mRNA_A-nodosum_M_contig1112.4.1 (mRNA) Ascophyllum nodosum dioecious

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_A-nodosum_M_contig1112.4.1
Unique NamemRNA_A-nodosum_M_contig1112.4.1
TypemRNA
OrganismAscophyllum nodosum dioecious (Ascophyllum nodosum dioecious (Feamainn bhuX, rockweed, Norwegian kelp, knotted kelp, knotted wrack or egg wrack))
Homology
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BLAST of mRNA_A-nodosum_M_contig1112.4.1 vs. uniprot
Match: D7FRQ8_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FRQ8_ECTSI)

HSP 1 Score: 283 bits (725), Expect = 2.090e-82
Identity = 272/606 (44.88%), Postives = 313/606 (51.65%), Query Frame = 2 
Query:  200 ASELSELRAELWRTRDNLERMKSH-TPPSAGQSSHRLFNRRHH--------ADGNIVDRENAEPQ----RPNERDRVKDLKLDRCA----GGGVRSGPTS--------PPSG----------------------------GSSRSDQNNGRSGDGIAAGRGFSEEMAAEETLNSLALEGAAFKNWTEEASSIAVRLIEEVKTLRWERVTWTEREAATERA-----------ATELKERTXXXXXRLLAAEEDARRTKEALLRXXDGXXXXXXXXXRAEQDLRAARKRSEERWGRADGLEGRDTQVGRS------------------------------------LREELRREKEGADALQRRLDQARAASARHEVEKKEWHASRERLEELRRLWGKQLRKVEKKVRTMAKENKALQERVAEADKARECAEADARRSADELRRLTEQRDNLLLGATRAGH---LSASSPADALAELLAXXXXXXXXXXXXXXXXXXXXSSAHARGSVKGGAWGWADKYSDGAGGDSRAEDLEAELERARAEAKLARVAVKQERLRREGAAKEKDEAVRQLRRLMGAAKRAVSRKDARLRSSRMETEVVWNALTEAVR 1708
            ASELSELRAELWRTRD+LER+KS  TPPSA  S  R F+RR H        A     +  N +      R +E+ +   L+    A    GGG+RS P          P SG                            GSSR    +  S    AA  G SEE+AAEETL +L LEGAAFKNWTEEASSIAVRLIEEVK LRWE+ +W ERE A E             A  L+ER       L  AE D RR +EAL R              A+QD+             A+GL                                              L E LRREKEGADALQRRLD ARAASARHE E+KEW +S                        M KEN AL+ RV EA++AR+ A+AD+    +ELRRLTEQRDNLLLGATR G    L ASSPA+ LAELLA XXXXXXX                             D    G G D    +LEA+LE+AR+EAKLARVA +QERLRRE AA+EK+EAVRQL RLMGAA+RAV R+D+RL+SSRMETEVVWNALTEAVR
