mRNA_C-tenellus_contig10375.2.2 (mRNA) Choristocarpus tenellus KU2346

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_C-tenellus_contig10375.2.2
Unique NamemRNA_C-tenellus_contig10375.2.2
TypemRNA
OrganismChoristocarpus tenellus KU2346 (Choristocarpus tenellus KU2346)
Homology
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BLAST of mRNA_C-tenellus_contig10375.2.2 vs. uniprot
Match: D7FY72_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FY72_ECTSI)

HSP 1 Score: 121 bits (303), Expect = 1.990e-25
Identity = 68/109 (62.39%), Postives = 70/109 (64.22%), Query Frame = 1 
Query: 1030 QIPRTADFILKGGFRRVALQFPDALLPEAPEVLWALQGMLRKAIGEGMRLGGGDVGDAGVE-----------GLEGLPLIFVTGDTSYGSCCVDEVSAQHLKADAIVHY 1323
            +IPRT  FI  G FRRVALQFPD LL EAPEVLW LQ  LRK    G+R     V   G E                PLIFVTGDTSYGSCCVDEVSAQHLKADAIVHY
Sbjct:   53 EIPRTTAFIADGNFRRVALQFPDVLLREAPEVLWTLQARLRKLAAGGVR----SVSQVGREEEGEGKGPSEVATTAPPLIFVTGDTSYGSCCVDEVSAQHLKADAIVHY 157
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BLAST of mRNA_C-tenellus_contig10375.2.2 vs. uniprot
Match: A0A5D6Y0U0_9STRA (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6Y0U0_9STRA)

HSP 1 Score: 93.2 bits (230), Expect = 1.540e-16
Identity = 51/98 (52.04%), Postives = 62/98 (63.27%), Query Frame = 1 
Query: 1030 QIPRTADFILKGGFRRVALQFPDALLPEAPEVLWALQGMLRKAIGEGMRLGGGDVGDAGVEGLEGLPLIFVTGDTSYGSCCVDEVSAQHLKADAIVHY 1323
            +I RTA+ +  GGFR VALQFPD+LLP+AP V    Q  L  A+GE   +             +G   +FV GDTSYGSCCVDEV+AQHL AD +VHY
Sbjct:   40 EIARTAEQLRVGGFRAVALQFPDSLLPDAPRV----QRELTAALGERFAV------------ADGSARVFVLGDTSYGSCCVDEVAAQHLAADCVVHY 121
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BLAST of mRNA_C-tenellus_contig10375.2.2 vs. uniprot
Match: A0A059DB33_EUCGR (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 n=6 Tax=Myrtoideae TaxID=1699513 RepID=A0A059DB33_EUCGR)

HSP 1 Score: 89.4 bits (220), Expect = 2.550e-15
Identity = 54/113 (47.79%), Postives = 68/113 (60.18%), Query Frame = 1 
Query: 1030 QIPRTADFILKGGFRRVALQFPDALLPEAPEVLWALQ---GMLRKAIGEGMRLGGGDVGDAGVEGLEGLPLIFVTGDTSYGSCCVDEVSAQHLKADAIVHYAHLRSKTSYTPT 1359
            +I RTA+FIL   F RVALQFPD LL +A +V+ AL      LR + GE +R G GD+G            +FV  DT++GSCCVDEV A H+ AD ++HY H    T  TPT
Sbjct:    8 EIDRTAEFILSRNFARVALQFPDDLLKDATKVVRALHTKLASLRASSGEEIR-GDGDIG------------LFVMADTTFGSCCVDEVGASHINADCVIHYGH----TCLTPT 103
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BLAST of mRNA_C-tenellus_contig10375.2.2 vs. uniprot
Match: A0A316VDQ5_9BASI (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 n=1 Tax=Meira miltonrushii TaxID=1280837 RepID=A0A316VDQ5_9BASI)

