mRNA_A-nodosum_M_contig10195.1.1 (mRNA) Ascophyllum nodosum dioecious

BLAST of mRNA_A-nodosum_M_contig10195.1.1 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 783 bits (2023), Expect = 6.350e-273
Identity = 407/640 (63.59%), Postives = 492/640 (76.88%), Query Frame = 1

Query:    1 MDLVSFFAGRSEVLRVAPRHRIGLLNAAARANIQSATVTSSPLTDAGLDGTGEVIQVIDTGLDETSCYFVDEDGEEIAHGHYFEEL--GFTSGSDV--YAPFTGGDFSYDLNRRKIIQYINLVKSDSEPGQYGDY--FTTSSGRRFAWLSAADFEQDEATGHGTHTAGSAAGATLNAPAETVTCSGSDDLGCVGGCINPVSPTDDLLSFS-QFLYFDLDRLCPSFDCGGYGEEVCLGDDVDKTLTEHGGIAQGAKLAIFDIFYDDFGLGSYAGNGLWEACLEAGCKIHSNSYGGDNVCEIGAADVEYDDFMYNHPENLLIFAAGNDGDLDDGREVCTIGSPAIAKNVLAIGSTSSGKTRLTLTGSDGEARTGSNGVADIDTVSYFSSYGPTQDNRIKPELVAPGDLIYSAASDGTDSHSCRLWAHHGTSMSCPIVAGAAAMVRQYFMDDNFYATDVITRGLCDDGFVCESFSPSSATVKALLINSANLMGGSSEPNFSRGFGRVHMEAGMPLGGDGDLVVFVADAANTAISARTTQEYLFHVDGNAGLDLRATISWIDPASTTFSAKQLVHDLDLTVTSPIGTTHTMWSTGVTDVYNVNERVIIDAADVESGTWTISVWANELGTDVQRYSLVVNGAISS 1899
            M LVSFFAGR EVLRV+PRH   +LN AAR  IQSA+ T +PLTDAGLDGTGEVIQV+DTGLDETSC+F+D+ GEE+ HG++FEEL  G T  S +  Y  F  GDF+Y   RRK+IQYI LV  DS      DY  F+T+ G  + +L   +F QD++ GHGTHTAGSAAGATLN PAET+TCSG + LGCVGGCI+    TDDL+S++ Q    DLDRLCP + C G   + CL D+VD+TLT++GG+AQGAKL+IFD+F++ FGL    GNG+WE CLEAGCK+HS S GGD +C  G +++ YDDFMY +PENLLIFAAGNDG+ +  R VCT+ SPAI KNVLA+G+++SG TR+  T           G A ID V+ FSSYG T D RIKPE+VAPGD++YSAASDGTDSHSCRLWA+ GTSMSCPIVAGAAAMVRQYF+D+ FY  DV  RG C DGF+CE FSPS+ATVKALLINSANLMG SS+P+  RGFGR+H+E GMPL G+GDL +FVADA +T+I+  T QEYLF VD +AGL+LRAT+SWID  +T+ SA QLV+DLDL V SP GT + MW + VTD  NVNERVI+DA DVE+GTWT+ V++N L TD Q YSLVVNGAIS+
Sbjct:  321 MGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASATDTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDGGEEVDHGYFFEELFVGSTGSSQLIYYPVFEAGDFTYTEERRKVIQYIELVNPDS------DYVAFSTTQGGVYPFLPPDEFGQDDSAGHGTHTAGSAAGATLNDPAETLTCSGDETLGCVGGCIDESETTDDLVSYAIQIALPDLDRLCPMYGCDG-STDPCLSDEVDETLTKNGGMAQGAKLSIFDVFFEVFGLIEVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDGETE--RSVCTMASPAIGKNVLAVGASTSGDTRVIST----------EGGAGIDMVADFSSYGFTTDGRIKPEVVAPGDMVYSAASDGTDSHSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDETFYTADVTARGFCSDGFICEGFSPSAATVKALLINSANLMGESSQPDAYRGFGRIHLEQGMPLEGEGDLALFVADALDTSIAEDTVQEYLFDVDADAGLELRATLSWIDYPATSTSAMQLVNDLDLVVVSPSGTVYRMWGSDVTDNRNVNERVIVDADDVETGTWTVLVYSNSLVTDSQSYSLVVNGAISA 941          

BLAST of mRNA_A-nodosum_M_contig10195.1.1 vs. uniprot
Match: D8LNG4_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG4_ECTSI)

HSP 1 Score: 773 bits (1996), Expect = 2.890e-272
Identity = 407/670 (60.75%), Postives = 490/670 (73.13%), Query Frame = 1