Sbjct:    3 ASELSELRAELWRTRDDLERIKSRGTPPSADCSPRRHFDRRRHRASPSPTLAATTACEVRNKDRDYRRHRHSEQHKGDGLEAGGGARGDSGGGIRSXPXXXXXXXXXXPGSGADYDDEAPRGLGGTASSGSSHGGRQLKFGSSRGRVGSASSEGENAAKGGSSEEVAAEETLCNLELEGAAFKNWTEEASSIAVRLIEEVKMLRWEKASWAERERAAEXXXXXXXXXXXXQADHLEER-------LGLAEADGRRAQEALAR--------------AQQDVERXXXXXXXXXXXAEGLRAAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLVEMLRREKEGADALQRRLDAARAASARHEKERKEWRSSXXXXXXXXXXXXXXXXXXXXXXXXMTKENAALRARVGEAERARDRADADSLGHREELRRLTEQRDNLLLGATRLGGRRGLVASSPAETLAELLAAXXXXXXX-----------------------------DATDVGPGADG---ELEAQLEQARSEAKLARVAAQQERLRREAAAREKEEAVRQLHRLMGAARRAVGRRDSRLKSSRMETEVVWNALTEAVR 555
The following BLAST results are available for this feature:
BLAST of mRNA_A-nodosum_M_contig1112.4.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 1
Match NameE-valueIdentityDescription
D7FRQ8_ECTSI2.090e-8244.88Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
A-nodosum_M_contig1112contigA-nodosum_M_contig1112:178898..184252 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ascophyllum nodosum dioecious OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Model size2439
Hectar predicted targeting categoryother localisation
Exons3
Ec32 ortholog descriptionexpressed unknown protein
Ec32 orthologEc-00_003480.1
Cds size1515
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680866507.4900305-UTR-A-nodosum_M_contig1112:178897..1789771680866507.4900305-UTR-A-nodosum_M_contig1112:178897..178977Ascophyllum nodosum dioeciousUTRA-nodosum_M_contig1112 178898..178977 +
1680866507.5031216-UTR-A-nodosum_M_contig1112:181672..1817881680866507.5031216-UTR-A-nodosum_M_contig1112:181672..181788Ascophyllum nodosum dioeciousUTRA-nodosum_M_contig1112 181673..181788 +
1680866507.5343597-UTR-A-nodosum_M_contig1112:183524..1842521680866507.5343597-UTR-A-nodosum_M_contig1112:183524..184252Ascophyllum nodosum dioeciousUTRA-nodosum_M_contig1112 183525..184252 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1680866507.5128806-CDS-A-nodosum_M_contig1112:181788..1826911680866507.5128806-CDS-A-nodosum_M_contig1112:181788..182691Ascophyllum nodosum dioeciousCDSA-nodosum_M_contig1112 181789..182691 +
1680866507.5231462-CDS-A-nodosum_M_contig1112:182912..1835241680866507.5231462-CDS-A-nodosum_M_contig1112:182912..183524Ascophyllum nodosum dioeciousCDSA-nodosum_M_contig1112 182913..183524 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_A-nodosum_M_contig1112.4.1prot_A-nodosum_M_contig1112.4.1Ascophyllum nodosum dioeciouspolypeptideA-nodosum_M_contig1112 181789..183524 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_A-nodosum_M_contig1112.4.1