HSP 1 Score: 89.4 bits (220), Expect = 3.010e-15
Identity = 43/102 (42.16%), Postives = 62/102 (60.78%), Query Frame = 1 
Query: 1024 LSQIPRTADFILKGGFRRVALQFPDALLPEAPEVLWALQGMLRKAIGEGMRLGGGDVGDAGVEGLEGLPLIFVTGDTSYGSCCVDEVSAQHLKADAIVHYAH 1329
            L  I   A  IL+  F+R+ALQFPD LL +A +V W+L+  L+++                 +GL  LP ++V  DTSYG+CCVDEV+A+H+ AD +VH+ H
Sbjct:   38 LYDIKHVAQLILQHNFQRIALQFPDELLSDASKVFWSLEDTLKRS-----------------KGLRDLPELYVLADTSYGNCCVDEVAAEHIDADLVVHFGH 122
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BLAST of mRNA_C-tenellus_contig10375.2.2 vs. uniprot
Match: A0A175YLY9_DAUCS (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 n=1 Tax=Daucus carota subsp. sativus TaxID=79200 RepID=A0A175YLY9_DAUCS)

HSP 1 Score: 88.2 bits (217), Expect = 6.260e-15
Identity = 55/118 (46.61%), Postives = 70/118 (59.32%), Query Frame = 1 
Query: 1021 GLSQIPRTADFILKGGFRRVALQFPDALLPEAPEVLWALQG---MLRKAIGEGMRLGGGDVGDAGVEGLEGLPLIFVTGDTSYGSCCVDEVSAQHLKADAIVHYAHLRSKTSYTPTGN 1365
            G  +I   A FIL   FRRVALQFPD LL E+  V+ AL+    +L+K+ GE  R    DV D G         ++V GDT+YGSCCVDEV+A H+ AD ++HY H    T  +PT N
Sbjct:    5 GFYEIEAVARFILTRDFRRVALQFPDELLKESTRVVRALREKLHVLKKSTGERDR----DVRDIG---------LYVMGDTTYGSCCVDEVAASHIHADCVIHYGH----TCLSPTSN 105
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BLAST of mRNA_C-tenellus_contig10375.2.2 vs. uniprot
Match: UPI0003599D9F (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 n=1 Tax=Aplysia californica TaxID=6500 RepID=UPI0003599D9F)

HSP 1 Score: 87.4 bits (215), Expect = 1.060e-14
Identity = 46/100 (46.00%), Postives = 56/100 (56.00%), Query Frame = 1 
Query: 1030 QIPRTADFILKGGFRRVALQFPDALLPEAPEVLWALQGMLRKAIGEGMRLGGGDVGDAGVEGLEGLPLIFVTGDTSYGSCCVDEVSAQHLKADAIVHYAH 1329
            +I R+  +ILKG F+RVALQFPD LL +AP V   +     +A                        L+F+ GDTSYGSCCVDEV AQH KAD I+HY H
Sbjct:   33 EISRSVQWILKGDFKRVALQFPDELLVDAPAVATKIHSEASEA------------------------LVFLLGDTSYGSCCVDEVGAQHYKADCIIHYGH 108
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BLAST of mRNA_C-tenellus_contig10375.2.2 vs. uniprot
Match: A0A8K1C3Y9_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1C3Y9_PYTOL)

HSP 1 Score: 87.0 bits (214), Expect = 1.480e-14
Identity = 48/97 (49.48%), Postives = 58/97 (59.79%), Query Frame = 1 
Query: 1033 IPRTADFILKGGFRRVALQFPDALLPEAPEVLWALQGMLRKAIGEGMRLGGGDVGDAGVEGLEGLPLIFVTGDTSYGSCCVDEVSAQHLKADAIVHY 1323
            I RT + + +GG+R +ALQFPD+LLP+AP V   LQ  L                D   E       +FV GDTSYGSCCVDEV+AQHL+AD IVHY
Sbjct:   41 IVRTVEQLRRGGYRSIALQFPDSLLPDAPRVQVELQSRL----------------DGQTE------RVFVLGDTSYGSCCVDEVAAQHLRADCIVHY 115
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BLAST of mRNA_C-tenellus_contig10375.2.2 vs. uniprot
Match: K3WI16_GLOUD (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3WI16_GLOUD)