Query:    1 MDLVSFFAGRSEVLRVAPRHRIGLLNAAARANIQSATVTSSPLTDAGLDGTGEVIQVIDTGLDETSCYFV-DEDGEEIAHGHYFEELGF----TSGS----------------------------DVYAPFTGGDFSYDLNRRKIIQYINLVKSDSEPGQYGDYFTTSSGRRFAWLSAADFEQDEATGHGTHTAGSAAGATLNAPAETVTCSGSDDLGCVGGCINPVSPTD-DLLSFSQFLYFDLDRLCPSF---DCGGYGEEVCLGDDVDKTLTEHGGIAQGAKLAIFDIFYDDFGLGSYAGNGLWEACLEAGCKIHSNSYGGDNVCEIGAADVEYDDFMYNHPENLLIFAAGNDGDLDDGREVCTIGSPAIAKNVLAIGSTSSGKTRLTLTGSDGEARTGSNGVADIDTVSYFSSYGPTQDNRIKPELVAPGDLIYSAASDGT-DSHSCRLWAHHGTSMSCPIVAGAAAMVRQYFMDDNFYATDVITRGLCDDGFVCESFSPSSATVKALLINSANLMGGSSEPNFSRGFGRVHMEAGMPLGGDGDLVVFVADAANTAISARTTQEYLFHVDGNAGLDLRATISWIDPASTTFSAKQLVHDLDLTVTSPIGTTHTMWSTGVTDVYNVNERVIIDAADVESGTWTISVWANELGTDVQRYSLVVNGAIS 1896
            M LVSF A + EVLRVAPR    LLNAAARANIQSATVT +PLTDAGLDGTG+V+QVID+G+DE+SC+F  DE G+ + HGHYFEE G     TS S                             +   F GGDF+   +RRKIIQYINL+KSD   G +GD FTTS G+++ WLSA  FE D+  GHGTH AGS AGATLN PAE ++C+ ++ + CVG C++     D D L+       DLDRLCP F   DC G   + CL DDV +TLT +GG+AQGAKLAIFD FYD FGL  + G GLWE C +A CKIHSNSYGGD  C +G+ DV YD++M ++PENLLIFAAGNDG+L   R  CTIGSPAIAKNVLA+G+++SG TRL+ T   G           IDTV+ FSSYGPT D+RIKPE++APGD IYSAASDG+ D+HSCRLWA+ GTSMS PIVAGA+AM+RQYFM++ F+ATD+  RG CD+   CE+FSPSSAT+KAL+INSANLMG SSEPN SRGFGR+H+EAGMPLGG+G L +FV+D+  T+I   T QEY F V+  AGLDLR T+SW+DP +TTFSA QLVHDLDL V SP GT +TMW++GVTD  NVNERVI+DA DVE+GTW++ +WA+ L TDVQ YSLVVNGAIS
Sbjct:    1 MGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPSLTSSSFSSPXXXXXXXXXXXPWATADHHTGSYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLSADHFEYDDEAGHGTHVAGSVAGATLNTPAELISCNDTETMSCVGACVDDDEANDVDDLATPYVEIADLDRLCPLFSDYDCDGLETDKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDTFGLSPFVGTGLWEPCADADCKIHSNSYGGDFECSLGSNDVLYDEWMVSNPENLLIFAAGNDGELR--RSTCTIGSPAIAKNVLAVGASTSGDTRLSATPGSG-----------IDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSVDTHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSATLKALMINSANLMGESSEPNGSRGFGRIHLEAGMPLGGEGSLALFVSDSNYTSIPELTRQEYNFDVNATAGLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSSNVNERVIVDADDVETGTWSVWIWADNLTTDVQSYSLVVNGAIS 657          

BLAST of mRNA_A-nodosum_M_contig10195.1.1 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 762 bits (1968), Expect = 1.450e-263
Identity = 404/669 (60.39%), Postives = 484/669 (72.35%), Query Frame = 1

