mRNA_A-nodosum_M_contig1038.21.1 (mRNA) Ascophyllum nodosum dioecious

BLAST of mRNA_A-nodosum_M_contig1038.21.1 vs. uniprot
Match: A0A6H5K8T1_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K8T1_9PHAE)

HSP 1 Score: 546 bits (1407), Expect = 5.590e-175
Identity = 324/713 (45.44%), Postives = 419/713 (58.77%), Query Frame = 1

Query:  580 LGDVQHALELVSKFDSDGRYYSPSGKMLYLKDNPWKMPPEAVVEQGLEAIKIFLGDVRKARENGAQVRSLKHLKVVLVGSASAGKTSLMESIIKGTKSPTEGTLEEASTVGIDLRHHLLQGTKVEFYDCAGQVDYAGMHQTFLTRRALYLLLWDVTLFHGLDPRYLEKAVYRNIMRWLYTLHLRAPGATMILVANKCDGSLADFAESTERVKILVDRLLKEWQSRRSSNHRNGGDVTVVRLLDGISRTSCVDYG-----GIDELVERFSKESATSIQVPPAWDLALEVLDDLRAGGDPLQAARAHLCLKE---TKGTNKKVINTFRTKNQLLKLWEDIVRQVSEELQSAKDMAAVSNWENALDGALWINEFAGQILRIDRDEGIFLDVVWLSKVLSPILSHKLHAEDFPTI-----------DLKRQGYPSSSRCSPVGFCTTHLERCPDPCRTGIKGKIDVN*IYFPCSPQAWRDPSPGTAGLFVRPQRVARVTWRRRI*RYVGNHASGRGM*R*TAKSY*FFCRECTPGLSG------VKLKWECDAAQSPYGLVERLISSCNVIGEVEQDLCWRYGALFTSPEMIWGDDRQVRLYKFLISYDN-----RMLSLRIIGLFSDDRVLAALRYVASAMVVLSMEWPGVQWEGWLDCLNHPQARIYLTPPGETRIGGPLLDQGAYAHSMMCDCRREADGVLRLVRQRLGTVVDT 2628
            LG+ + AL LV +           G  LYL  NPW MPPE VVE GL A+K +L DVR+A++ GA V SL+ LKVVLVGSASAGKTSLM SII G  SPTEGT  +ASTVG++LR   LQ TKVEF+DCAGQVDYAGMHQTFLTRRALYLL+ D+T +HG+D   L+KA++ +IMRWLY L+LRAPG+T++LVANKCDGS+  FAE+ ER++  V  L+  W   R    R+ G VT + LL G+SR SC + G     G+  L+   S+++AT+I VPPAWDLALEV+  LRA  +P+ AAR  L L       G        F +K++L + W  +V  + +++ +A     +SNWE+AL+GALWI++FAG  LR+D  +GIFLDV+WLSK LSPILSHKL  + F               + R+ +        VG      E   +   + +  K+ V       +  A  D S  TA   V       V  R  +      H                  R    GL+       V LKWE D A  PYGL+ERLI+SC+V+GEVE   CWRYGA+F S +M + + R VR YK  +S  +     R+ + ++ G    +RV  A+RYVASAM+ LS EWPGV W GWLDC  HP   +Y+    E R+G PLL   A   +  CDC  E  GVLRL  + LG V+DT
Sbjct:    9 LGETEDALRLVERL----------GDSLYLHGNPWIMPPEGVVEAGLTAVKRYLVDVREAKKAGADVTSLQLLKVVLVGSASAGKTSLMHSIIAGRGSPTEGTPAQASTVGMELRPCQLQDTKVEFFDCAGQVDYAGMHQTFLTRRALYLLVVDITKYHGVDD--LDKAIHDDIMRWLYYLYLRAPGSTVVLVANKCDGSIDKFAETAERIQRRVRELVAVWHEARGLRGRSKGHVTDLTLLPGMSRVSCQEKGSPEASGLPALIALISRQAATAIHVPPAWDLALEVIGALRASCNPIAAARDKLGLPNPLPADGVADVHAFAFISKDELSRKWRSVVDNLRDDVPAAM----ISNWESALEGALWISDFAGHTLRVDGGDGIFLDVLWLSKCLSPILSHKLKNQPFENRWLWMRDDLAGDGILRREFALHIWRESVGEVVMESEEVAEALFS-VLIKLGVALPMGRRTLLAHSDRSTPTAPSDVSGSNDMLVIMR--LSETCNEHQ-----------------RTLFDGLASKMPRDRVTLKWEFDKAGPPYGLIERLIASCHVLGEVETGACWRYGAVFQSHDMRFVEGRNVRFYKIALSMTSYNESERVFTAKVFGPLDSERVWVAIRYVASAMINLSKEWPGVLWTGWLDCEKHPSQHLYMASSVEARVGDPLLPAAA-PDARRCDCLLEPGGVLRLAVEELGPVIDT 684          

