mRNA_A-nodosum_M_contig8530.1.1 (mRNA) Ascophyllum nodosum dioecious

BLAST of mRNA_A-nodosum_M_contig8530.1.1 vs. uniprot
Match: D8LE49_ECTSI (Purple acid phosphatase n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LE49_ECTSI)

HSP 1 Score: 802 bits (2072), Expect = 3.740e-283
Identity = 417/723 (57.68%), Postives = 500/723 (69.16%), Query Frame = 1

Query:   28 HTGPALLDAFKPPRVELDPDITVSVSPTMLMKSGEWVTIMYTGIESWRFPDAFVAAFSPGGVLDDPATITQVAPIKYQYLTAERPLLGDADAILDGKAGEEAGAGENQGELSTVQTLRFRLLNMRDPEGYSFGLFKGGIENPVLVAKTEEPVMFARPYEVMHLHLALTAEANSMRVSWVTGAASQSPAVRYRQVTGGAGS---------WEEVAGET-LTYTREDMCGAPATTKGFHDPGLQHSAILTGLAPGMGYEYQAGDKEAAEWSESTFF-----------------------------FAPP-------------------------ESLAKSAGDREAISSGE---WGPEVVKMGVFGDMGTAEVDGTLDAGHSEEVPSLQTVAHLKEQL--GVSLQLAAAEGGALGRDPR-----QEPPLSLVLHIGDLSYARGYDAQWDEYLDQIAPIASAVPWMVGVGNHERD--STAISPIRPELPYYTGTDSGGDCGVPTAFRFLMPGDAGKPLDDKPWYGFDHGPVHFTIMSTEHDFTPGSTQYLFIEEDLAGVDRTKTPWLVFAGHRPMYVNSGGPGASDCEGPATEGEHCATDQPVARLMRVSLEPLLLQYQVDLSVYGHHHSYQRTCKVADMVCKGVSTRSSEGGAE------GYVAPVHVVLGMAGMGLSQNMVSPPPEWLEYATDREFGLCTLVADRSKLQLSFILNSDGQVSSRV 1950
            HTGP+ L A +PP   LDPDIT+ V PT+L +SG+WVT+ ++GIESW FPDAFVAAFSPG  LD PAT+ +VAPIKYQ+LTAE+P  G          G EA       E   V++LRFRLLN+RD EGY FGLFKGG+E+PVLVA+T E V FA+P+EV+HLHLALT++ +SMRVSWVTG ASQ+PAV +R+V  GA           W+EVA E+ +TY REDMCG PAT+ GFH+PGL HSA+L GL PG  YEY+AGD +A EW  S+F                              F+PP                          +LA S  D  +    E   W P+ VK+ VFGDMGTAE+DGTLDAGH+ E PS++TV  L + L  G  ++   + GG  G         +EP L LVLHIGDLSYARGYDAQWDEY+DQI  +AS VPWMVGVGNHERD  +T+ SP+R EL ++TGTDSGGDCGVPTAFRF+MPG A +P  D PWYGFD GPVHFT+MSTEH+F+ GS QY FI+EDLAGVDR KTPW+VF+GHRPMYVNSGG GA +CEG A    +CA DQPVAR +R +LEPLL++YQVDL+VYGHHHSYQRTC+VA+  C G S+R+     +       Y APVHVV+GMAGMGLSQNMVSP PEW+EYATDREFGL  +VAD SKLQLSFIL++DGQV   V
Sbjct:   21 HTGPSKLSAIQPPTFALDPDITLEVGPTLLFESGQWVTVSWSGIESWMFPDAFVAAFSPGTALDYPATVKEVAPIKYQFLTAEKPFPG---------VGHEA-------ETGAVESLRFRLLNLRDAEGYRFGLFKGGVEDPVLVARTTEAVTFAQPFEVLHLHLALTSDVDSMRVSWVTGEASQAPAVMFREVAVGAQEGVTETQVDPWQEVAAESSITYGREDMCGEPATSNGFHNPGLLHSAVLPGLIPGHPYEYKAGDSDAQEWGSSSFXXXXXXXXXXXXXXXXRAGSPEQPSSAGAAFSPPLVNTVTASGDASTADMSGKGSRPEMAALAPSELDNASGIGAEGIAWDPDAVKVAVFGDMGTAELDGTLDAGHTSEPPSIRTVGILNDHLRGGAGVRAVGSSGGGDGVSTGPTGGGEEPQLGLVLHIGDLSYARGYDAQWDEYMDQIKHVASTVPWMVGVGNHERDYPTTSESPVRQELSFFTGTDSGGDCGVPTAFRFIMPGAAEEPTADCPWYGFDFGPVHFTVMSTEHNFSVGSKQYAFIKEDLAGVDRAKTPWIVFSGHRPMYVNSGGAGAGECEGAAALEPNCANDQPVARSLRAALEPLLIEYQVDLAVYGHHHSYQRTCRVANETCVGPSSRTYSSQYQEYQEHQDYTAPVHVVMGMAGMGLSQNMVSPRPEWVEYATDREFGLGMIVADSSKLQLSFILDADGQVGDEV 727          

BLAST of mRNA_A-nodosum_M_contig8530.1.1 vs. uniprot
Match: A0A7S2XV45_9STRA (Purple acid phosphatase n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2XV45_9STRA)

HSP 1 Score: 408 bits (1049), Expect = 8.690e-131
Identity = 261/686 (38.05%), Postives = 353/686 (51.46%), Query Frame = 1