>prot_A-nodosum_M_contig1112.4.1 ID=prot_A-nodosum_M_contig1112.4.1|Name=mRNA_A-nodosum_M_contig1112.4.1|organism=Ascophyllum nodosum dioecious|type=polypeptide|length=505bp
MASELSELRAELWRTRDNLERMKSHTPPSAGQSSHRLFNRRHHADGNIVD
RENAEPQRPNERDRVKDLKLDRCAGGGVRSGPTSPPSGGSSRSDQNNGRS
GDGIAAGRGFSEEMAAEETLNSLALEGAAFKNWTEEASSIAVRLIEEVKT
LRWERVTWTEREAATERAATELKERTEALERRLLAAEEDARRTKEALLRA
QDGEREQARATERAEQDLRAARKRSEERWGRADGLEGRDTQVGRSLREEL
RREKEGADALQRRLDQARAASARHEVEKKEWHASRERLEELRRLWGKQLR
KVEKKVRTMAKENKALQERVAEADKARECAEADARRSADELRRLTEQRDN
LLLGATRAGHLSASSPADALAELLAAAAAGVVAGAGGDDHAAARRSSAHA
RGSVKGGAWGWADKYSDGAGGDSRAEDLEAELERARAEAKLARVAVKQER
LRREGAAKEKDEAVRQLRRLMGAAKRAVSRKDARLRSSRMETEVVWNALT
EAVR*
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mRNA from alignment at A-nodosum_M_contig1112:178898..184252+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_A-nodosum_M_contig1112.4.1 ID=mRNA_A-nodosum_M_contig1112.4.1|Name=mRNA_A-nodosum_M_contig1112.4.1|organism=Ascophyllum nodosum dioecious|type=mRNA|length=5355bp|location=Sequence derived from alignment at A-nodosum_M_contig1112:178898..184252+ (Ascophyllum nodosum dioecious)
GTAGCGCGGAAACTAAGTACTTTTCACTTGGGAGGAGGTGCTGATCTACC
TCTGTTTACGGTCATTTTCTTGAAAGACGGGTGAGTCGGTTGGGGCTGAC
GACACATGTCACCTTCCTCAAGCCAGCTATCCTACTTTTCCCCTTGTACT
AGGCGTGACGGCTCCAAACAACTGAGTCGCCGATTGTCCACCGACATCTC
GTGACGGCTGACTTGCTATTACTACGAATACATCGTACTTGAAGAGCTAA
CTTTAAAGAACAAACTTCCAACCGGGGATATGTCGTTCTCGTTGACTGAT
ATCCGGTAGACGAATGAAGCAGCTTGTCTTGGCGTATTTTGAGACCCCGA
TAAAATCATTTGTATGTCCATGTCGCCTTGCACGTTGATGAACTTCCGAA
CTGATAAACATAATCGTATGGGGGCCGGTCGCATGAAATGCTTGATGCTG
TACAGTAGCTCTTGATGTTGTAGCTCTACCCTACCGACCATCTTCTTCGT
TTCTGTCAACAAGTAGTGTACGTATGAAACCTCAGTGCGTAAAACGAAAA
GTTGGCCGATTAGACTACTACAAACTACAACTGTTGTAATAGACACAATG
CGAAAATACTATGGTTACGTGCAGACAATGTACTGTCCTGTGGGAGACTA
TCTTGAAGCTCGAGCTCGGAGCGAGGGTTCCAGGCGATGCCCTTCCCCTA
TACCTTCACTTCTATGGCGTCGCTCTTCGACATCAGGGATAGTGTGAGGA
TATTCCGACGCCCTTTGTGTGTCTGAGGTCTCCTCTCGTTGATGTTTGAT
ACTTCGAATCCCGCGCTTCGGCTCCGTCCTGGCGCTGGGTATTTCCACCC
AAATCAGTTGTGAGACATTCTTTATCGCACATGTCCACCCGATACTTTCT