Query:    1 MDLVSFFAGRSEVLRVAPRHRIGLLNAAARANIQSATVTSSPLTDAGLDGTGEVIQVIDTGLDETSCYFV-DEDGEEIAHGHYFEELGF------------TSGSD-------------------VYAPFTGGDFSYDLNRRKIIQYINLVKSDSEPGQYGDYFTTSSGRRFAWLSAADFEQDEATGHGTHTAGSAAGATLNAPAETVTCSGSDDLGCVGGCINPVSPTD-DLLSFSQFLYFDLDRLCPSF---DCGGYGEEVCLGDDVDKTLTEHGGIAQGAKLAIFDIFYDDFGLGSYAGNGLWEACLEAGCKIHSNSYGGDNVCEIGAADVEYDDFMYNHPENLLIFAAGNDGDLDDGREVCTIGSPAIAKNVLAIGSTSSGKTRLTLTGSDGEARTGSNGVADIDTVSYFSSYGPTQDNRIKPELVAPGDLIYSAASDGT-DSHSCRLWAHHGTSMSCPIVAGAAAMVRQYFMDDNFYATDVITRGLCDDGFVCESFSPSSATVKALLINSANLMGGSSEPNFSRGFGRVHMEAGMPLGGDGDLVVFVADAANTAISARTTQEYLFHVDGNAGLDLRATISWIDPASTTFSAKQLVHDLDLTVTSPIGTTHTMWSTGVTDVYNVNERVIIDAADVESGTWTISVWANELGTDVQRYSLVVNGAIS 1896
            M LVSF A + EVLRVAPR    LLNA+ARANIQSATVT +PLTDAGLDGTG+V+QVID+G+DE+SC+F  DE G+ + HGHYFEE G             TS  D                   +   F GGDF+   +RRKIIQYINL+KSD   G +GD FTTS G+++ WL A  FE D   GHGTH AGS AGATLN PAE ++C  ++ + CVG C++     D D L+       DLDRLCP F   DC     E CL DDV +TLT +GG+AQGAKLAIFD FYD+FGL  + GNGLWE C EA CKIHSNSYGGD  C +G  DV YD +M ++PENLLIFAAGNDG  +  R  CT+ SPAIAKNVLA+G+++SG TRL+ T   G           IDTV+ FSSYGPT D+RIKPE++APGD IYSAASDG+ DSHSCRLWA+ GTSMS PIVAGA+AM+RQYFM++ F+ATD+  RG CD+   CE+FSPSSAT+KAL+INSANLMGGSSEP+ SRGFGR+H+EAGMPL G+G L +FV+D+  T+I  RT QEY F V+  A LDLR T+SW+DP +TTFSA QLVHDLDL V SP GT +TMW++GVTD  NVNERVI+DA DVE+GTW++ +WA+ L TDVQ YSLVVNGAIS
Sbjct:  312 MGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSSFSSPTSDDDDSLGWPRATDDHRTGSYYQIAEEFDGGDFTIFPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLPADRFEYDYEAGHGTHVAGSVAGATLNTPAELISCKDTETMSCVGACVDDDEANDVDDLATPNVEIVDLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDNFGLSPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDG--ERRRSTCTMSSPAIAKNVLAVGASTSGDTRLSATPGLG-----------IDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYAGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSATLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLRGEGSLALFVSDSNYTSIPERTRQEYNFDVNATARLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSANVNERVIVDAGDVETGTWSVWIWADNLTTDVQSYSLVVNGAIS 967          

BLAST of mRNA_A-nodosum_M_contig10195.1.1 vs. uniprot
Match: A0A6H5K365_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K365_9PHAE)

HSP 1 Score: 718 bits (1853), Expect = 4.990e-251
Identity = 388/648 (59.88%), Postives = 460/648 (70.99%), Query Frame = 1

Query:    1 MDLVSFFAGRSEVLRVAPRHRIGLLNAAARANIQSATVTSSPLTDAGLDGTGEVIQVIDTGLDETSCYFVDEDGEEIAHGHYFEELG-------FTSGSDVYAPFTGGDFSYDLNRRKIIQYINLVKSDSEPGQYGDYFTTSSGRRFAWLSAADF-EQDEAT-------GHGTHTAGSAAGATLNAPAETVTCSGSDDLGCVGGCINPVSPTDDLLSFS-QFLYFDLDRLCPSFDCGGYGEEVCLGDDVDKTLTEHGGIAQGAKLAIFDIFYDDFGLGSYAGNGLWEACLEAGCKIHSNSYGGDNVCEIGAADVEYDDFMYNHPENLLIFAAGNDGDLDDGREVCTIGSPAIAKNVLAIGSTSSGKTRLTLTGSDGEARTGSNGVADIDTVSYFSSYGPTQDNRIKPELVAPGDLIYSAASDGTDSHSCRLWAHHGTSMSCPIVAGAAAMVRQYFMDDNFYATDVITRGLCDDGFVCESFSPSSATVKALLINSANLMGGSSEPNFSRGFGRVHMEAGMPLGGDGDLVVFVADAANTAISARTTQEYLFHVDGNAGLDLRATISWIDPASTTFSAKQLVHDLDLTVTSPIGTTHTMWSTGVTDVYNVNERVIIDAADVESGTWTISVWANELGTDVQRYSLVVNGAIS 1896
            M LVS  AGR EVLRV+PR    +LNAAAR  +QSA+ T +PLTDAGLDGTGE+IQVID+GLDETSCYF+D+ GEE+ HG+YF+EL          S  +    F GGDFSY   RRKIIQYI+LV  DS    Y  ++T+       +L    F E DEA                SAAGATLN PAET+TC+ +D L CVG CI+ +  TDDL+S S Q    DLDRLCP + C    +  CL DDV +TL+ +GG+AQGAKLAIFD+FY D+   S  GN LWE C EAGCK+HSNS G D  C I + D+  D FMY + E+LLIFAAGN+G   + R  CTI +P I KN LAIG+++SG TR+  T           G   I+TV+ FSS+G T D+RIKPE+VAPGD +YSAASDGTD+HSCRLWA+ GTSMSCPIVAGAAAM+RQYFMD+ FY  DV  RG C D F+CE FSPS+ATVKALLINSANLMGGSSEP+  RGFGRVH+EAGMPL G+G+LV+FVADA +T+I+  T  EYLF VD +AGLDLRAT+SWIDP ST  SA QLVH+LDL V SP GTT+ MW +  TD  NVNERVI+DA DV +GTWT+ VWAN L TD Q YSLVVNGAIS
Sbjct:   33 MGLVSVLAGRPEVLRVSPRPTKRVLNAAARGIVQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFDELAQAAVYSSLYSSDETATIFNGGDFSYYPERRKIIQYIDLVNPDSP---YSSFYTSQGSYIGYFLPPDQFGEDDEARVALRKKDPSQARVGRSAAGATLNNPAETITCNVTDTLSCVGACIDLLDVTDDLVSISHQTGGTDLDRLCPMYGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGDYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAEHLLIFAAGNEG--GEERSTCTINTPGIGKNSLAIGASTSGDTRVISTV----------GATGINTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFMDETFYMADVTARGFCSDVFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGNLVLFVADALDTSIAEGTLDEYLFDVDADAGLDLRATLSWIDPPSTATSAVQLVHNLDLVVVSPDGTTYRMWGSETTDNRNVNERVIVDATDVVTGTWTVWVWANSLITDSQSYSLVVNGAIS 665          