BLAST of mRNA_A-nodosum_M_contig1038.21.1 vs. uniprot
Match: A0A6H5JW02_9PHAE (Roc domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JW02_9PHAE)

HSP 1 Score: 509 bits (1310), Expect = 7.250e-159
Identity = 313/715 (43.78%), Postives = 420/715 (58.74%), Query Frame = 1

Query:  580 LGDVQHALELVSKFDSDGRYYSPSGKMLYLKDNPWKMPPEAVVEQGLEAIKIFLGDVRKARENGAQVRSLKHLKVVLVGSASAGKTSLMESIIKGTKSPTEGTLEEASTVGIDLRHHLLQGTKVEFYDCAGQVDYAGMHQTFLTRRALYLLLWDVTLFHGLDPRYLEKAVYRNIMRWLYTLHLRAPGATMILVANKCDGSLADFAESTERVKILVDRLLKEWQSRRS-SNHRNGGDVTVVRLLDGISRTSCVDYGGIDELVERFSKESATSIQVPPAWDLALEVLDDLRAGGDPLQAARAHLCL--KETKGTNKKVINTFRTKNQLLKLWEDIVRQVSEELQSAKDMAAVSNWENALDGALWINEFAGQILRIDRDEGIFLDVVWLSKVLSPILSHKLHAEDFPTIDLKRQGYPSSSRCSPVGFCTTHLER------CPDPCRTGIKGKIDVN*IYFPCSPQAWRDPSPGTAGLFVRPQRVARVTWR--RRI*RYVGNHASGRGM*R*TAKSY*FFCRECTPGLSGVKLKWECDAAQSPYGLVERLISSCNVIGEVEQDLCWRYGALFTSPEMIWGDDRQVRLYKFLISYDN------RMLSLRIIGLFSDDRVLAALRYVASAMVVLSMEWPGVQWEGWLDCLNHPQARIYLTPPGETRIGGPLL-DQGAYAHSMMCDCRREADGVLRLVRQRLGTVVDTRLF-VPEVRGEKRS 2667
            +G+ +HAL  +++          S  ++ L  NPW MPPE VVE+GL A++ +L DVRKA E GA+V++LK LKVVLVGS+ AGKTSL+ SI++G   PT GT  E STVGI+L  H LQGT VEFYDCAGQVDY GMHQ FLTRRALYLL+WDV   HG     L++ ++++IMRWLY LHLRAPG+T+ILVANKCDGS+ DF  +   V+  V  L KEW+  R  S+ R   D+TV   L+  S  SC+D  G+ E+++R + +SA S++VPPAW LAL  LD LR   DP QA R  L L  +   G +++   +F T+N L++ W+ +V+ V  EL+S  +  AVS+ + A++GALWI+EFAGQ+LR+   +G+FLDVVWLS  L PIL HKL  + FP      +     +    + F   HL         P+     +   I    +  P  P +   P  G A    RP  ++R       R+    G     + +     + +    RE T       L W  D+A  PYGLVER+I+SC+ +G VE  LCWRYGA+F S  M   D    RLY F++ YD+      R+LS+R+ G   D+RV AALR+VAS++V +S +WPGV WEGW  C    + R Y     +  IG  LL D    A    CDC      VLRLV +RLG VVDT+     +V G +R+
Sbjct:  161 VGEAEHALRWLTR---------RSECLIVLGGNPWIMPPEGVVEKGLPAVESYLLDVRKAEEAGAEVKTLKLLKVVLVGSSQAGKTSLVNSIVEGRGLPTVGTPAEVSTVGIELLRHNLQGTAVEFYDCAGQVDYYGMHQNFLTRRALYLLVWDVLRCHGKTDDDLDEVIFQDIMRWLYVLHLRAPGSTVILVANKCDGSIEDFTGTAAVVEERVAVLRKEWRKARGFSSTRMDMDMTV---LERSSLVSCLDGVGLQEMIDRIAAQSAASMRVPPAWGLALTFLDALRDSRDPQQACRDCLSLGSRPKVGADREPGRSFFTENSLVEQWKGVVQNVEGELRSDAEKMAVSDPDGAIEGALWISEFAGQVLRVANGDGVFLDVVWLSTALKPILDHKLVHKVFPQHFAGMRNELVHNGILRIAFAE-HLWSDVMEITVPEEVLDALCRVIVNLGVALPLEPASLL-PGSGRALATGRPN-ISRQDMLVIMRLPETCGAEHELQELISDQMRRH--SDREVT-------LMWRFDSAGPPYGLVERVIASCHAVGVVEMGLCWRYGAVFQSRAMATSDGSD-RLYTFVVRYDSAVGNERRILSMRMFGPLQDNRVWAALRWVASSVVNISKDWPGVLWEGWPQCARRHRNRTYFATSDKASIGDFLLADAATGATRRGCDCLAVEGTVLRLVLERLGKVVDTQAPPASDVEGVRRT 850          