GTATCACACATTATTATAACATTATAACATGTACACTATACACCAACAGT
GGGTGTGGGAAAAGAGAGGTATAAAAAATGATCCATGGGTATACCTGAAA
AGGCAGCACCCGTTACTCGAACTACCTTGAGAACTGCTGGCCCTGTGCCG
GCGTACTCTTGGCAGTGAACGCCATCGGTGAGCACTTGCGTGACCTGATA
AACTCTGGACTGACCCGATGGCGGTTGACGGTGCAAATAAATAGACGCCG
CCGCGGAAAGCGGGAGGAATCCCGTGCGGAAGCACCATGTTCAGCCTGAG
TGTAGGGGAGTGGGCGGGCTGACGCGGGACGGAACGGCCGAATCTGTCTC
GCGAGACCAAATTCTCACGCGCGAACGGGGACAGAGAAAAAAATGAAATT
CATTTTCACCCGATTGATCCTTATTCTGCTATAAGTGATGACCGTACATA
CATACTCCCAGTAGCGTAGCTCCTCTCTTTCGACTCGAATAAAATACATG
CAAGCTTCCAGAACGAGCCTAGTACGACCGTATCTTTTCAATTTGAAACG
ACATTCGAGCATCAACTTTAAGTGCTTCTTCTCTGTTGAGAGGGGCGCAT
AGGTCATCTATTATTAGCAACCTCGAACATTCAGCCGATCTGTACGAAGG
GGCGTATGGATCCTCTACTGGTAGCATCCTTTAAAAATTCAGCCGACAGG
CACACCACGGAATAACAGTTGTTGTTGTTGTTGTCATCCCATGTGCTGTA
TGCGTTAAAATATATCTCTCGATACGTTTGTAAACACGCTCCAGCCGGGG
TCACAACAGGAGAAAGGCCACACATCCTTCCTACTGCGGTACATGCCTTA
GTTTTTTATCGCGAGAAGGGTTCAGCCCTCCCTTTCCCTCGTCGACGTTG
AAGTCGAATTTTGTGTACCCACGACAATTTAGTCGCTCTCCACGTGTTGG
GCATGATATGAGTTAGAATCCCAGTTCGTGTGACTGCAGCTAGGATTCGG
GCTCAATCCCCAACTTCAGATGGTTGCGGTTACCAACTGAAAGGTCGTTG
TCATCATCCCTTTGTCTGCGCGTCGTACAATATCTCTCGATACATCGGTA
GACACTCCAGTCGGGATAGCAACGTAACCTATTGTTCCTAATATTCCAGT
TCCAGCGGTGCCATTGGTGCACATTTTTTTGCACAACGGCGACAGAAGTA
TCTGGAAACTTTTCTGCGACGGGCGGTGTGTTTAGGACACCTGTACCACT
ATCTGTCCTTTACCTATAATAACTCCTCCTGCTGTCACAAAAGTAACACG
TCAGTGTGAAATACAAACACGTACCAGGTACAGTACTGAAAATATGGCAT
GGTTAAAAGTCCCTATTTACTTGTTGTAGAACGAAAACTTACCACTCTTT
ACCAATTATAGTATAATAACGTTCCTTATGTTCTAGACTATAACCGCCTA
ATGTGAAATGGTTCGATGGCACTATTATGGTATATTATTACTAACCTATT
TAATTACGTACAACATTGTTTTGTTTTGTTTTGTTTGGTGTCCCTGCATG
GCGATTAATGTAAGTGTACAGTATAACGGTGGGCTTCTCCCCGACATCAT
ACTGTTGACCCAATATTTCTACCACCGGGGAACCCGTTTAAATGCCACGA
AGAGGTTTGGTCTTTGCAGCCTATGATCCCACCTAAACATTTTGACATTT
TGCAGGGATGCTCAGAAGGTCTAAGATGCGTTCGAATTTTCCTTTAAACA
GTACAGTAAACACAACAGTACCTAATGATCCGACGTGTGACCAGTGGATG
CTCTCCGCTCACGGGATGACTTCCCGCGCTTGATCTCGCGCCTCGTCCCT
GAAACCCCGATTCAATCTGGATTAGCCAAGTCCGACACGCCCAACAGCAT
TGTTCTACCACTCCTGACGGAGACTGAGCATTCTCGGGGCAACACGTTCT
CAACCGAACTTTGGTCTACGTTGCCTTCCTTGGCGCGGAACATGGCTTCG
GAGTTGTCGGAGCTGCGCGCCGAGCTCTGGCGGACGCGTGACAACCTCGA
GCGCATGAAGTCTCACACCCCACCCAGCGCCGGGCAATCATCGCACCGCC
TGTTCAATCGCCGTCATCACGCAGACGGTAATATCGTGGACAGAGAGAAC