Query:   70 VELDPDITVSVSPTMLMKSGEWVTIMYTGIESWRFPDAFVAAFSPGGVLDDPATIT---------QVAPIKYQYLTAERPLLGDADAILDGKAGEEAGAGENQGELSTVQTLRFRLLNMRDPEGYSFGLFKGGIENPVLVAKTEEPVMFARPYEVMHLHLALTAEANS---------------MRVSWVTGAASQSPAVRYRQVTGGAGSWEEVAG------------ETLTYTREDMCGAPATTKGFH-DPGLQHSAILTGLAPGMGYEYQAGDKEAAEWSESTFFFAPPESLAK-------------------SAGDREAISSGE----WGPEVVKMGVFGDMGTAEVDGT---LDA-GHSEEVPSLQTVAHLKEQLGVSLQLAAAEGGALGRDPRQEPPLSLVLHIGDLSYARGYDAQWDEYLDQIAPIASAVPWMVGVGNHERDSTAISPIRPELPYYTGTDSGGDCGVPTAFRFLMPGDAGKPLDDKPWYGFDHGPVHFTIMSTEHDFTPGSTQYLFIEEDLAGVDRTKTPWLVFAGHRPMYVNSGGPGASDCEGPATEGEHCATDQPVARLMRVSLEPLLLQYQVDLSVYGHHHSYQRTCKVADMVCKGVSTRSSEGGAEGYVAPVHVVLGMAGMGLSQNMVSPPPEWLEYATDREFGLCTLVADRSKLQLSFILNSDGQ 1935
            V +D  I   V P +L  SG+   I + G+    + D FVA +SP   L    +I           VAP+++Q+ +        +D  +D       G G+          +  R+LN+R  E +    F+GG+++P LVA    PV   +P EV  LHL+L +  +                MR +WVT      PAV ++++      W E  G            +T TY  ED+CG PA T+G+   PG  HSA+L GL P   Y Y+ GD +   WS+   F +PP SL                     S  D     +GE    WGP  V+  +FGD+G A  DG+   L+A G  EE+PSL+TV  ++++   S +                 P+  VLH+GDLSYA GY A WD +L  I P+AS VPWMV +GNHERDST  S I  E  +    DSGG+CGVPT   F MPG A    DD PWY F+ GPVH T++STEHDFT GS Q  +++ DL  VDR  TPWL+ AGHRPMY++SGG                 TDQ VA ++R SLEPLL +Y+VDL+ YGHHHSYQRTC V +  C  +    +        APVH+V+GMAG  LSQN  + PP WL Y+ D+E+G   +  +++ L L + +NSDG+
Sbjct:   26 VNVDSAIDFRVWPEILQYSGDSFKISWEGVWPSDYGD-FVAVYSPPSALYKSLSIDGSSEGQSGLDVAPVQFQFASV-------SDTHMD------LGHGD----------ITLRVLNLR--EDFIVAFFRGGLKSPELVA-VSNPVANVQPNEVTGLHLSLASPLSPSESAASAEEQGALGVMRATWVTRDTGN-PAVVWQELPNP--QWPESWGFSDAHPVEVSLAQTSTYYPEDLCGEPAKTRGYGAGPGSIHSALLRGLKPSTAYIYRVGDSDFNTWSDFRIFHSPPPSLYPVRWTLDQASPARTLREVDVSELDARHSMAGEVEDQWGPHSVRFSLFGDLGAAMEDGSHSGLEADGGKEEIPSLRTVGQIEDKHRYSSR-----------------PIHFVLHVGDLSYADGYQAHWDSFLHMIEPVASRVPWMVLMGNHERDSTTTSRIMKEPSFSGKDDSGGECGVPTGMYFPMPGPATNVADDSPWYSFEFGPVHITVVSTEHDFTSGSKQMAWLKNDLKSVDREATPWLLLAGHRPMYLDSGG----------------HTDQKVAVMLRGSLEPLLWKYRVDLAAYGHHHSYQRTCPVYEGQCYSLKDNVAS-------APVHMVVGMAGRTLSQNGAAIPPPWLSYSNDQEYGTTFVEVNKTHLHLEYFVNSDGK 641          

BLAST of mRNA_A-nodosum_M_contig8530.1.1 vs. uniprot
Match: W7TJW3_9STRA (Purple acid phosphatase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TJW3_9STRA)

HSP 1 Score: 405 bits (1042), Expect = 1.740e-128
Identity = 263/696 (37.79%), Postives = 367/696 (52.73%), Query Frame = 1