BLAST of mRNA_A-nodosum_M_contig1038.21.1 vs. uniprot
Match: D7FVX5_ECTSI (LRR-GTPase of the ROCO family n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FVX5_ECTSI)

HSP 1 Score: 490 bits (1261), Expect = 6.810e-150
Identity = 303/733 (41.34%), Postives = 395/733 (53.89%), Query Frame = 1

Query:  574 SDLGDVQHALELVSKFDSDGRYYSPSGKMLYLKDNPWKMPPEAVVEQGLEAIKIFLGDVRKARENGAQVRSLKHLKVVLVGSASAGKTSLMESIIKGTKSPTEGTLEEASTVGIDLR-HHLLQGTKVEFYDCAGQVDYAGMHQTFLTRRALYLLLWDVTLFHGLDPRYLEKAVYRNIMRWLYTLHLRAPGATMILVANKCDGSLADFAESTERVKILVDRLLKEWQSRRSSNHRNGGDVTVVRLLDGISRTSCVDYGGIDELVERFSKESATSIQVPPAWDLALEVLDDLRAGGDPLQAARAHLCLKETKGTNKKVINTFRTKNQLLKLWEDIVRQVSEELQSAKDMAAVSNWENALDGALWINEFAGQILRIDRDEGIFLDVVWLSKVLSPILSHKLHAEDFPTI------------------------DLKRQGYPSS----SRCS---------PVGFCTTHLERCPDPCRT-GIKGKIDVN*IYFPCSPQAWRDPSPGTAGLFVRPQRVARVTWRRRI*RYVGNHASGRGM*R*TAKSY*FFCRECTPGLSGVKLKWECDAAQSPYGLVERLISSCNVIGEVEQDLCWRYGALFTSPEMIWGDDRQVR-LYKFLISYDN------RMLSLRIIGLFSDDRVLAALRYVASAMVVLSMEWPGVQWEGWLDCLNHPQARIYLTPPGETRIGGPLL-DQGAYAHSMMCDCRREADGVLRLVRQRLGTVVDTR 2631
            S LG  + AL+ V++          SG+ L L+DNPW MPPE +V +GL A++ +L DVR+A E GA+V++L  LKVVL+GS  AGKTSL+ SI+    S T GT EE STVGI+LR HH   G  VEFYDCAGQVDY GMHQTFLTRRALYLL+WDV+  HG +   L+K V  +IM+WLY LHLR PG+ ++LVANKCDGSL +F  +   V+    R L+EWQ  R S       VT + +L   S  SC D GG+ E++ R   + ATSI VPP+W LAL  LD LR   DP  A R  L L           ++F T++ L + W D V+ V+ EL S  +  AVS+   A++GALWI+EFAG ILR+   + IFLDVVWLS  L PILSHKL +  FP                          DL+ +  PS     + C          P+G    H     DP    G  G+ D+  +     P    +   G     VR              R+ GN                            V LKW  D A + +GLVER+I+SC+ +G V   LCWRYGA+F S      DD  +  LY F+I YD       R+L++ + G  ++DR+ AALR+VAS++V  S  WPGV+WEG   C  H + R+Y   P E RIG P+L D        +CDC       +RLV +RLGT++DT+
Sbjct:  316 SALGQAEQALQFVARLGQ-----LASGQNLRLQDNPWVMPPEGIVNEGLPAVEKYLRDVREAEEAGAEVKTLNVLKVVLIGSPGAGKTSLLNSIVNERGSRTVGTSEEVSTVGIELRRHHQRNGRIVEFYDCAGQVDYYGMHQTFLTRRALYLLVWDVSRCHGKESNDLDKVVSEDIMKWLYALHLRVPGSAVLLVANKCDGSLQEFVGTAGAVESSARRQLQEWQGNRKSP-----GVTKLTILQQPSLVSCDDGGGLSEVIARVDAQGATSIVVPPSWGLALAFLDALRDSRDPEPATRRFLHLDFDAEVGAAQGSSFMTESTLFQRWNDTVKSVAGELTSPAERMAVSDHHGAMEGALWISEFAGHILRVAHSDVIFLDVVWLSAALKPILSHKLESHFFPRSELTAMKDELVDNGVLRLKFAEYLWDLEMEAPPSEEVMDALCGVLHSLGVALPLGREERHTASAIDPAAAHGQIGRQDM--LVIMRLPDTCEENIQGELQELVRDLSE----------RHGGNE---------------------------VTLKWRFDGAGASHGLVERVIASCHEVGSVRSGLCWRYGAVFQSYAR--ADDGGLNWLYSFVIRYDEEVGSGERVLAVTMFGPTAEDRLWAALRWVASSVVNTSTGWPGVRWEGGPACAIHARGRMYFKTPIEARIGDPILTDPDPRTAPNVCDCYTTEGNAMRLVLERLGTIIDTQ 997          