GCTGAACCACAACGTCCGAACGAGCGGGACAGGGTCAAGGATCTCAAGCT
CGATCGCTGCGCTGGCGGCGGGGTGAGGAGTGGCCCCACCAGCCCGCCAA
GCGGGGGGTCCTCCAGATCCGATCAAAACAACGGCCGCAGCGGCGACGGT
ATTGCTGCCGGTCGTGGTTTCAGCGAGGAGATGGCGGCCGAGGAAACACT
TAATAGCTTAGCGCTCGAGGGTGCCGCGTTCAAGAACTGGACGGAGGAGG
CGTCATCGATCGCCGTCCGCCTCATCGAAGAGGTTAAGACATTGCGCTGG
GAAAGGGTGACTTGGACTGAAAGAGAAGCCGCGACCGAGCGTGCGGCCAC
CGAGCTCAAAGAGCGAACCGAGGCTCTCGAGCGACGGCTTCTCGCGGCCG
AGGAGGACGCGCGGCGGACGAAGGAGGCGTTACTGAGGGCACAGGATGGA
GAGAGAGAGCAGGCAAGGGCGACGGAGCGAGCGGAGCAGGACCTGCGAGC
GGCCCGAAAAAGGTCGGAGGAGCGTTGGGGCCGGGCGGACGGGTTGGAGG
GTCGCGACACGCAAGTCGGGAGAAGCCTACGAGAAGAGCTTCGTCGTGAG
AAGGAAGGTGCCGACGCGCTGCAGCGGAGGCTGGATCAGGCGAGGGCGGC
GTCAGCGAGGCATGAGGTCGAGAAGAAGGAGTGGCACGCATCGAGGGAAA
GACTCGAAGAGCTTCGTCGCTTGTGGGGGAAGCAGCTGAGGAAGGCATGC
TTACTTTTCGTATTTCCACTGTGCCCTGTGCAAATTCTAGTGGATGTCTG
TAATGCGCTCGTGGCGTATTATGCTATGTCTTAGGTGCTTCGGATGTGAT
TGAGAATGATACCTCAGGGGCAACTCACGCCCGGATCGTGCTCGTAAGTG
TAATAACGCACGTATCACAGTGATTCTCTTCATACCTCCCTATCCATGCG
GCTCCGGTTTCTCAGGTGGAGAAGAAGGTGCGAACGATGGCTAAAGAGAA
CAAAGCACTTCAGGAAAGAGTCGCCGAGGCGGACAAGGCGAGAGAATGCG
CCGAGGCAGACGCGCGCCGCAGCGCCGACGAGCTGCGGCGACTCACCGAG
CAGCGCGATAACCTTCTGCTGGGAGCCACCCGCGCGGGACACTTGTCCGC
TTCGTCCCCCGCAGACGCGCTGGCAGAACTCCTCGCGGCAGCAGCGGCGG
GCGTTGTTGCGGGGGCTGGGGGTGACGACCATGCTGCGGCGCGTAGATCG
TCAGCGCACGCGCGAGGTTCTGTAAAAGGAGGGGCTTGGGGGTGGGCAGA
TAAGTACAGTGATGGCGCCGGGGGCGACAGCAGGGCGGAGGATCTCGAGG
CTGAGCTGGAGCGGGCCAGGGCCGAGGCCAAGCTGGCGAGGGTGGCGGTA
AAGCAGGAGCGGCTCCGGCGGGAGGGAGCGGCGAAGGAGAAAGATGAGGC
GGTGCGACAGTTACGTAGGTTAATGGGCGCGGCGAAGAGGGCGGTGAGCA
GGAAAGACGCCCGACTACGGTCTAGCCGAATGGAGACGGAGGTGGTGTGG
AACGCGCTGACGGAGGCCGTACGCTGACACCCCCCCTAGAAGTATTTTCT
GAGGCCATTTTTACAACCCTCACGTAACTTCGGCAGTCATCGGCAGTCAT
CGTAACCAGCACAATTACTTGGTATTAGAAGGGCAAGCTTTCCGCTGAGA
TGCGACTCGGTGCCTGCGCTCTGTTCTCTACTTTTGCCATGCGCTACCAG
CGGCTAGAACGTATGGATCACACGAAGCGAATCGGCGGCGAAATGCAAGT
GTGACGAACGATCCTTCACCGACGAAGAAACTATTGACCGTGGTTGCAAC
GCAGGAGCAGCTATCGGCTATTTAGCATTCCCTCGAGAACATGCACCACA
GCAAAACGTTTGAGCAGAATCCGTACAAAGCGCCTATGAATAGCTTGTGG
TAGTGGGGTCGCCACAAGAGGTGCTCACGACAGATCATTAGATTTACGAG
TCTGTAAGCTACACGCGATCCACACGTCCACAAGTAAGGGTCGATTTTGA
GCCCTCTCCCTCTAACTTTCCAATTTTAAGAAAAAAAACTTACGGAAACA
GTTCGGAAGTGGTCGTTCCGTTTTTTGTTTTTGTTTTCTGTCCTTTTCCA