HSP 1 Score: 87.0 bits (214), Expect = 1.490e-14
Identity = 46/98 (46.94%), Postives = 57/98 (58.16%), Query Frame = 1 
Query: 1030 QIPRTADFILKGGFRRVALQFPDALLPEAPEVLWALQGMLRKAIGEGMRLGGGDVGDAGVEGLEGLPLIFVTGDTSYGSCCVDEVSAQHLKADAIVHY 1323
            +I RT + I +GG++ +ALQFPD+LLP+AP V   L+  L                        G   IF+ GDTSYGSCCVDEV+AQHL AD IVHY
Sbjct:   40 EIARTLEQIRRGGYKNIALQFPDSLLPDAPRVQQELKNGLESC---------------------GYERIFILGDTSYGSCCVDEVAAQHLFADCIVHY 116
[Close]
BLAST of mRNA_C-tenellus_contig10375.2.2 vs. uniprot
Match: A0A0K9QUC0_SPIOL (Uncharacterized protein (Fragment) n=3 Tax=Spinacia oleracea TaxID=3562 RepID=A0A0K9QUC0_SPIOL)

HSP 1 Score: 86.3 bits (212), Expect = 1.780e-14
Identity = 46/100 (46.00%), Postives = 64/100 (64.00%), Query Frame = 1 
Query: 1030 QIPRTADFILKGGFRRVALQFPDALLPEAPEVLWALQGMLRKAIGEGMRLGGGDVGDAGVEGLEGLPLIFVTGDTSYGSCCVDEVSAQHLKADAIVHYAH 1329
            ++ RTAD+I    F RVALQFPD LL ++  V+ AL+  LR      ++    D G++ VEG + + L +V  DT+YGSCC+DEV A H+ AD +VHY H
Sbjct:    8 EVDRTADYIYSHNFTRVALQFPDELLKDSIRVVRALRDKLRA-----LQKCNDDSGESLVEGRKDVGL-YVMADTTYGSCCIDEVGAAHINADCVVHYGH 101
[Close]
BLAST of mRNA_C-tenellus_contig10375.2.2 vs. uniprot
Match: A0A484E0M7_BRELC (2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 n=1 Tax=Bremia lactucae TaxID=4779 RepID=A0A484E0M7_BRELC)