BLAST of mRNA_A-nodosum_M_contig10195.1.1 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 718 bits (1854), Expect = 9.560e-248
Identity = 383/640 (59.84%), Postives = 450/640 (70.31%), Query Frame = 1

Query:    1 MDLVSFFAGRSEVLRVAPRHRIGLLNAAARANIQSATVTSSPLTDAGLDGTGEVIQVIDTGLDETSCYFVDEDGEEIAHGHYFEELGFT-------SGSDVYAPFTGGDFSYDLNRRKIIQYINLVKSDSEPGQYGDYFTTSSGRRFAWLSAADFEQDEATGHGTHTAGSAAGATLNAPAETVTCSGSDDLGCVGGCINPVSPTDDLLSFS-QFLYFDLDRLCPSFDCGGYGEEVCLGDDVDKTLTEHGGIAQGAKLAIFDIFYDDFGLGSYAGNGLWEACLEAGCKIHSNSYGGDNVCEIGAADVEYDDFMYNHPENLLIFAAGNDGDLDDGREVCTIGSPAIAKNVLAIGSTSSGKTRLTLTGSDGEARTGSNGVADIDTVSYFSSYGPTQDNRIKPELVAPGDLIYSAASDGTDSHSCRLWAHHGTSMSCPIVAGAAAMVRQYFMDDNFYATDVITRGLCDDGFVCESFSPSSATVKALLINSANLMGGSSEPNFSRGFGRVHMEAGMPLGGDGDLVVFVADAANTAISARTTQEYLFHVDGNAGLDLRATISWIDPASTTFSAKQLVHDLDLTVTSPIGTTHTMWSTGVTDVYNVNERVIIDAADVESGTWTISVWANELGTDVQRYSLVVNGAIS 1896
            M LVSF AGR EVLRV+PR    +LNAAAR  IQSA+ T +PLTDAGLDGTGE+IQVID+GLDETSCYF+D+ GEE+ HG+YF+ELG         S  +    F GGDFSY   RRK                                           GHGTHTAGSAAGATLN PAET+TC+ +D L CVG CI+ +  TDDL+S S Q    DLDRLCP + C    +  CL DDV +TL+ +GG+AQGAKLAIFD+FY  +   S  GN LWE C EAGCK+HSNS G D  C I + D+  D FMY + ENLLIFAAGN+G ++  R  CTI +P I KN LAIG+++SG TR+  T           G   IDTV+ FSS+G T D+RIKPE+VAPGD +YSAASDGTD+HSCRLWA+ GTSMSCPIVAGAAAM+RQYF+D+ FY  DV  RG C DGF+CE FSPS+ATVKALLINSANLMGGSSEP+  RGFGRVH+EAGMPL G+GDLV+FVADA +T+I+  T +EYLF VD +AGLDLRAT+SWIDP +T  SA QLVH+LDL V SP GTT+ MW +  TD  NVNERVI+DA DV +GTWT+ VWAN L TD Q YSLVVNGAIS
Sbjct:  319 MGLVSFLAGRPEVLRVSPRPTKRVLNAAARGVIQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFDELGRAAVYSSSYSSDETATIFNGGDFSYYPERRKA------------------------------------------GHGTHTAGSAAGATLNDPAETITCNVTDTLSCVGACIDLLDATDDLVSISSQTDGTDLDRLCPMYGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGGYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAENLLIFAAGNEGGIE--RSTCTINTPGIGKNSLAIGASTSGDTRVISTV----------GATGIDTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFVDETFYTADVTARGFCSDGFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGDLVLFVADALDTSIAGGTLEEYLFDVDADAGLDLRATLSWIDPPATATSAVQLVHNLDLVVVSPDGTTYRMWGSDTTDNRNVNERVIVDATDVVTGTWTVWVWANNLVTDSQSYSLVVNGAIS 904          

BLAST of mRNA_A-nodosum_M_contig10195.1.1 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 665 bits (1715), Expect = 5.170e-228
Identity = 360/617 (58.35%), Postives = 429/617 (69.53%), Query Frame = 1