GAGTTGGCAAATCTTTTTAGGATTCGGGATAGGTTTTGGGGAGTTTAAGT
ACGCGGGTAAAATGTGATTGATGGTGCACAGCAGTGCAATGTTTCGCAGG
TCATAGCGGTGATATTGATGGCATATACAGGCACTCCTGCTCCGCGGGGT
TCGGG
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Coding sequence (CDS) from alignment at A-nodosum_M_contig1112:178898..184252+

>mRNA_A-nodosum_M_contig1112.4.1 ID=mRNA_A-nodosum_M_contig1112.4.1|Name=mRNA_A-nodosum_M_contig1112.4.1|organism=Ascophyllum nodosum dioecious|type=CDS|length=1515bp|location=Sequence derived from alignment at A-nodosum_M_contig1112:178898..184252+ (Ascophyllum nodosum dioecious)
ATGGCTTCGGAGTTGTCGGAGCTGCGCGCCGAGCTCTGGCGGACGCGTGA
CAACCTCGAGCGCATGAAGTCTCACACCCCACCCAGCGCCGGGCAATCAT
CGCACCGCCTGTTCAATCGCCGTCATCACGCAGACGGTAATATCGTGGAC
AGAGAGAACGCTGAACCACAACGTCCGAACGAGCGGGACAGGGTCAAGGA
TCTCAAGCTCGATCGCTGCGCTGGCGGCGGGGTGAGGAGTGGCCCCACCA
GCCCGCCAAGCGGGGGGTCCTCCAGATCCGATCAAAACAACGGCCGCAGC
GGCGACGGTATTGCTGCCGGTCGTGGTTTCAGCGAGGAGATGGCGGCCGA
GGAAACACTTAATAGCTTAGCGCTCGAGGGTGCCGCGTTCAAGAACTGGA
CGGAGGAGGCGTCATCGATCGCCGTCCGCCTCATCGAAGAGGTTAAGACA
TTGCGCTGGGAAAGGGTGACTTGGACTGAAAGAGAAGCCGCGACCGAGCG
TGCGGCCACCGAGCTCAAAGAGCGAACCGAGGCTCTCGAGCGACGGCTTC
TCGCGGCCGAGGAGGACGCGCGGCGGACGAAGGAGGCGTTACTGAGGGCA
CAGGATGGAGAGAGAGAGCAGGCAAGGGCGACGGAGCGAGCGGAGCAGGA
CCTGCGAGCGGCCCGAAAAAGGTCGGAGGAGCGTTGGGGCCGGGCGGACG
GGTTGGAGGGTCGCGACACGCAAGTCGGGAGAAGCCTACGAGAAGAGCTT
CGTCGTGAGAAGGAAGGTGCCGACGCGCTGCAGCGGAGGCTGGATCAGGC
GAGGGCGGCGTCAGCGAGGCATGAGGTCGAGAAGAAGGAGTGGCACGCAT
CGAGGGAAAGACTCGAAGAGCTTCGTCGCTTGTGGGGGAAGCAGCTGAGG
AAGGTGGAGAAGAAGGTGCGAACGATGGCTAAAGAGAACAAAGCACTTCA
GGAAAGAGTCGCCGAGGCGGACAAGGCGAGAGAATGCGCCGAGGCAGACG
CGCGCCGCAGCGCCGACGAGCTGCGGCGACTCACCGAGCAGCGCGATAAC
CTTCTGCTGGGAGCCACCCGCGCGGGACACTTGTCCGCTTCGTCCCCCGC
AGACGCGCTGGCAGAACTCCTCGCGGCAGCAGCGGCGGGCGTTGTTGCGG
GGGCTGGGGGTGACGACCATGCTGCGGCGCGTAGATCGTCAGCGCACGCG
CGAGGTTCTGTAAAAGGAGGGGCTTGGGGGTGGGCAGATAAGTACAGTGA
TGGCGCCGGGGGCGACAGCAGGGCGGAGGATCTCGAGGCTGAGCTGGAGC
GGGCCAGGGCCGAGGCCAAGCTGGCGAGGGTGGCGGTAAAGCAGGAGCGG
CTCCGGCGGGAGGGAGCGGCGAAGGAGAAAGATGAGGCGGTGCGACAGTT
ACGTAGGTTAATGGGCGCGGCGAAGAGGGCGGTGAGCAGGAAAGACGCCC
GACTACGGTCTAGCCGAATGGAGACGGAGGTGGTGTGGAACGCGCTGACG
GAGGCCGTACGCTGA
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