HSP 1 Score: 86.3 bits (212), Expect = 2.610e-14
Identity = 48/97 (49.48%), Postives = 56/97 (57.73%), Query Frame = 1 
Query: 1033 IPRTADFILKGGFRRVALQFPDALLPEAPEVLWALQGMLRKAIGEGMRLGGGDVGDAGVEGLEGLPLIFVTGDTSYGSCCVDEVSAQHLKADAIVHY 1323
            I RT D I  GG+ ++ALQFPD+LLP+APEV   L+  L                      L     +FV GDTSYGSCCVDEV+AQHL AD IVHY
Sbjct:   41 IARTVDQIRWGGYTKIALQFPDSLLPDAPEVQQELKNEL----------------------LGQYERVFVLGDTSYGSCCVDEVAAQHLLADCIVHY 115
The following BLAST results are available for this feature:
BLAST of mRNA_C-tenellus_contig10375.2.2 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FY72_ECTSI1.990e-2562.39Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
A0A5D6Y0U0_9STRA1.540e-1652.042-(3-amino-3-carboxypropyl)histidine synthase subu... [more]
A0A059DB33_EUCGR2.550e-1547.792-(3-amino-3-carboxypropyl)histidine synthase subu... [more]
A0A316VDQ5_9BASI3.010e-1542.162-(3-amino-3-carboxypropyl)histidine synthase subu... [more]
A0A175YLY9_DAUCS6.260e-1546.612-(3-amino-3-carboxypropyl)histidine synthase subu... [more]
UPI0003599D9F1.060e-1446.002-(3-amino-3-carboxypropyl)histidine synthase subu... [more]
A0A8K1C3Y9_PYTOL1.480e-1449.48Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
K3WI16_GLOUD1.490e-1446.942-(3-amino-3-carboxypropyl)histidine synthase subu... [more]
A0A0K9QUC0_SPIOL1.780e-1446.00Uncharacterized protein (Fragment) n=3 Tax=Spinaci... [more]
A0A484E0M7_BRELC2.610e-1449.482-(3-amino-3-carboxypropyl)histidine synthase subu... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
C-tenellus_contig10375contigC-tenellus_contig10375:2731..5368 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Choristocarpus tenellus KU2346 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start1
Seed ortholog2880.D7FY72
Preferred nameDPH2
PFAMsDiphthamide_syn
Model size1490
Max annot lvl2759|Eukaryota
KEGG koko:K17866
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0006417,GO:0006448,GO:0006464,GO:0006807,GO:0008150,GO:0008152,GO:0009058,GO:0009889,GO:0009987,GO:0010468,GO:0010556,GO:0010608,GO:0016740,GO:0016765,GO:0017182,GO:0017183,GO:0018193,GO:0018202,GO:0019222,GO:0019538,GO:0031323,GO:0031326,GO:0032268,GO:0034248,GO:0036211,GO:0043170,GO:0043412,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044424,GO:0044444,GO:0044464,GO:0050789,GO:0050794,GO:0051171,GO:0051246,GO:0060255,GO:0065007,GO:0071704,GO:0080090,GO:0090560,GO:1900247,GO:1901564,GO:2000112,GO:2000765
Exons2
Evalue1.14e-29
EggNOG OGsCOG1736@1|root,KOG2648@2759|Eukaryota
DescriptionRequired for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2
Cds size477
COG categoryJ
BRITEko00000,ko03012
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680789128.4886286-UTR-C-tenellus_contig10375:2730..37411680789128.4886286-UTR-C-tenellus_contig10375:2730..3741Choristocarpus tenellus KU2346UTRC-tenellus_contig10375 2731..3741 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680789128.5060608-CDS-C-tenellus_contig10375:3741..40541680789128.5060608-CDS-C-tenellus_contig10375:3741..4054Choristocarpus tenellus KU2346CDSC-tenellus_contig10375 3742..4054 +
1680789128.5154579-CDS-C-tenellus_contig10375:5204..53681680789128.5154579-CDS-C-tenellus_contig10375:5204..5368Choristocarpus tenellus KU2346CDSC-tenellus_contig10375 5205..5368 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_C-tenellus_contig10375.2.2prot_C-tenellus_contig10375.2.2Choristocarpus tenellus KU2346polypeptideC-tenellus_contig10375 3742..5368 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_C-tenellus_contig10375.2.2