BLAST of mRNA_A-nodosum_M_contig1038.21.1 vs. uniprot
Match: A0A6H5L650_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L650_9PHAE)

HSP 1 Score: 195 bits (495), Expect = 7.440e-48
Identity = 187/689 (27.14%), Postives = 294/689 (42.67%), Query Frame = 1

Query:  649 SGKMLYLKDNPWKMPPEAVVEQGLEAIKIFLGDVRKARENGAQVRSLKHLKVVLVGSASAGKTSLMESIIKGTKSPTEGTLEEASTVGIDLRHHLLQGTKVEFYDCAGQVDYAGMHQTFLTRRALYLLLWDVTLFHGL-----------DPRYLEKAVYRNIMRWLYTLHLRAPGATMILVANKCDGSLADFAESTERVKILVDRLLKEWQSRRSSNHRNGGDVTVVRLLDGISRTSCVDYGGIDE---------------------------------LVERFSKESATSIQV--PPAWDLALEVLDDLRAGGDP-----LQAARAHLCLKETKGTNKKVINTFRTKNQLLKLWEDIVRQVSEELQSAKDMAAVSNWENALDGALWINEFAGQILRIDRDEGIFLDVVWLSKVLSPILSHKLHAEDFPTIDLKRQG-----------YPSSSRCSPVGFCTTHLERC--PDPCRTGIKGKIDVN*IYFPCSPQAWRDPSPGTAGLF-VRPQRVARVTWRRRI*RYVGNHASGRGM*R*TAKSY*FFCRECTPGLSGVKLKWECDAAQSPYGLVERLISSCNVIGEVEQDLCWRYGALFTSPEMIWGDDRQVRLYKFLISYDNRMLSLRIIGLFSDDRVLAALRYVASAMVVLSMEWPGVQWEGWLDCLNHPQARIYL---TPPGETRI 2511
            +G+ L L  NPW  PPE++V +GL++++ +  D+     +  +++  + +KVVLVG   AGKTSL +S+ K  K+   G  +E STV  D+    L+G+ V  YDCAGQV Y G+ Q FLT R++ +L+ +   F              D R LE      +  WL ++  R PG  +ILVA KCD    +  E+ +R++      L  W        RNG D   VRL   +S TSC   G  +                                  LV +  +     +Q+  P +WD+AL  LD L  G  P     L++  +     ET G    V     T  +L   W+  V ++       +    V+N ENAL+GAL I EF G +  +  ++ +FLDVVWL+++L P+L+HK        ++L   G             S  R    G     L     PD     +   +    + FP       DP+ G   L  ++P R   V                        +    FC   TP     +  W+      P G VE++++ C  +G V+    WRYG L      +  + R   +     S DN +++ ++ G  S      A+ YV SA+ ++ +++PG++W+G L C  H    +++   +PP   R+
Sbjct:  129 AGRHLSLNSNPWAEPPESIVAKGLKSVRGYFEDLFA---DHCRIKR-RGVKVVLVGQEGAGKTSLRQSM-KANKATPTGEWKEESTVFADVEPMKLEGSSVRVYDCAGQVAYTGLLQMFLTPRSVCVLVCNAGEFRQQLGSDNIDQVEEDCRKLEGL---RVCDWLRSISCRVPGNDVILVATKCDLVNGNVEETGQRIEHACRTWLSSWV-------RNGMDS--VRLERHVSLTSCFPIGASEHGESRRGNHALKQGWACDWRDVEGDMSSPSLLYRLVNKPDQGGLRGVQMVLPRSWDVALTFLDSLEYGRHPVEMVMLKSPDSDSGATETAGGTTTVYQGI-TVEELSARWQGTVDELG------RRGITVTNAENALEGALSIREFDGSL--VCHEKFVFLDVVWLARILKPLLNHKDQEMFDGRVNLGDTGDTRITLDDPSDIASWDRLKNEGVLEPRLADAIWPDGLSEYVLPTLASLGLIFPLE----NDPAEGLVVLLRLKPHRPESV-----------------------GEVIDTFCSGLTPAF---RASWKVFLGVPP-GAVEKVLTRCCALGGVQT--FWRYGVLVHGGLGVQDERRTFAVVVEYSSTDNELVA-QVFGDISRPGPWMAMSYVMSAVSLMLVDFPGLRWKGSLKCPQHGDGMLFVNKVSPPHAYRL 757          

BLAST of mRNA_A-nodosum_M_contig1038.21.1 vs. uniprot
Match: D7FL98_ECTSI (LRR-GTPase of the ROCO family n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FL98_ECTSI)

