACGTGTAACTTCATGTGCAGTAATGAAATATGATG
TTACGACAGTAATATGATGGTATGGCATGCTGTGAATTGTTTTACAGTGA
GGACAGATTACCAATCAACAGTTTTCACGCCTAGTTTGACATCAAGCAGC
AAAACACCTGCACTTATTGAAAACTGTACAAAAAATAAAAATAAAATTCA
TTTGTTTTGAAAAAAAAGTATTATCAATAATTTGTTTGTTAGTCATCAGC
GATGGCAGTGTCTCTTTTAAACGTTCCATTCCTAATGTGTTGTGTTGTGT
TATGTTATGTTATGTTATTTGCAGGCACAGTCATTGAAGCGACTTGAGGC
TCAGCTGACTTCTGAGAAGGAGAGGCTTGCTAAGGTCAGTTGAAAGAGAT
AGTTATTGTTCATTTGAATGGACCAGTTACTTCGTTACTTAGGTTACTTA
GTTTTCAAATAGTGGGATAGAACAGCCATGAAAGTCAAGTTGTAATAGGG
AGCATTGTTGACATGACCAGGTAGTCTGTTGAGTGAGTGAAAGTATCTTA
GGGTTAGGGTTAGGGTATGAGTTTTGCCCTGTTCTTGTCCGTGTGGGTCT
TACATATCCTTAGCTTCCCATGTTTTTTTCATGACATTTGCCTACTCCAA
AAATGTTTGGGAGTAGGTAAAATGTATACGTTATGAAGAGGTGTGTCCAG
AACAAGGATATTGACACTTTTTAATGATATGATAGCCAAAATTACATCAA
AATCGCAACATTGGAATTTGGCCCCCAAATGGACACAGCTCTACCCTCTA
AGATACTTTGGTTCAATGGTTAAATTGAGGATACATAAGGTTAATTTAAT
TTCTGTGAGTTAGGGTTGTGGTTGAGATAAGGTCTTGTCCTGTATGGTGC
TAGACAAAAACATAACTATGATATAGTGTTGTATATTTTATGAGGAAAAG
GATAATGATACAATATTATTGGATGAATAGGGTTAGGGTTGGCTTTAACT
CTTTGTTTTTTTGTGTTATTCCTGTTAATAATTAATTTTAAAAAGTGTTG
GTTTGAGTATTGCACTGTGTAGGTTGATGTTAACATACTGATGTTTGACT
GGGGTTAGGGTTTGTGTTTGGTGAATGAAGATGAAGGGATAGACTTGGGT
AGATTAGGTAGATTGAAGGTAAGGTGTTCAACATGTTCCAAACGACTTGA
GTTTTCATCAGTTTTCGATAGGACGAGAAGCCGGTGAATGAAATGATTGA
TTTATGTGTACGTTGGAGTGTTTATATTTTTTGATTAAAAAAAAAGAAAA
AAATTAATGGTAAAAAACAAGAGGAGTTTTATGTTATTATTTTATGTGAG
ATGACAACATTAGATGCAATGGTGTGGGGGAAGCAGGTGTTTTTGTTTGA
CCTGTCTAGTGTTTTGTTGTTATAGCGTGAAGATTTTGGTGTTTGTAGTT
GCTGGGGTGGCATATTGGCATGTTGACATCGGCATCGACGTGTATGTGAT
CAATTTGAAGAATACTAGTGGAGTGTAACTGTAGCATTTTTTTTCTATTT
TTTTGTTACTGTGTAGAAGATGTTAGTGTGCTGTTTTGATTGGTTGAACT
CCACTGAGCTCTTCTACTATGATTTTCTAGAAACATTGTCGAAGGCCTCT
GATTAATCCATGGTTGACAGACATGTGAAGTAAGTAATACTCTCTTTTTT
TTACCTCCTTCCTGTCAACACATTCACACTTAACACTTGGATGACGTTTT
ATGGCAGGCGTTGGCAGAGACTGCCACTGTCAGGGCTGAACTGCAAAGGG
CAAGGTCAACCAACACTGCTACCACTGCCATGGCAGAGCTCAGAGAGCAG
GTGTGTTATTAGTGGGATATGAGGGTGGTGATCCATACTTTCATGTTATG
ATTTTGAAGTAGTAGGTGTTTTTTTGTTTTATGTGGGGTTACTTTTGTGT
TGGTTACATGTGTGTGTATGTATTTATATTCTGGTTGAATGTGCACCTAT
GTATGTACACTTTTTTCTATGATTTTTTTCCATGTAGTATCTATGTCCCC
GTTGGTACTGACTCCCTGCACTGTAGTAGATTGTGCTGATTTGTTTGTTG
TGCATATATATTTTTTAATGTTGGGAATTAAAATTAAAAACAAAAAATAA
ATAAAAACAAGGTGAGCAAGCTGTCGGCGGAGAAGGCAGAAATGACTGCA
GATATGGACAAAAAGTCTAAGGAGCTGGAGAGCAAGGAGAGGGAGAATGT
CACCAACAGAACCATGTATGAAAACATGAAGGAGGTCGGTCGCCGTATGA
AG
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Coding sequence (CDS) from alignment at C-tenellus_contig11792:245..4946-

>mRNA_C-tenellus_contig11792.1.1 ID=mRNA_C-tenellus_contig11792.1.1|Name=mRNA_C-tenellus_contig11792.1.1|organism=Choristocarpus tenellus KU2346|type=CDS|length=255bp|location=Sequence derived from alignment at C-tenellus_contig11792:245..4946- (Choristocarpus tenellus KU2346)
ATGGAAGTGGACACCTTGAGGCAGGAAGTCGCAAACTGGAAGAAGAGGGT
CACCAGCCTAACCCTCAGCTTTAATGCGGTGGATCCGGAGGAGCACAAAC
AAGTTAAGGCTGAGAAGCAGCAGCTGGTAGAGACGGTGTCGTCCATGGAG
AACAAGGCAGCCGAGGCTGAGAAGGAAAGGAAGATGCTGGAGAGGGATAG
GGATAACTATGTTCGAGAAAAGCAACGCCTTGCATCTAAGGTACAAACCT
GCTAG
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