Query:    1 MDLVSFFAGRSEVLRVAPRHRIGLLNAAARANIQSATVTSSPLTDAGLDGTGEVIQVIDTGLDETSCYFVDEDGEEIAHGHYFEELGFTSGSDVYAPFTGGDFSYDLNRRKIIQYINLVKSDSEPGQYGDYFTTSSGRRFAWLSAADFEQDEATGHGTHTAGSAAGATLNAPAET-VTCSGSDDLGCVGGCINP--VSPTDDLLS----FSQFLYFDLDRLCPSFDCGGYGEEVCLGDDVDKTLTEHGGIAQGAKLAIFDIFYDDFGLGSYAGNGLWEACLEAGCKIHSNSYGGDNVCEIGAADVEYDDFMYNHPENLLIFAAGNDGDLDDGREVCTIGSPAIAKNVLAIGSTSSGKTRLTLTGSDGEARTGSNGVADIDTVSYFSSYGPTQDNRIKPELVAPGDLIYSAASDGTDSHSCRLWAHHGTSMSCPIVAGAAAMVRQYFMDDNFYATDVITRGLCDD-GFVCESFSPSSATVKALLINSANLMGGSSEPNFSRGFGRVHMEAGMPLGGDGDLVVFVADAANTAISARTTQEYLFHVDGNAGLDLRATISWIDPASTTFSAKQLVHDLDLTVTSPIGTTHTMWST-GVTDVYNVNERVIIDAADVESGTWT 1824
            M LVSF + R EVLRV+PRH   LLNA AR  IQSAT+T +PLTDAGLDGTG+VIQVIDTGLDETSC+F+D+DGEE+ HGHY+E LG                              ++ S S                          +D+  GHGTH AGSA GAT+  PAET  TCSG++  GCVGGCI+    S  DDL +    +S +   D+DRLCP  DC    +  CL DDV +TL +HGG+AQGAKLA FD+F D      + GNGLWE CLEAGCKIHS S G D  C +   DV YDDFMYN+PENL++ AAGN GD +DGR VCT+ SPAI KN LA+G+TSSG+TR+T T +DG+   G+NG AD+DTV+ FSSYGPTQD RIKPE+VAPGD IYSA SDG+D+HSCRL A  GTSM+CPIVAGA+AMVRQYFMD++FY  D   RG  DD G  CESFSPSSATVKALLINSANLMGGSSEP+  RGFGR+H+E GMPL G+G L +FVAD++ T++    +Q+++F VD NA LD RAT+SWIDP +++ S  QLVHDLDL V SP G TH MW+T G  D  NVNERVII A DVESGT+T
Sbjct:  317 MGLVSFLSNRLEVLRVSPRHADRLLNAVARTIIQSATITEAPLTDAGLDGTGQVIQVIDTGLDETSCFFIDDDGEEVPHGHYYEALG-----------------------------EIIPSSSSA------------------------RDDVEGHGTHCAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLFTLMPEYSPY-DIDIDRLCPMIDCDDATDPRCLSDDVGQTLADHGGMAQGAKLAFFDVFADGSSGTHFVGNGLWEPCLEAGCKIHSMSIGADFECSVCEKDVLYDDFMYNNPENLVVVAAGNAGDTEDGRTVCTMYSPAIGKNALAVGATSSGETRITKTSADGDLIDGTNGYADVDTVAQFSSYGPTQDGRIKPEVVAPGDAIYSARSDGSDNHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYVADQAARGWSDDSGRRCESFSPSSATVKALLINSANLMGGSSEPDGYRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELCSQDFIFDVDANANLDFRATLSWIDPPASSASGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAEDVESGTYT 879          

BLAST of mRNA_A-nodosum_M_contig10195.1.1 vs. uniprot
Match: A0A6H5JKA8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKA8_9PHAE)

HSP 1 Score: 672 bits (1733), Expect = 2.760e-227
Identity = 370/702 (52.71%), Postives = 470/702 (66.95%), Query Frame = 1