Query:   13 TSSRAHTGPALLDAFKPPRVELDPDITVSVSPTMLMKSGEWVTIMYTGIESWRFPDAFVAAFSPGGVLDDPATITQVAPIKYQYLTAER--PLLGDADAILDGKAGEEAGAGENQGELSTVQTLRFRLLNMRDPEGYSFGLFKGGIENPVLVAKTEEPVMFARPYEVMHLHLALTAEANSMRVSWVTGAASQSPAVRY-------------------RQVTGGAGS--W--EEVAGE------------------------------TLTYTREDMCGAPATTKGFHDPGLQHSAILTGLAPGMGYEYQAGDKEAAEWSESTFFFAPPESLAKSAGDREAISSGEWGPEVVKMGVFGDMGTAEVDGTLDAGHSEEVPSLQTVAHLKEQLGVSLQLAAAEGGALGRDPRQEPPLSLVLHIGDLSYARGYDAQWDEYLDQIAPIASAVPWMVGVGNHERDSTAISPIRPELPYYTGTDSGGDCGVPTAFRFLMPGDAGKPLDDKPWYGFDHGPVHFTIMSTEHDFTPGSTQYLFIEEDLAG-VDRTKTPWLVFAGHRPMYVNSGGPGASDCEGPATEGEHCATDQPVARLMRVSLEPLLLQYQVDLSVYGHHHSYQRTCKVADMVCKGVSTRSSEGGAEGYVAPVHVVLGMAGMGLSQNMVSPPPEWLEYATDREFGLCTLVADRSKLQLSFILNSDG 1932
            T+ R+ +G AL    K   ++   ++  +++P++L  SGE   +    + +      FVA ++P  V      +   APIK +++  E+  PLL  + A              + GE      L FRLLN+RD   Y+F L +G +  P+L+ ++  P+ F    EV  +HLALT     M VSWV+   ++ P V++                     + GG GS  W  +E A                                T TY+R D+C  PAT+ G+HDPG  H+ ++TGL  G+ Y Y+ GD +   WSE   F AP     + A + +         E     VFGDMGTAE DG+LD G  EEV SL T   L               GAL   P   PPLSLVLHIGDLSYA+GYD QWDE+L Q   I   VPWMV  GNHERD++ +S +      +TG DSGG+CGVPT  RF MPG   +   D+PWY F  GPVH+ ++ TE DF+PGS Q+ F+ + L+  V+RT TPW+V A HRP+Y++S      D +    +GE  ++DQ VARLM+ SLE +++QY+VDL +YGHHHSYQRTC+V    C   S+ +       Y APVHVV+GMAG GLSQN++   P   E   D+ FGL  +  +R+ L ++F+ +++G
Sbjct:   96 TAFRSVSG-ALSRERKEKNLDNAGEVRFNITPSVLETSGEAAQVCIKHVPNPDPRTDFVAVYAPADV-----NLQATAPIKIKFVGEEKSPPLLPSSYA--------------STGEAC----LSFRLLNLRD--SYTFALMRGNVSAPLLLGQSS-PLGFQEANEVTQIHLALTGIDGEMAVSWVSRD-TKKPLVQWWPAAPEEKSQDEGSGDAQAGAMDGGDGSVAWASKETASRCETQRRLKGAQNGAKAGVRPPMTQHSSLALTTTYSRGDLCSPPATSIGYHDPGALHTGVMTGLKGGVSYVYRVGDADLNVWSECITFKAPGYFSRREAEEGKV--------ETFTFAVFGDMGTAEEDGSLDIG-GEEVASLNTTRLLM--------------GALS--PSIHPPLSLVLHIGDLSYAKGYDYQWDEFLSQNQAIMKVVPWMVSSGNHERDASDVSRVHVSPSLFTGEDSGGECGVPTQLRFPMPGPWRELEKDEPWYDFSTGPVHWVVLCTEKDFSPGSAQFSFMIDALSNKVNRTLTPWVVVAAHRPLYIDS------DWD----DGEDASSDQAVARLMQQSLEDVMVQYKVDLGLYGHHHSYQRTCQVYRNKCLAASSDAV------YKAPVHVVVGMAGQGLSQNVLPNQPSIFEKVDDKHFGLTLMTVNRTHLTITFLSDAEG 722          

BLAST of mRNA_A-nodosum_M_contig8530.1.1 vs. uniprot
Match: A0A1X7UL29_AMPQE (Purple acid phosphatase n=3 Tax=Amphimedon queenslandica TaxID=400682 RepID=A0A1X7UL29_AMPQE)

HSP 1 Score: 372 bits (954), Expect = 9.570e-118
Identity = 237/628 (37.74%), Postives = 322/628 (51.27%), Query Frame = 1