HSP 1 Score: 198 bits (504), Expect = 7.700e-48
Identity = 187/690 (27.10%), Postives = 296/690 (42.90%), Query Frame = 1

Query:  673 DNPWKMPPEAVVEQGLEAIKIFLGDVRKARENGAQVRSLKHLKVVLVGSASAGKTSLMESIIKGTKSPTEGTLEEASTVGIDLRHHLLQGTKVEFYDCAGQVDYAGMHQTFLTRRALYLLLWDVTLFHGLD---------PRYLEKAVYRNIMRWLYTLHLRAPGATMILVANKCDGSLADFAESTERVKILVDRLLKEWQSRRSSNHRNGGDVTVVRLLDGISRTSCV-----DYGGI--------DE-------------------LVERFSKES-----ATSIQVPPAWDLALEVLDDLRAGGDPLQAARAH-LCLKETKGTNKKVINTFR--------TKNQLLKLWEDIVRQVSEELQSAKDMAAVSNWENALDGALWINEFAGQILRIDRDEGIFLDVVWLSKVLSPILSHKLHAEDF--------PTIDLKRQGYPSS-SRCSPVGFCTTHLERC--PDPCRTGIKGKIDVN*IYFPCSPQAWRDPSPGTAGLF----VRPQRVARVTWRRRI*RYVGNHASGRGM*R*TAKSY*FFCRECTPGLSGVKLKWECDAAQSPYGLVERLISSCNVIGEVEQDLCWRYGALFTSPEMIWGDDRQVRLYKFLISY--DNRMLSLRIIGLFSDDRVLAALRYVASAMVVLSMEWPGVQWEGWLDCLNHPQARIY---LTPPGETRIGG 2517
            +NPW+ PPEAVV +G+ A++ +  D+      G      K +KVVLVG   AGKTSL +S+  G  +PT G+  E STV ID+    + G  +  YDCAGQV Y G+ Q FL+ RA+ LL+ D+  F   D          + L K     +  WL +L  R P + +++VA KCD +     ++  R++  +   L+ W         +G  +  V + DG+S TSC      + GG         DE                   L+ R    S       S+ +P +W++ALEVLD L +G +P+++A+ + L     KG  + V   ++        T+  L   W ++V  +        D  +V   ++AL+GAL I E  G ++R   +  +FLDV WL+++L P+L+H+   +            I LK   + +S +R    G     L R   PD     +   +D   + +P       DP+ G   L      RP+ V                           K    F R+ T  LS   +KW+      P G +E++++ C  +G +     WR+G L        G     R +  ++ Y  D   + +++ G  S     AAL +  S    +  E+PG++W   L C  H Q        T PG+  + G
Sbjct:  247 ENPWQEPPEAVVSKGISAVQEYFTDLFA---EGIAPVGRKMIKVVLVGQEGAGKTSLRQSLRTGRPTPTSGS--EESTVQIDVEQMDVNGVTLRVYDCAGQVAYTGLLQMFLSPRAVSLLVCDLGAFGQRDGTLIHSDQLKKDLSKLQELRVCDWLRSLSFRIPDSDVVVVATKCDLAAGMGTDAAGRIEGAIGEWLESW---------SGAGMNAVHVEDGVSLTSCFPPTPNEDGGAALGKRKSPDEDSTWACDWREGVSVESQPSLLHRVMYNSKGNVRGASLVLPRSWNIALEVLDALGSGRNPVESAQQNVLASGGPKGATEVVPAVYQRNQGVGGLTRADLSTKWNEVVAALE------GDGVSVVKPDHALEGALLIREHEGSLVR--HETYVFLDVTWLAQILKPLLNHRDDEDPLRGDVSLGDTGITLKEDKHIASWNRLKGHGVLEPSLARVLWPDGLSNYVLPALDSLGLTYPLDD----DPAEGLVVLLRLDEKRPEDVG--------------------------KELDDFRRDHTAVLS---VKWKVFLGVPP-GAIEKMLTRCCRLGTLRT--FWRFGVLVQGS---LGGGIAARTFALVVEYSHDKCEVDMKVYGSISTAAPWAALSFGISVFQAMCSEFPGLRWRAALKCPQHEQDMQISKAATRPGDKSLFG 875          

BLAST of mRNA_A-nodosum_M_contig1038.21.1 vs. uniprot
Match: D8LJS7_ECTSI (LRR-GTPase of the ROCO family n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LJS7_ECTSI)

HSP 1 Score: 198 bits (504), Expect = 8.060e-48
Identity = 189/705 (26.81%), Postives = 299/705 (42.41%), Query Frame = 1