Query:    1 MDLVSFFAGRSEVLRVAPRHRIGLLNAAARANIQSATVTSSPLTDAGLDGTGEVIQVIDTGLDETSCYFVDEDGEE-IAHGHYFEELGFTSGS------------DVYAPFTGGDFSYDLNRRKIIQYINLVKSDSEPG--------------------------QYGDYFTTSSGRRFAWLSAADFEQDEATGHGTHTAGSAAGATLNAPAETVT------CSGSDDLGCVGGCINPVSP--TDDLLSFSQFLYF-------DLDRLCPSFDCGGYGEEVCLGDDVDKTLTEHGGIAQGAKLAIFDIFYDDFGLG-SYAGNGLWEACLEAGCKIHSNSYGGDNVCEIGAADVEYDDFMYNHPENLLIFAAGNDGDLDDGREVCTIGSPAIAKNVLAIGSTSSGKTRLTLTGSDGEARTGSNGVADIDTVSYFSSYGPTQDNRIKPELVAPGDLIYSAASDGTDSHSCRLWAHHGTSMSCPIVAGAAAMVRQYFMDDNFYATDVITRGLCDDGFVCESFSPSSATVKALLINSANLMGGSSEPNFSRGFGRVHMEAGMPLGGDGDLVVFVADAANTAISARTTQEYLFHVDGNAGLDLRATISWIDPASTTFSAKQLVHDLDLTVTSPIGTTHTMWSTGVTDVYNVNERVIIDAADV---------------ESGTWTISVWANEL-GTDVQRYSLVVNGAI 1893
            M L+SF A R EVLRV+P   I LLNAAA  N+QSA++T SP+TDAGLDGTGEVIQ++DTGLD  SC+FVDEDG E +AHG+YFEE+G  + S             +   FTGGDF++DL+RRK +       S   P                            YG+ + TS+G ++ W+SAA+F +D+A GHGTHTAGSAAGA +++P+ + T      C    +L CVGGCI+   P   DDL++   + YF       DLDRLCP  DC    EE CLGDD  +TL +HGG+A+GA+LAIFDI  D  G+G   AGNG+WE  +EAG K+ SNS+G    C+  + DV +DDFMY + + LL+FAAGNDGD   G   CT+G+PA+AKNVLA+G+++SG  RL+ T  DG+   G + V DID ++YFSS+GPT DNRIKPE+VAPGD+IYSAA  G  +++CRLW + GTSMS PIVAGAAA+VRQYF ++ FYA DV  RG CD  F CE F+PS+ATVKA+LINSANLMGGS+EP+  RGFGRVH+EAGMPL G+GD  +FVAD+A T+ +  T  EY F VD  AG+DLRAT+SWIDP +TTFS+ QLVHDLDL+V SP G  ++MW  G  D  N NERVI+ A+++                 GTWT+ VW+  L G + Q YSLVV GAI
Sbjct:  380 MALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSASLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGYYFEEIGVEASSFGSWFTSWDFNVQIARVFTGGDFNFDLSRRKGLVVDAAHPSCDSPNISRRFALGDVRAWSGENPACTANNSTNYGESWATSAGEQYPWISAAEFAEDDAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDPFGEDDLITTWDYQYFWAGYEAIDLDRLCPRLDCDDLPEEYCLGDDEAETLQQHGGVARGAQLAIFDIINDYTGVGIDMAGNGMWEPAMEAGSKLSSNSWGIYAYCDYLSLDVLFDDFMYRNQDQLLLFAAGNDGDDWSG---CTMGTPALAKNVLAVGASTSGPGRLSFTDEDGDPTDGDSRVQDIDAMAYFSSFGPTLDNRIKPEVVAPGDMIYSAAGSGDGNYTCRLWQYAGTSMSTPIVAGAAALVRQYFKNETFYAHDVEERGFCDS-FGCEPFAPSAATVKAMLINSANLMGGSAEPDGLRGFGRVHLEAGMPLAGEGDNALFVADSAVTSTAELTLTEYSFAVDAAAGIDLRATLSWIDPPATTFSSVQLVHDLDLSVYSPTGLRYSMWRDGTVDSLNNNERVIVPASNILDQTSSDATTAEDGESDGTWTVRVWSKRLAGNETQSYSLVVTGAI 1077          

BLAST of mRNA_A-nodosum_M_contig10195.1.1 vs. uniprot
Match: A0A2I6U1S5_SACJA (Subtilisin n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1S5_SACJA)

HSP 1 Score: 650 bits (1676), Expect = 2.650e-221
Identity = 356/639 (55.71%), Postives = 433/639 (67.76%), Query Frame = 1