Query:   70 VELDPDITVSVSPTMLMKSGEWVTIMYTGIESWRFPDAFVAAFSPGGVLDDPATITQVAPIKYQYLTAERPLLGDADAILDGKAGEEAGAGENQGELSTVQTLRFRLLNMRDPEGYSFGLFKGGIENPVLVAKTEEPVMFARPYEVMHLHLALTAEANSMRVSWVTGAASQSPAVRYRQVTGGAGSWEEV-------AGETLTYTREDMCGAPATTKGFHDPGLQHSAILTGLAPGMGYEYQAGDKEAAEWSESTFFFAPPESLAKSAGDREAISSGEWGPEVVKMGVFGDMGTAEVDGTLDAGHSEEVPSLQTVAHLKEQLGVSLQLAAAEGGALGRDPRQEPPLSLVLHIGDLSYARGYDAQWDEYLDQIAPIASAVPWMVGVGNHERDSTAISPIRPELPYYTGTDSGGDCGVPTAFRFLMPGDAGKPLDDKPWYGFDHGPVHFTIMSTEHDFTPGSTQYLFIEEDLAGVDRTKTPWLVFAGHRPMYVNSGGPGASDCEGPATEGEHCATDQPVARLMRVSLEPLLLQYQVDLSVYGHHHSYQRTCKVADMVCKGVSTRSSEGGAEGYVAPVHVVLGMAGMGLSQNMVSPPPEWLEYATDREFGLCTLVADRSKLQLSFILNSDG 1932
            V  DP + ++V+P  L KSG+WVT+ + G+      D       P G  ++    +++AP+KYQY                    +E+    + G+ S     + RL+N+R P  Y F L  GG   P LVA T + V F+ P E +  HLALT +  ++ ++W T   S  P V++         W+ +       A  +  YT +DMCG PATT G+ DPG+ H+A L+GL PG  Y YQ GD    EWS+   F  PP     ++               +    FGDMG A+VD TL   +           H +     +  L A E     RD        LVLHIGD+SYA GY   WDE+ D I PI+S VP+MV  GNHERD            YY GTDSGG+CGVP   RF MP    +P   + WYGF  G VHF +MSTE DFT  S QY ++++ L+ VDR+ TPWL+FAGHRPMY++S            T G   A+D  V++ ++ ++EPLLL+Y+VDL+ +GHHHSYQRTC VA  VC+   T           APVHVV+GMAG  LS N+    P+W+ +    ++G   +      L L +I  SDG
Sbjct:   33 VNTDPSVVITVTPNQLNKSGDWVTVAWDGVSHPADTDWIGVYAPPNG--EESIDPSKIAPVKYQYC-------------------KESSTHMSSGKGS----FKIRLVNVRTP--YVFALLTGGFNAPTLVA-TSKQVTFSSPNEPLQPHLALTNDPTTLLLTWSTRD-SHEPKVKF---------WQNMTTYIRIEAATSNKYTSKDMCGPPATTVGYIDPGMLHTAKLSGLTPGQEYNYQFGDDP--EWSQVFSFRMPPAPSPNAS---------------ITFIAFGDMGQAQVDDTLQPLY----------VHAEPPAVNNTNLMAKEVNE--RD--------LVLHIGDISYAIGYAGVWDEFFDLIQPISSRVPYMVCGGNHERDYPHSG------SYYEGTDSGGECGVPYEMRFQMP----RPDPKQHWYGFSLGSVHFVLMSTEIDFTVNSVQYNWLKDHLSSVDRSVTPWLIFAGHRPMYIDS------------TAGVQAASDLVVSKELQDNIEPLLLEYKVDLAFWGHHHSYQRTCPVAKKVCQDDGT-----------APVHVVIGMAGQSLSGNIQEKQPDWIRFVDVDDYGYTRISVSPLSLTLEYI-KSDG 551          

BLAST of mRNA_A-nodosum_M_contig8530.1.1 vs. uniprot
Match: A0A7S2UVV3_9STRA (Purple acid phosphatase n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2UVV3_9STRA)

HSP 1 Score: 368 bits (945), Expect = 5.070e-115
Identity = 249/668 (37.28%), Postives = 338/668 (50.60%), Query Frame = 1

Query:   67 RVELDPDITVS-VSPTMLMKSGEWVTIMYTGIESWRFPDAFVAAFSPGGVLDDPATITQVAPIKYQYLTAERPLLGDADAILDGKAGEEA-----------GAGENQGELSTVQTLRFRLLNMRDPEGYSFGLFKGGIENPVLVAKTEEPVMFARPYEVMHLHLALTAEAN-------SMRVSWVT-------------GAASQSPAVRYR---------------QVTGGA----GSWEEVA-GETLTYTREDMCGAPATTKGFHDPGLQHSAILTGLAPGMGYEYQAGDKEAAEWSESTFFFAPPESLAKSAGDREAISSGEWGPEVVKMGVFGDMGTAEVDGTLDAGHSEEVPSLQTVAHLKEQLGVSLQLAAAEGGALGRDPRQEPPLSLVLHIGDLSYARGYDAQWDEYLDQIAPIASAVPWMVGVGNHERDSTAISPIRPELPYYTGTDSGGDCGVPTAFRFLMPGDAGKPLDDKPWYGFDHGPVHFTIMSTEHDFTPGSTQYLFIEEDLAGVDRTKTPWLVFAGHRPMYVNSGGPGASDCEGPATEGEHCATDQPVARLMRVSLEPLLLQYQVDLSVYGHHHSYQRTCKVADMVCKGVSTRSSEGGAEGYVAPVHVVLGMAGMGLSQNMVSPPPEWLEYATDREFGLCTLVADRSKLQLSF 1914
            +V +D    +S VSP +   +G W  +       W F                     Q+AP     +    P     D + + + G+EA           G G N    S   ++ F LLN+R    Y FGLF+GG +NPVLVA   E ++  RP E  ++HLAL  E         +MRVSWVT             G    S AV  R               +V GGA     +   VA   + T++ +DMC APA+  GFH PG  HS +L  L PG  Y Y+ GD E+ EWSE   F  PP   A S    E         + V++ VFGDMG A +DG+ ++  +    +    AHL E L   L+  + +     R P     L  VLHIGD+SYARG    WD +L +I  +AS VPWMVG+GNHERDST +S     L ++   DSGG+CGVP    F MP  A     D PWY FD G +HFT+MSTEHDF  GS+Q+ ++++DL  V R  TPW+VFAGHRPMYV+S            T G    +D  V+ LM+  LEP+L+++QVD+ +YGHHHSYQRTC+V D  C+   T +SE     +VAPVH+V+GM G   S+   S P +W+++ ++ + G   + A+ + L   F
Sbjct:   58 KVHMDSQYALSIVSPEIADGAGTWAKV------GWSFHSEL-----------------QLAPDSSTIIALFSPPEALLDLVEESEGGKEAMRHPPVKAHHLGEGMN----SRSGSVDFLLLNLRTD--YIFGLFQGGSDNPVLVA-VSEVLLNGRPNEPNNIHLALADEGEGHGAGLAAMRVSWVTRDEGRPVVMFQEEGPQLASGAVAPRHLRSVRRLMSHVVGGKVLGGATLPTATKHSVAPASSFTFSAKDMCTAPASGAGFHSPGTLHSVVLANLTPGTRYRYRVGDLESQEWSEYRVFVTPP---APSYLPTE---------QSVRIAVFGDMGAAPIDGSYESTVTGSQWASLGTAHLLESL---LEKPSQQ---FSRSPSASG-LDAVLHIGDISYARGVTFHWDMFLQEIEQVASHVPWMVGIGNHERDSTDVSDTIKSLSFFFTDDSGGECGVPYGAYFPMPEPADDFKKDTPWYSFDFGAIHFTVMSTEHDFRRGSSQFQWLKQDLKDVHREVTPWVVFAGHRPMYVDS------------TWGGDFDSDVAVSDLMKQELEPMLMEHQVDIGMYGHHHSYQRTCQVFDGECQ--DTSASEPSV--FVAPVHLVVGMGGYDFSEFAESTP-DWIDFQSNEQHGASVIEANHTHLHFQF 659          