Query:  601 LELVSKFDSDGRYYSPSGKMLYLKDNPWKMPPEAVVEQGLEAIKIFLGDVRKARENGAQVRSLKHLKVVLVGSASAGKTSLMESIIKGTKSPTEGTLEEASTVGIDLRHHLLQGTKVEFYDCAGQVDYAGMHQTFLTRRALYLLLWDVTLFHGL-----------DPRYLEKAVYRNIMRWLYTLHLRAPGATMILVANKCDGSLADFAESTERVKILVDRLLKEWQSRRSSNHRNGGDVTVVRLLDGISRTSCV--------------------------DYGG-------IDELVERFSKESATSIQV--PPAWDLALEVLDDLRAGGDPLQAA--RAHLCLKETKGTNKKVINTFRTKNQLLKLWEDIVRQVSEELQSAKDMAAVSNWENALDGALWINEFAGQILRIDRDEGIFLDVVWLSKVLSPILSHKLHAEDFPTIDLKRQG-----------YPSSSRCSPVGFCTTHLERC--PDPCRTGIKGKIDVN*IYFPCSPQAWRDPSPGTAGLF-VRPQRVARVTWRRRI*RYVGNHASGRGM*R*TAKSY*FFCRECTPGLSGVKLKWECDAAQSPYGLVERLISSCNVIGEVEQDLCWRYGALFTSPEMIWGDDRQVRLYKFLISYDNRMLSLRIIGLFSDDRVLAALRYVASAMVVLSMEWPGVQWEGWLDCLNHPQARIYLTPPGETRIGGPLLD 2529
            L++    ++ G   +  G  L L +NPW  PPE++V +G ++I+ +  D+        +++  + +KVVLVG   AGKTSL +S+ K  K+   G  +E STV  D+    L+G+ V  YDCAGQV Y G+ Q FLT R++ +L+ +   F              D R LE      +  WL ++  R P   +ILVA KCD +  +  E   R++    R L  W  R         ++  VRL  G+  TSC                           D GG       I  LV +  K     +Q+  P +WD+AL  LD L  G DP++    ++    +    T K  +    T  +L   W++ V  +       +    V+N ENAL+GAL I EF G +  +  ++ +FLDVVWL+++L P+L+HK        ++L   G             S  R    G     L     P+     +   +    + FP       DP+ G   L  ++P R   V                        K    FC   TP     +  W+      P G +E++++ C  +G V+    WRYG L      +  + R   +     S DN +++ +I G  S      A  YV SA+ ++ +++PG+ W+G+L+C  H    +++     TR G   L+
Sbjct:  284 LDIFDSVEAQGLNDTGIGPSLSLSENPWAEPPESIVAKGPKSIRGYFEDLYAEH---CRIKR-RGVKVVLVGQEGAGKTSLRQSM-KANKATLTGEWKENSTVLADVESMELEGSSVRVYDCAGQVAYTGLLQMFLTPRSVCVLVCNAGEFGEQLGSDNIDQVEEDCRKLEAL---RVCNWLRSISRRVPDTEVILVATKCDLAGGNAWEIGRRMEKACRRWLSSWACR---------NMLSVRLEPGVCLTSCFPVGAGEHGESSSENFLSKSCWACDWRDVGGGEPSPSLIYRLVNKPDKGGLRGVQMVLPRSWDIALTFLDSLEHGRDPVEMVILKSPDSDRGATETAKTTVYQGITVEELSAKWQETVDGLG------RRGITVTNAENALEGALSIREFDGSL--VPHEKFVFLDVVWLARILKPLLNHKDQEMFDGRVNLGDTGDTRITLDDSADIASWDRLKKEGVLEPRLAYAIWPNGLSEYVLPTLASLGLIFPLE----NDPAEGLVVLLRLKPHRPESV-----------------------GKVIDTFCSGLTPAF---RASWKIFLGVPP-GAIEKVLTRCCGLGGVQT--FWRYGVLVHGGLGVQDERRTFAVVVEYSSTDNELIA-QIFGDVSTPGPWGATSYVMSAVSLMLVDFPGLGWKGYLECPQHGDGMLFVNKA--TRDGDKFLE 927          

BLAST of mRNA_A-nodosum_M_contig1038.21.1 vs. uniprot
Match: D7FZA1_ECTSI (LRR-GTPase of the ROCO family, incomplete sequence n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FZA1_ECTSI)

HSP 1 Score: 194 bits (494), Expect = 6.500e-47
Identity = 197/681 (28.93%), Postives = 287/681 (42.14%), Query Frame = 1