>prot_C-tenellus_contig10375.2.2 ID=prot_C-tenellus_contig10375.2.2|Name=mRNA_C-tenellus_contig10375.2.2|organism=Choristocarpus tenellus KU2346|type=polypeptide|length=159bp
MYVGLSQIPRTADFILKGGFRRVALQFPDALLPEAPEVLWALQGMLRKAI
GEGMRLGGGDVGDAGVEGLEGLPLIFVTGDTSYGSCCVDEVSAQHLKADA
IVHYAHLRSKTSYTPTGNKTKHSTTMSTYQGQVVLASVPQQLKYLCCMCL
AKETWMSQI
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mRNA from alignment at C-tenellus_contig10375:2731..5368+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_C-tenellus_contig10375.2.2 ID=mRNA_C-tenellus_contig10375.2.2|Name=mRNA_C-tenellus_contig10375.2.2|organism=Choristocarpus tenellus KU2346|type=mRNA|length=2638bp|location=Sequence derived from alignment at C-tenellus_contig10375:2731..5368+ (Choristocarpus tenellus KU2346)
TCCCAGACTATCGGTGAGGACGACGATGCAAAATTGGGTGGGCACGACAG
GTCTATTGCCTTCTCCATACAAGACTTCTACGAGGTTAGTGAGAATTAGT
GTTTCTCACTTTTTATTGTGATGACTCAAGCATGCATGTTGCCAACAACC
TGGTGTTAAGGGGCGTATTTGTTGCGGTGGAAGGAGATAGTATCTTCCAG
TAGGATGCATTGGGACGATATCACAAAAGACGCCCATGTTTGGAGTCATC
CATGCTGATTTGCGATTATTGAAGAACTTCCTGACGTTTCAATTGACATT
CTATCATATAAGTTTCCCCTCAACACGCTGTGACCGTGCAATACGACAAC
CTTTGTTGCATTACTGTTAAGGCTGTTTCCTCGATTGGAGCTGGGTACAA
GAATATCTGTGTTAGTTCGCTCATGTTTTTAATAACTCCAATAGAGTCGT
AAGTAACAGAGTTGTGAATGGGAAAGAAATGTAACTTAACGCTAAGTCTG
CGGACTGCAATGTGCGCATCTATTTCCTGTCCATAGGATACGTTGACAGG
TTGCACAATCTGGTAGAGAAGAGGTGTGGAAGTCGTTGTAGGTACTTTGC
ATAATATGTGTGTTTTACTTCGTTGGTCCTTCGAATGCGATCTATATATA
TGGTGCTCTTGATGTCATGTGGCCGTTGAATAGGCATATTATGCCTAATA
CCTGAAACATGAATGACTGTTCCAGTCCTGAAAGCATGTGTGCGTACACA
AGCCATATATGGAGGAAGATAATCCGACCCTTTATAGAACCAATGTACAG
GAGGTTGTATTGGACAGGGTAGAGACCATGTTATCTTTTCCATAACTGAA
CATTGTTTTCAACATTATCTTCACTGCATCGATCTCTCGTCCAGAAAACT
TATTGGAGTCATGTAGATTTAAAGTAGGGGGGTTCCCATGTTTGCAACAC
TCTTTCTGAAACTCTCTCTCCATCCTCTTGCCTACTCCTTCTACTCCTAC
AACGTAAACAAATGTATGTTGGTTTGAGTCAGATTCCCAGAACTGCAGAT
TTTATCTTGAAGGGGGGATTTCGAAGGGTAGCTCTACAGTTCCCAGATGC
ACTGCTCCCGGAGGCCCCAGAAGTGCTCTGGGCACTACAGGGCATGCTGC
GCAAAGCAATAGGAGAAGGGATGAGATTGGGGGGAGGGGATGTAGGAGAT
GCAGGTGTGGAGGGATTGGAAGGGTTGCCGTTAATATTTGTGACTGGAGA
CACTTCTTATGGCTCCTGCTGTGTGGATGAAGTGTCTGCTCAGCACCTAA
AGGCGGATGCCATTGTGCACTATGGTGAGTGCAGTGCCATACAACTCTAG
ATCTCCCCAGACACACTAAAATACTTACTTGTGTTTAAGTGTGGGTGATA
TTGCACCTTCTACCATCTCACCTCTAATCCATTGTATGCATGTTCATGCA
TCGACATGTATATAGATACAAATGCCCATACGCATATCTCATATTTTCTG
GTGCAGAAGAGGATGCAAAAAAAAAAAATGAACTGCATGATGTGGAGCCC
TATGGCCATGGGTCGACAATCCCCAACTGGATGAATGCCCTCACATAAGA
GGGGCATTCACCTCCTGATTGAACTGTTCCTGTTGTTGGCAACTTGAGGT
AAGGCTTTTTCCTTGACCTCATGGTTACGGTTGTTGTTGGTTTGGACGTG
TGTGTTTTGCCCTGCCTTTGTCTCTGTTGGAACTCTTACATAAACCCCTG
TGCTACCGAGGAGACAGTCAGCTGACTGCTTTCACAGTACCACAGGGGTT
TACTAAAAGTTCTAACAAATGATGCTTTCGTGGGATTGAATATCACTACG
GTTACACATCTACCAAAAGGCAACACACACCACCAGGATACACAACCTCA
CTTTTGTAACTGCCCCGCCGTAGCTAGTCACTGATTAGGGGGATAGCAAC
CCTTGGGCAAAACCTCGACAAGTCTAAGAGCTTGCAATCCCCGGGATTAC
TTTTTGTTGGAACTGTCAGTATACCCCTGTGGTACTGTGAAAGCAGTCAG
CTGACTATCTCCTCAGTAACACAAGGGTTCACTAAGAGTTCCAACAGGCT
CACCTTCTCAGCAATCACCTCCCCCACATTCCTTCCATGGTGTAGATTCT
GTGGAATATGCTTCCACATTATATTAACATACACTCTTTTGTCCATGTGG
AACAGTTGGATTTTAGTTCTACACATCCTCAAAGCAGTTTTTTTTAGTCA
TCTGCCCTCATGTGCAGTACATATTACTTCTCCAGCTGTTTTCTACAATT
CAGTGTGTGTTGTTGTGCTCAAAGGACAGAGTCCATATTCAGCTATAGAC
TCAACCTTGAACGCAACCACTGTCTGGACATGTAGTCCTTCAGAGTCTTC
ACAACACAGTCCAACATCTCCCTCCACCCCCCTTCATTGCACTCTTGACA
AACACATATACACATACCGTCCAGCCCATCTCCGCTCAAAAACAAGCTAT
ACACCAACAGGGAATAAAACGAAACACTCCACAACAATGTCTACGTATCA
AGGACAGGTCGTGCTTGCCTCAGTCCCACAGCAGCTGAAGTACCTGTGTT
GTATGTGTTTGGCAAAGGAGACCTGGATGTCCCAGATC
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Coding sequence (CDS) from alignment at C-tenellus_contig10375:2731..5368+