BLAST of mRNA_A-nodosum_M_contig8530.1.1 vs. uniprot
Match: L8H162_ACACA (Purple acid phosphatase n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8H162_ACACA)

HSP 1 Score: 354 bits (909), Expect = 5.870e-111
Identity = 231/595 (38.82%), Postives = 306/595 (51.43%), Query Frame = 1

Query:  157 IESWRFPDAFVAAFSPGGVLDDPATITQVAPIKYQYLTAERPLLGDADAILDGKAGEEAGAGENQGELST-VQTLRFRLLNMRDPEGYSFGLFKGGIENPVLVAKTEEPVMFARPYEVMHLHLALTAEANSMRVSWVTGAASQSPAVRYRQVTGGAGSWEEVAGETLTYTREDMCGAPATTKGFHDPGLQHSAILTGLAPGMGYEYQAGDKEAAEWSESTFFFAPPESLAKSAGDREAISSGEWGPEVVKMGVFGDMGTAEVDGTLDAGHSEEVPSLQTVAHLKEQLGVSLQLAAAEGGALGRDPRQEPPLSLVLHIGDLSYARGYDAQWDEYLDQIAPIASAVPWMVGVGNHERDSTAISPIRPELPYYTGTDSGGDCGVPTAFRFLMPGDAGKPLDDKPWYGFDHGPVHFTIMSTEHDFTPGSTQYLFIEEDLAGVDRTKTPWLVFAGHRPMYVNSGGPGASDCEGPATEGEHCATDQPVARLMRVSLEPLLLQYQVDLSVYGHHHSYQRTCKVADMVCKGVSTRSSEGGAEGYVAPVHVVLGMAGMGLSQNMVSPPPEWLEYATDREFGLCTLVADRSKLQLSFILNSDGQV 1938
            + SWR  D+  A    G      A++T   PIKY++                        A E+   LST   ++RFRL NMR    Y+F  F+ GI  P LVA T   V F    E M   L LT   N MRV W T  A + P V++    G     + V   T TY +E MCGAPA  +G+ DPGL HSA+L+ L P   Y Y  GD      +E++F   P         DR           V+ +  FGDMG    D + +   SE + S+ T   + + L                D R   P+ L+LHIGD++YA GY AQWDE+ DQ++ I++ +P+M  +GNHERD             Y G+DSGG+CGV    R+ MP     P  D+PWY FD+G +HFT MSTEHDF+ GS Q+ ++EEDL  VDR KTPW+VF+GHRPMY++S G            G+  A DQPVAR +R ++E LL +YQVDL+++GHHHSYQR+C V     KG    S         AP HVV+GMAG  L+ N+    P W     D+E G   L   RS+L++ FI + D +V
Sbjct:   66 VVSWRGVDSPSAGDWIGVYSPANASVTTSVPIKYKF------------------------ADESTNYLSTGAGSVRFRLTNMR--ADYAFHFFRHGITRPTLVA-TSNAVTFVNYNEPMQGRLMLTGRQNEMRVMWTTRDAVR-PQVKFGTSPGNYD--QSVGAATSTYRKEHMCGAPANAEGWRDPGLLHSAVLSNLRPDTRYYYVYGDPTFGFSAEASFVSEPH----PGQSDR-----------VIHLFAFGDMGKTTQDNSTEHWDSE-LASINTTTLIAKDL----------------DAR---PMDLLLHIGDIAYAVGYGAQWDEFHDQVSAISTRLPYMTCIGNHERDFPNSG------SRYNGSDSGGECGVAYEARYPMP----TPARDQPWYSFDYGFIHFTFMSTEHDFSIGSVQWKWLEEDLKKVDRVKTPWVVFSGHRPMYIDSQG----------DIGD--AADQPVARELRANVEDLLFKYQVDLALWGHHHSYQRSCPVY----KGTCIPSGR-------APTHVVIGMAGFSLTTNLELEKPTWARVVNDQEHGYTRLAVTRSRLEMEFISDVDTRV 562          

BLAST of mRNA_A-nodosum_M_contig8530.1.1 vs. uniprot
Match: A0A0D2U9F6_CAPO3 (Purple acid phosphatase n=1 Tax=Capsaspora owczarzaki (strain ATCC 30864) TaxID=595528 RepID=A0A0D2U9F6_CAPO3)

HSP 1 Score: 354 bits (908), Expect = 1.690e-110
Identity = 208/536 (38.81%), Postives = 290/536 (54.10%), Query Frame = 1