Query:  649 SGKMLYLKDNPWKMPPEAVVEQGLEAIKIFLGDVRKARENGAQVRSLKH--LKVVLVGSASAGKTSLMESIIKGTKSPTEGTLEEASTVGIDLRHHLLQGTKVEFYDCAGQVDYAGMHQTFLTRRALYLLLWDVTLFH---GLD-----PRYLEKAVYRNIMRWLYTLHLRAPGATMILVANKCDGSLADFAESTERVKILVDRLLKEWQSRRSSNHRNGGDVTVVRLLDGISRTSCVDYG---------GID------------------------ELVERFSKESATSIQV--PPAWDLALEVLDDLRAGGDPLQAARAHLCLKETKGTNKKVINTFRTKNQLLKL------WEDIVRQVSEELQSAKDMAAVSNWENALDGALWINEFAGQILRIDRDEGIFLDVVWLSKVLSPILSHKLHAEDFPTIDLKRQG-----------YPSSSRCSPVGFCTTHLERC--PDPCRTGIKGKIDVN*IYFPCSPQAWRDPSPGTAGLF-VRPQRVARVTWRRRI*RYVGNHASGRGM*R*TAKSY*FFCRECTPGLSGVKLKWECDAAQSPYGLVERLISSCNVIGEVEQDLCWRYGALFTSPEMIWGDDRQVRLYKFLISYD--NRMLSLRIIGLFSDDRVLAALRYVASAMVVLSMEWPGVQWEGWLDCLNHPQARIYLT 2490
            SG  L L  NPW  PPE++V +G ++I+ +  D+       A+   +K   +KVVLVG   AGKTSL +S+ K  K+   G  +E STV  D+    L+G+ V  YDCAGQV Y G+ Q FLT R++ +L+ +   F    G D          K     +  WL ++  R PG  +ILVA KCD    +  E+  R++      L  W        RNG D   VRL   +S TSC   G         G D                         LV +  +     +Q+  P +WD+AL VL+ L  G DP++         E + T   V  T     Q +K+      W + V ++       K    V+N ENAL+GAL I EF G ++R   ++ +FLDVVWL+++L P+L+HK        ++L   G             S  R    G     L     PD     +   +    + FP       DP  G   L  + P R A V                        ++   FC + TP  S     WE      P G +E++++ C  +G+V+    WRYG L        GD     ++  +  Y   N  L+ +I G  S      AL YV SA+ ++ M++PG++W G L C  H     + T
Sbjct:   80 SGVNLALLGNPWAEPPESIVAKGPKSIRGYFEDLY------AEPCWIKRSSVKVVLVGQEGAGKTSLRQSM-KANKATPTGEWKEESTVFADVEPMELEGSSVRVYDCAGQVAYTGLLQMFLTPRSVCVLVCNAGEFGQQLGSDNVDQVEEDCRKLQALRVCDWLRSISRRVPGNDVILVATKCDLVSGNAEETGRRLEHACRMWLSTWV-------RNGMDP--VRLEPHVSLTSCFPIGDGEHGESNTGSDASKQGWACDWRHVEGDKSSPSLLYRLVNKPDEGGLRGVQMVLPRSWDIALTVLEALEHGRDPVEMVLLKSPDSERRPTETGVGKT--NVYQAIKVEELGTNWRETVDELG------KRGIVVTNAENALEGALSIREFDGSLVR--HEKFVFLDVVWLARILKPLLNHKDQRTFDGRVNLGDTGDARITLDDSSDIASWGRLKNEGVLEPRLAYAMWPDGLSEYVLPTLASLGLTFPLE----GDPDNGLVVLLRLEPNRPASV-----------------------GEAIDTFCSKYTPAFSA---SWEIFLGVPP-GAIEKVLTRCCGLGDVQT--FWRYGVLVHGG---LGDLDGRGIFAVVTEYSSTNNELTAQIFGDISTSAPWVALSYVTSAVSLMLMDFPGLRWNGSLKCPQHGYEMPFAT 698          

BLAST of mRNA_A-nodosum_M_contig1038.21.1 vs. uniprot
Match: D7FUC9_ECTSI (LRR-GTPase of the ROCO family n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FUC9_ECTSI)

HSP 1 Score: 184 bits (468), Expect = 1.230e-43
Identity = 198/735 (26.94%), Postives = 303/735 (41.22%), Query Frame = 1