Query:    1 MDLVSFFAGRSEVLRVAPRHRIGLLNAAARANIQSATVTSSPLTDAGLDGTGEVIQVIDTGLDETSCYFVDEDGEEIAHGHYFEELGFTSGSDVYAPFTGGDFSYDLNRRKIIQYINLVKSDSEPGQYGDYFTTSSGRRFAWLSAADFEQDEATGHGTHTAGSAAGATLNAPAET-VTCSGSDDLGCVGGCINP--VSPTDDLLSF---SQFLYFDLDRLCPSFDCGGYGEEVCLGDDVDKTLTEHGGIAQGAKLAIFDIFYDDFGLGSYAGNGLWEACLEAGCKIHSNSYGGDNVCEIGAADVEYDDFMYNHPENLLIFAAGNDGDLDDGREVCTIGSPAIAKNVLAIGSTSSGKTRLTLTGSDGEARTGSNGVADIDTVSYFSSYGPTQDNRIKPELVAPGDLIYSAASDGTDSHSCRLWAHHGTSMSCPIVAGAAAMVRQYFMDDNFYATDVITRGLCDD-GFVCESFSPSSATVKALLINSANLMGGSSEPNFSRGFGRVHMEAGMPLGGDGDLVVFVADAANTAISARTTQEYLFHVDGNAGLDLRATISWIDPASTTFSAKQLVHDLDLTVTSPIGTTHTMWST-GVTDVYNVNERVIIDAADVESGTWTISVWANELGTDVQRYSLVVNGAI 1893
            M LVSF + R EV RV+PRH   LLNA AR  IQSAT+T +PLT AGLDGTG+VIQVI                                                       QYINL+K+DS  G      TTS G R  W+S   F+ D+   HGTHTAGSA GAT+  PAET  TCSG++  GCVGGCI+    S  DDLL+    +     D+DR+CP   C    E  CL D V++TL +HGG+AQGAKL+ FDI+  +     + GNGLWE CLEAGCKIHS S+G D  C +   D+ YDDFMYN+PENL++ AAGN G+++DGR VCT+ SPAI KN LA+G+TSSG+TR+ +T +DG+   G+NG +D+DTV+ FSSYGPTQD RIKPE+VAPGD +YSA SDG+D HSCRL A  GTSM+CPIVAGA+AMVRQYFMD++FY  D   RG CDD G  CESFSPSSATVKALLINSANLMGGSSEP+  RGFGR+H+E GMPL G+G L +FVAD++ T++   ++Q+++F VD NA LD RAT+SWIDP +++ S  QLVHDLDL V SP G TH MW+T G  D  NVNERVII A +VESGT+T+ V  N L TD Q YSLVVNGA+
Sbjct:  317 MGLVSFLSSRLEVSRVSPRHADRLLNAVARTIIQSATITKAPLTAAGLDGTGQVIQVI-------------------------------------------------------QYINLIKADSVSGPASS--TTSGGNRVWWVSPDGFQGDDVDDHGTHTAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLLTLMPENSSYVADIDRICPMLGCDEENEPWCLSDGVEQTLADHGGMAQGAKLSFFDIYDGNMLFEQFIGNGLWEPCLEAGCKIHSLSFGADLECAVRDTDLLYDDFMYNNPENLVVVAAGNAGEIEDGRTVCTMYSPAIGKNALAVGATSSGETRVGVTSADGDRADGTNGYSDVDTVAVFSSYGPTQDGRIKPEVVAPGDAVYSARSDGSDVHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYLADQAARGWCDDSGRQCESFSPSSATVKALLINSANLMGGSSEPDGFRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELSSQDFVFDVDANANLDFRATLSWIDPPASSTSGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAENVESGTYTVRVSTNHLLTDSQNYSLVVNGAM 898          

BLAST of mRNA_A-nodosum_M_contig10195.1.1 vs. uniprot
Match: A0A2I6U1Q9_SACJA (Subtilisin n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1Q9_SACJA)

HSP 1 Score: 629 bits (1623), Expect = 3.020e-217
Identity = 353/634 (55.68%), Postives = 412/634 (64.98%), Query Frame = 1

Query:    1 MDLVSFFAGRSEVLRVAPRHRIGLLNAAARANIQSATVTSSPLTDAGLDGTGEVIQVIDTGLDETSCYFVDEDGEEIAHGHYFEELGFTSGSDVYAPFTGGDFSYDLNRRKIIQYINLVKSDSEPGQYGDYFTTSSGRRFAWLSAADFEQDEATGHGTHTAGSAAGATLNAPAET-VTCSGSDDLGCVGGCINPVSPTDDLLSFSQFLYFDLDRLCPSFDCGGYGEEVCLGDDVDKTLTEHGGIAQGAKLAIFDIFYDDFGLGSYAGNGLWEACLEAGCKIHSNSYGGDNVCEIGAADVEYDDFMYNHPENLLIFAAGNDGDLDDGREVCTIGSPAIAKNVLAIGSTSSGKTRLTLTGSDGEARTGSNGVADIDTVSYFSSYGPTQDNRIKPELVAPGDLIYSAASDGTDSHSCRLWAHHGTSMSCPIVAGAAAMVRQYFMDDNFYATDVITRGLCDD-GFVCESFSPSSATVKALLINSANLMGGSSEPNFSRGFGRVHMEAGMPLGGDGDLVVFVADAANTAISARTTQEYLFHVDGNAGLDLRATISWIDPASTTFSAKQLVHDLDLTVTSPIGTTHTMWST-GVTDVYNVNERVIIDAADVESGTWTISVWANELGTDVQRYSLVVNGAI 1893
            M LVSF + R EVLRV+PRH   LLNA AR  IQSAT+T +PLTDAGLDGTG+VIQVI                                                       QYINL+K+DS PG      TTS G +  W S   F+ D+  GHGTH AGSA GAT+  PAET  TCSG++  GCVGGCI+          +SQ +  +LDR CP   C    +  CL DDV +TL  HGG+AQGAKLA FDIF D   L  Y GNGLWE CLEAGCKIHS S GGD  C +   DV YDDFMYN+PENL++ AAGN GD+ DGR VCT+ SPAI                   T +DG+   G+NG AD+DTV+ FSSYGPTQD RIKPE+VAPGD  YSA SDG D HSCRL A  GTSM+CPIVAGA+AMVRQYFMD++FY  D   RG CDD G  CESFSPSSATVKALLINSANLMGGSSEP+  RGFGR+H+E GMPL G+G L +FVAD++ T++   ++ +++F VD +A LD RAT+SWIDP +++ S  QLVHDLDL V SP G TH MW+T G  D  NVNERVII AADVESGT+T+ V  N L TD Q YSLVVNGA+
Sbjct:  109 MGLVSFLSSRLEVLRVSPRHAGRLLNAVARTIIQSATITEAPLTDAGLDGTGQVIQVI-------------------------------------------------------QYINLIKADSVPGPVSS--TTSGGDKVRWFSPDHFQGDDIEGHGTHCAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDA--------EYSQ-IGVNLDRFCPMIQCDDATDPRCLSDDVGQTLANHGGMAQGAKLAFFDIFADGGALTPYVGNGLWEPCLEAGCKIHSMSIGGDLECAVHGLDVLYDDFMYNNPENLVVVAAGNAGDIQDGRTVCTMYSPAIGT-----------------TSADGDLADGTNGYADVDTVAQFSSYGPTQDGRIKPEVVAPGDTTYSARSDGGDDHSCRLSAQFGTSMACPIVAGASAMVRQYFMDESFYLADQAARGWCDDSGRQCESFSPSSATVKALLINSANLMGGSSEPDGFRGFGRIHLEMGMPLDGNGSLSLFVADSSYTSLPELSSHDFVFDVDADANLDFRATLSWIDPPASSTSGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAADVESGTYTVRVSTNHLLTDSQGYSLVVNGAM 659          