>mRNA_C-tenellus_contig10375.2.2 ID=mRNA_C-tenellus_contig10375.2.2|Name=mRNA_C-tenellus_contig10375.2.2|organism=Choristocarpus tenellus KU2346|type=CDS|length=477bp|location=Sequence derived from alignment at C-tenellus_contig10375:2731..5368+ (Choristocarpus tenellus KU2346)
ATGTATGTTGGTTTGAGTCAGATTCCCAGAACTGCAGATTTTATCTTGAA
GGGGGGATTTCGAAGGGTAGCTCTACAGTTCCCAGATGCACTGCTCCCGG
AGGCCCCAGAAGTGCTCTGGGCACTACAGGGCATGCTGCGCAAAGCAATA
GGAGAAGGGATGAGATTGGGGGGAGGGGATGTAGGAGATGCAGGTGTGGA
GGGATTGGAAGGGTTGCCGTTAATATTTGTGACTGGAGACACTTCTTATG
GCTCCTGCTGTGTGGATGAAGTGTCTGCTCAGCACCTAAAGGCGGATGCC
ATTGTGCACTATGCCCATCTCCGCTCAAAAACAAGCTATACACCAACAGG
GAATAAAACGAAACACTCCACAACAATGTCTACGTATCAAGGACAGGTCG
TGCTTGCCTCAGTCCCACAGCAGCTGAAGTACCTGTGTTGTATGTGTTTG
GCAAAGGAGACCTGGATGTCCCAGATC
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