Query:  373 TLRFRLLNMRDPEGYSFGLFKGGIENPVLVAKTEEPVMFARPYEV-MHLHLALTAEANSMRVSWVTGAASQSPAVRYRQVTGGAGSWEEVAGETLTYTREDMCGAPATTKGFHDPGLQHSAILTGLAPGMGYEYQAGDKEAAEWSESTFFFAPPESLAKSAGDREAISSGEWGPEVVKMGVFGDMGTAEVDGTLDAGHSEEVPSLQTVAHLKEQLGVSLQLAAAEGGALGRDPRQEPPLSLVLHIGDLSYARGYDAQWDEYLDQIAPIASAVPWMVGVGNHERDSTAISPIRPELPYYTGTDSGGDCGVPTAFRFLMPGD-----AGKPLD-----DKPWYGFDHGPVHFTIMSTEHDFTPGSTQYLFIEEDLAGVDRTKTPWLVFAGHRPMYVNSGGPGASDCEGPATEGEHCATDQPVARLMRVSLEPLLLQYQVDLSVYGHHHSYQRTCKVADMVCKGVSTRSSEGGAEGYVAPVHVVLGMAGMGLSQNMVSPPPEWLEYATDREFGLCTLVADRSKLQLSFILNSDGQVSSR 1947
            +L F L+NMRD   + F  F+GG+  PV VA+T+ PV F   Y++ MH HLA+T   + M + W +  A+   A+     T    ++      T +Y+  DMCG PAT+ G+   GL H+ I TGL P   Y Y  GD      +  +F  AP      + GD            +V+  VFGDMG AE DG+ +     E PS+ T   +  +L                   +   +  V H GD+SYARGY + WD +  Q+ PIASAVP+++  GNHERD      + P      G DSGG+CGVP   RFLMPG      AG  +D     D PWY  ++GP+H T+MSTEHDF+ GSTQ  +IE+DLA VDR+ TPWL+FAGHRPMY++S            T+      DQPVA  +R  +EPLL +Y+ DL+++GHHHSYQR+C   ++ C       +      Y+ PV+VV+GMAG  LSQN+++  P W+    D+ +G   L AD++ L   FI+N+  Q+  +
Sbjct:  115 SLTFTLINMRDSNSFVF--FRGGLTAPVAVAQTD-PVEF-ESYDIPMHPHLAITDNPSEMSLMWTSRKAAMPIALLGTSTTSVTTTFNAT---TTSYSASDMCGEPATSYGYRPAGLIHTVIFTGLQPRTRYYYVFGDPSYGMSTIYSFVSAP------ARGDTS----------LVRWVVFGDMGRAERDGSNEY-QVYEPPSINTTDRIIAEL-------------------KRGDVDFVGHFGDISYARGYASDWDSFFAQVRPIASAVPYLIASGNHERDWNNSGALFP------GYDSGGECGVPYNARFLMPGSKPTSKAGVRMDGGIVKDSPWYSANYGPIHLTVMSTEHDFSAGSTQLAWIEQDLASVDRSVTPWLLFAGHRPMYIDS------------TDVSPVTGDQPVATALRQFVEPLLFKYRADLTMFGHHHSYQRSCPSLNLTCITTPQPPNAATPWSYLGPVNVVIGMAGQSLSQNLIAAQPSWVVAVNDQVYGYARLQADKTSLAFQFIINNSDQIGDQ 589          

BLAST of mRNA_A-nodosum_M_contig8530.1.1 vs. uniprot
Match: A0A388LFV4_CHABU (Purple acid phosphatase n=1 Tax=Chara braunii TaxID=69332 RepID=A0A388LFV4_CHABU)

HSP 1 Score: 352 bits (904), Expect = 1.560e-108
Identity = 237/686 (34.55%), Postives = 330/686 (48.10%), Query Frame = 1