BLAST of mRNA_A-nodosum_M_contig10195.1.1 vs. uniprot
Match: A0A6H5L2G3_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L2G3_9PHAE)

HSP 1 Score: 624 bits (1608), Expect = 1.770e-214
Identity = 343/647 (53.01%), Postives = 444/647 (68.62%), Query Frame = 1

Query:    1 MDLVSFFAGRSEVLRVAPRHRIGLLNAAARANIQSATVTSSPLTDAGLDGTGEVIQVIDTGLDETSCYFVDEDGEEIAHGHYFEELG-----FTSGSD----VYAPFTGGDFSYDLNRRKIIQYINLVKSDSEPGQYGDYFTTSSGRRFAWLSAADFEQDEATGHGTHTAGSAAGATLNAPAETVTCSGSDD-LGCVGGCINPVSPTDDLLSFSQFLYFDLDRLCPSFDCGGYGEEVCLGDDVDKTLTEHGGIAQGAKLAIFDIFYDDFGLGSYAGNGLWEACLEAGCKIHSNSYGGDNVCEIGAADVEYDDFMYNHPENLLIFAAGNDGDLDDGREVCTIGSPAIAKNVLAIGSTSSGKTRLTLTGSDGEARTGSNGVADIDTVSYFSSYGPTQDNRIKPELVAPGDLIYSAASDGTDSHSCRLWAHHGTSMSCPIVAGAAAMVRQYFMDDNFYATDVITRGLCDDGFVCESFSPSSATVKALLINSANLMGGSSEPNFSRGFGRVHMEAGMPLGGDGDLVVFVADAANTAISARTTQEYLFHVDGNAGLDLRATISWIDPASTTFSAKQLVHDLDLTVTSPIGTTHTMWSTGVTDVYNVNERVIIDAADVE----SGTWTISVWANELGT-DVQRYSLVVNGAIS 1896
            M LVSFFA R EVL+V   +R   LNAAARA IQSA+V S+PLTDAGLDG+GEVIQ+ID G++ET+C+    DG E+ HG+YF+ +      F S S+      A FTGGDF YD +RRK+IQYI+L+  D+  G       +SSG  F  +SA  F  D+  GHGTHTA + AG+T+++PAE V+C+ + + +GC GGCI   S       + +    D++RLCP+F C G   + CL DD   TL+EHGG+AQGAK+A  DIFY D+  G  AGNGLWE C +AGCKIHSNSYG D  C++ A DV YD++MY + E+LL+F+AGN G   D R+ CTI SP IAKNVL +GSTSSG TRL+ T  DG      + ++ ID VS FS YG T+D RIKPE+VAPGD+IYSA+ +G +S SC L A+ GTS S  + +GAAAMVRQYF D +FY  DV +RG CD GF C+SFSP + TVKA+LINSAN+MGG +EP+  RGFGR+H+EAGMPLGG GD+V+FVAD+++  +S+    ++ F+VD +AGL+LRAT+SW DPA+++ S  QLV+DLDL V +P GT + MW     D  NVNERVI+ A  V     +  W + V   +    D   +SLVV GAIS
Sbjct:    5 MGLVSFFASRHEVLKVTGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLEHGDGTEVEHGYYFDFVASSNPSFDSSSESRSMFKAEFTGGDFFYDSDRRKVIQYIDLINPDTGDG-------SSSGMPF--VSADSFVSDDDLGHGTHTATTVAGSTISSPAELVSCADNGEVIGCAGGCIIESSTGSTEADYGE----DINRLCPAFGCSG---DQCLPDDTANTLSEHGGMAQGAKIAAIDIFYGDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTD-RDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGNS-SCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYGADVESRGFCDQGFTCDSFSPMATTVKAMLINSANVMGGGNEPDSERGFGRIHLEAGMPLGGAGDMVLFVADSSDHEVSSNGKLKFNFNVDPDAGLELRATLSWSDPAASSLSGDQLVNDLDLRVYAPDGTRYCMWDDNCWDTSNVNERVIVPAEVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAIS 633