Query:   76 LDPDITVSVSPTMLMKSGEWVTIMYTGIESWRFPDAFVAAFSPGGVLDDPATITQVAPIKYQYLTAERPLLGDADAILDGKAGEEAGAGENQGELSTVQTLRFRLLNMRDPEGYSFGLFKGGIENPVLVAKTEEPVMFARPYEVMHLHLALTAEANSMRVSWVTGAASQSPAVRYRQVTGGAGSWEEVAGETLTYTREDMCGAPATTKGFHDPGLQHSAILTGLAPGMGYEYQAGDKEAAEWSESTFFFAPPESLAKSAGDREAISSGEWGPE-VVKMGVFGDMGTAEVDGTLDAGHSEEVPSLQTVAHLKEQLGVSLQLAAAEGGALGRDPRQEPPLSLVLHIGDLSYARGYDAQWDEYLDQIAPIASAVPWMVGVGNHERDSTAISPIRPELPYYTGTDSGGDCGVPTAFRFLMP-GDAGKPLD------------------------------------------------------------DKPWYGFDHGPVHFTIMSTEHDFTPGSTQYLFIEEDLAGVDRTKTPWLVFAGHRPMYVNSGGPGASDCEGPATEGEHCATDQPVARLMRVSLEPLLLQYQVDLSVYGHHHSYQRTCKVADMVCKGVSTRSSEGGAEGYVAPVHVVLGMAGMGLSQNMVSPPPEWLEYATDREFGLCTLVADRSKLQLSFILNSDGQVSSR 1947
            L+  + +SVSP+ML++SGEWVT+ + G++     D ++A + P     DP+   +VAP+KY+ +    P     D +  G                   +++FRLLNMRD   Y F L +     PV+++++   V F+   E   +HLALT +   MRV+W T    ++P VR+    G   S       T TYTR +MCG PA + G+  PG  H+A++ GL  G    Y  GD   A  S    F+ PP                  GP+  V+M  FGD+G AEVDG+ D   S E PSL T   +                 +  D R++  L LVLH GD+SYARGY  QWD + D+I P+AS VP+MV VGNHERD      +      +   DSGG+CG+    R  MP      PL                                                             D PWY  D+GP+HF +MSTEHDF  GS Q+ F+E+DLA V+R +TPW++F+GHRPMYV+S            T+      D PVA  M+  LE LL+ Y+VDL+++GHHH+Y+RTC V    C   ++  S      + APVH+V+GMAG G   N+    PEW+EY    + G   L+ + + L   FI NS G++  R
Sbjct:  107 LEHGVEISVSPSMLLRSGEWVTVSWKGVKEPSVYD-WIAVYVPADA--DPS---KVAPVKYK-MACNHP-----DHVKYGSG-----------------SVQFRLLNMRDD--YRFVLVRNWGTKPVVISRSNT-VAFSNYDEPTQIHLALTGKQGEMRVTWTTRGRGRAPYVRWGMTAGDYTSSSPA--NTTTYTRTEMCGGPARSIGWVAPGYFHTAVMNGLTVGTNIFYSVGDDGYAH-SNPKSFYVPP----------------NVGPQSTVRMLAFGDLGQAEVDGSNDMAESME-PSLLTARRI------------IRDAYMDDDSRRQYGL-LVLHAGDISYARGYTCQWDVFFDEIEPVASRVPYMVSVGNHERDWPGSGSL------FQVEDSGGECGISYGRRLPMPLPSLPPPLSPLIVHPSSPVLRSRKLLSSSSSSSFSSFLTLFGMKETTDVLLEGEAEVESANNALKNAGADTPWYSVDYGPIHFVVMSTEHDFKAGSAQFSFLEQDLASVNRRRTPWVIFSGHRPMYVDS------------TDDRPVQGDNPVAEQMKRWLEELLMTYRVDLAMWGHHHTYERTCAVYRNKCVPPNSDGS------FNAPVHLVVGMAGAGTCDNVRPVTPEWIEYLNTEQHGYVRLMVNATHLGYEFIGNSRGEILDR 703          

BLAST of mRNA_A-nodosum_M_contig8530.1.1 vs. uniprot
Match: L8H124_ACACA (Purple acid phosphatase n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8H124_ACACA)

HSP 1 Score: 341 bits (874), Expect = 7.560e-106
Identity = 241/665 (36.24%), Postives = 324/665 (48.72%), Query Frame = 1

Query:    1 MVLPTSSRAHTGPALLDAFKPPRVELDP-------DITVSVSPTMLMKSGEWVTIMYTGIESWRFPDAFVAAFSPGGVLDDPATITQVAPIKYQYLTAERPLLGDADAILDGKAGEEAGAGENQGELSTVQTLRFRLLNMRDPEGYSFGLFKGGIENPVLVAKTEEPVMFARPYEVMHLHLALTAEANSMRVSWVTGAASQSPAVRYRQVTGG-AGSWEEVAGETLTYTREDMCGAPATTKGFHDPGLQHSAILTGLAPGMGYEYQAGDKEAAEWSESTFFFAPPESLAKSAGDREAISSGEWGPEVVKMGVFGDMGTAEVDGTLDAGHSEEVPSLQTVAHLKEQLGVSLQLAAAEGGALGRDPRQEPPLSLVLHIGDLSYARGYDAQWDEYLDQIAPIASAVPWMVGVGNHERDSTAISPIRPELPYYTGTDSGGDCGVPTAFRFLMPGDAGKPLDDKPWYGFDHGPVHFTIMSTEHDFTPGSTQYLFIEEDLAGVDRTKTPWLVFAGHRPMYVNSGGPGASDCEGPATEGEHCATDQPVARLMRVSLEPLLLQYQVDLSVYGHHHS-----------YQRTCKVADMVCKGVSTRSSEGGAEGYVAPVHVVLGMAGMGLSQNMVSPPPEWLEYATDREFGLCTLVADRSKLQLSFILNSDGQV 1938
            +VLPTSS    G    DA    R+ LD        D+ +S  P  L +SGEWV++ ++           V  +SP       A +T  APIKY+Y          AD   DG                    L FRL NMR    Y F  F GGI+ P L+A +   V FA   E +   L LT   + MRV W T   + SP V++    G   GS   V+  T TYTR+ MCGAPA T+G+ DPGL HSA+L+ L+P   Y Y  GD       E++F  AP    A                  + +  +GDMG      T    ++E+     T   +K+   +                    P+ L +HIGD+SYA GY AQWDE+ DQ++ I++ +P+M  +GNHERD             + GTDSGG+CGV    R+ MP     P  D+PWY FD+G VHF  MS+EH+FT G TQ+ +IE DL  VDRTKTPW++F+GHRPMY++S             +G+  + DQPVAR +R +LE LL +Y+VDL+ +GHHHS           YQR+C V +  C       SEG      A  HVV+GMAG  LS ++    P W       E G   L    S+L+++FI + D +V
Sbjct:    8 VVLPTSSAHIHG---RDAGASTRIHLDKIVVARDGDVRMSAWPGELKRSGEWVSVSWSXXXXXXXXX--VGVYSPAD-----ADVTVTAPIKYKY----------ADEFKDGYG-----------------KLWFRLTNMR--ADYVFHYFSGGIDKPTLIASSNR-VTFANYNEPLQGRLMLTGVPHEMRVMWTT-LNTTSPQVKFGTSPGQYVGS---VSASTTTYTRDQMCGAPANTEGWRDPGLFHSAVLSNLSPDTRYYYVYGDPAYGFSEEASFMSAPRPGAAS---------------RTLNIFAYGDMGK-----TTQHWNNEKASINTTRLMIKDMQAI--------------------PMDLAIHIGDISYAVGYGAQWDEFHDQVSAISTRLPYMTCIGNHERDFPNSG------SRFNGTDSGGECGVAYEVRYPMP----TPGRDQPWYSFDYGSVHFVFMSSEHNFTIGGTQWQWIEADLRKVDRTKTPWIIFSGHRPMYIDSN----------YDKGD--SADQPVARELRRNLEDLLFKYRVDLAFWGHHHSSVESCLLVGAQYQRSCPVFNGTCM------SEG-----QATTHVVIGMAGYRLSTDIPLTMPSWARVVDVSENGYTRLSVTSSRLEMTFISDVDARV 555          

BLAST of mRNA_A-nodosum_M_contig8530.1.1 vs. uniprot
Match: D8U202_VOLCA (Purple acid phosphatase n=1 Tax=Volvox carteri f. nagariensis TaxID=3068 RepID=D8U202_VOLCA)

HSP 1 Score: 342 bits (878), Expect = 3.620e-105
Identity = 234/652 (35.89%), Postives = 319/652 (48.93%), Query Frame = 1

Query:   76 LDPDITVSVSPTMLMK-SGEWVTIMYTGIESWRFPDAFVAAFSPGGVLDDPATITQVAPIKYQYLTAERPLLGDADAILDGKAGEEAGAGENQGELSTVQTLRFRLLNMRDPEGYSFGLFKGGIENPVLVAKTEE----------------PVMFARPYEVMHLHLALTAEANSMRVSWVTGAASQSPAVRY---------RQVTGGAGSWE------EVAGETLTYTREDMCGAPATTKGFHDPGLQHSAILTGLAPGMGYEYQAGDKEA-AEWSESTFFFAPPESLAKSAGDREAISSGEWGPEVVKMGVFGDMGTAEVDGTLDAGHSEEVPSLQTVAHLKEQLGVSLQLAAAEGGALGRDPRQEPPLSLVLHIGDLSYARGYDAQWDEYLDQIAPIASAVPWMVGVGNHERDSTAISPIRPELPYYTGTDSGGDCGVPTAFRFLMPGDAGKPLDDKPWYGFDHGPVHFTIMSTEHDFTPGSTQYLFIEEDLAGVDRTKTPWLVFAGHRPMYVNSGGPGASDCEGPATEGEHCATDQPVARLMRVSLEPLLLQYQVDLSVYGHHHSYQRTCKVADMVCKGVSTRSSEGGAEGYVAPVHVVLGMAGMGLSQNMVSPPPEWLEYATDREFGLCTLVADRSKLQLSFILNSDG 1932
            LDP I + +    L + SG+W  + ++G+   R+ D ++A  +P       A +++ AP K+++        GD   ++ G                   +LRFRL++ R     +F L + G + P  VA+++                  V    P E + +HLALT   + MRV W T  A  +P VR+         R   G AG  +        A +T  Y  ED+CG  AT+ G+ D G  H A+LTGL P   Y Y+ GD +    WS    F + PE           IS      E V +    DMG AEVDG+L+   SE +PSL T   + E+   S                   P SL+LHIGD+SYARGY  QWD ++ QI P+A+ +P+MV  GNHERD            ++   DSGG+CGV    RF MP     P  DK WY F +GP+ F + STEH   PGS QY FI + L GVDR +TPWLV AGHRP+YV S            T       DQPV+ L+R +LE L L++ VD+++ GHHHSYQRTC +     +GV   S++ G     APVHVVLG AG GLS N+V P P WLE      +G   +   RS+L +  + + DG
Sbjct:   89 LDPKIHIQLDRQFLERGSGDWFNVSWSGVTDPRYDD-WIALVAPSD-----ANLSETAPAKWKFAA------GDPKHVITGSG-----------------SLRFRLISYR--ADVAFALMRNGFDTPQEVARSQPIKRSLSSKPCSSAGAVTVRLLNPNEPLQVHLALTGSPSEMRVQWNTREAGSTPQVRWGPKSVKYDDRDGLGFAGGSDGPAYPSTAAADTSRYGIEDLCGGAATSAGWVDAGHHHVALLTGLRPATRYYYRVGDPDGDGGWSPEFSFLSSPE-----------ISP----DETVHILAVADMGQAEVDGSLEG--SEMIPSLNTTRRMIEEAAAS-------------------PYSLLLHIGDISYARGYSTQWDNFMHQIEPLAARMPYMVAPGNHERDWPGSG------DFFGVEDSGGECGVAYERRFPMP----YPGKDKQWYAFAYGPIFFILYSTEHPVGPGSEQYEFIVQALRGVDRRRTPWLVVAGHRPIYVAS------------TNANWPDGDQPVSELLRDALEDLFLEHAVDMTLQGHHHSYQRTCPLY----RGVCQPSNDDGTA--AAPVHVVLGHAGAGLSLNIVDPLPAWLENL-GLWWGYVRMKVSRSQLLVEVVGDDDG 644