Query:  649 SGKMLYLKDNPWKMPPEAVVEQGLEAIKIFLGDVRKARENGAQVRSLKHLKVVLVGSASAGKTSLMESIIKGTKSPTEGTLEEASTVGIDLRHHLLQGTKVEFYDCAGQVDYAGMHQTFLTRRALYLLLWDVTLFHGL-----------DPRYLEKAVYRNIMRWLYTLHLRAPGATMILVANKCDGSLADFAESTERVKILVDRLLKEWQSRRSSNHRNGGDVTVVRLLDGISRTSCVDYGGIDELVERFSKESA------------------------------------TSIQVPPAWDLALEVLDDLRAGGDPLQAARAHLC---LKETKGTNKKVINTFR--TKNQLLKLWEDIVRQVSEELQSAKDMAAVSNWENALDGALWINEFAGQILRIDRDEGIFLDVVWLSKVLSPILSHKLHAEDFPTIDLKRQG-----------YPSSSRCSPVGFCTTHLERC--PDPCRTGIKGKIDVN*IYFPCSPQAWRDPSPGTAGLF-VRPQRVARVTWRRRI*RYVGNHASGRGM*R*TAKSY*FFCRECTPGLSGVKLKWECDAAQSPYGLVERLISSCNVIGEVEQDLCWRYGALFTSPEMIWGDDRQVRLYKFLISY---DNRMLSLRIIGLFSDDRVLAALRYVASAMVVLSMEWPGVQWEGWLDCLNHPQARIY-----------LTPPGETRIGGPLLDQGAYAHSM--MCDCRREADGVLRLVRQR 2607
            +G +L L DNPW  PP ++V +G  +I+ +  D+     +  Q  S+K   ++LVG   AGKTSL +S+      PT G  +E STV  D+    L+G+ V  YDCAGQV Y G+ Q FLT R++ +L+ +   F              D R LE+     +  WL ++  R P   +ILVA KCD    +  E   R++      L  W             +  VRL  G+  TSC     + E  E+ +  +A                                    T + +P +WD+AL VL+ L  G DP++    HL     ++   T  +    ++  T   L   W++ V      +  A+    V+N +NAL+GAL I EF G ++R   +  +FLDVVWL+++L P+L+HK        ++L   G             S  R    G     L R   P+     +   +    + FP       DP+ G   L  + P R  RV                        K    FC + TP  S     W+      P G +E++++ C  +G V+    WR G L        GD+    ++  ++ Y   DN  L+ +I G  S      AL YV SA+ ++ +++PG++  G L C  H  A +            L P G  +        GA A  +  M D R + D + R V+QR
Sbjct:  193 TGGILGLDDNPWAEPPASIVSKGPTSIRGYFEDLY-VESSRVQRNSVK---IILVGQEGAGKTSLRQSMKANAAIPT-GEWKEESTVFADVEPMQLEGSFVRVYDCAGQVAYTGLLQMFLTPRSVCVLVCNAEAFGQQRGDDTGGQVKEDCRKLEEL---RVCDWLRSVSRRVPNNDVILVATKCDLVGGNAEEFGRRIEQATRTWLASWVH---------DGMQPVRLEPGVCLTSCC-ASAVGENGEKSTGHNASERGWACDWRDDRDDMPPPGLVHRLVNKPDGSGLRGTQMVLPRSWDIALTVLEALERGRDPVEVVVEHLAEPDREDATRTAARQTGVYQGITVEALRVKWQEAV------VGLARRGVNVTNAKNALEGALSIREFDGSLVR--HEMFVFLDVVWLARILKPLLNHKDEETFDGLVNLGDTGDTRVTLEDPLDIASWGRLKNQGVLEPRLARTMWPNGLSKYVLPTLASLGLTFPLK----NDPAGGLVVLLRLNPDRPERV-----------------------GKVIDTFCLDQTPAFS---TSWKIFLGV-PAGAIEKVLTRCCSLGGVQT--FWRSGVLVHGG---LGDEDGHEIFAVVLEYSSSDNE-LTAQIFGDISTPAPWVALSYVISAVRLMLLDFPGLRSRGSLKCPQHGDAMLLVNKMTQAGVKLLEPSGCPQCSPDTRGLGAAAIDLVSMVDIRLDRDVIFRDVKQR 864          

BLAST of mRNA_A-nodosum_M_contig1038.21.1 vs. uniprot
Match: A0A6H5KSZ8_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KSZ8_9PHAE)

HSP 1 Score: 184 bits (466), Expect = 1.360e-43
Identity = 200/711 (28.13%), Postives = 299/711 (42.05%), Query Frame = 1

Query:  661 LYLKDNPWKMPPEAVVEQGLEAIKIFLGDVRKARENGAQVRSLKHLKVVLVGSASAGKTSLMESIIKGTKSPTEGTLEEASTVGIDLRHHLLQGTKVEFYDCAGQVDYAGMHQTFLTRRALYLLLWDVTLFH-----------GLDPRYLEKAVYRNIMRWLYTLHLRAPGATMILVANKCDGSLADFAESTERVKILVDRLLKEWQSRRSSNHRNGGDVTVVRLLDGISRTSCVDYG---------GIDELVERFSKE--------------------------SATSIQVPPAWDLALEVLDDLRAGGDPLQAARAHLCLKETKGTNKKVINTFRTKNQLLK--LWEDIVRQVSEELQSA-KDMAAVSNWENALDGALWINEFAGQILRIDRDEGIFLDVVWLSKVLSPILSHKLHAEDFPTIDLKRQG-----------YPSSSRCSPVGFCTTHLERC--PDPCRTGIKGKIDVN*IYFPCSPQAWRDPSPGTAGLF-VRPQRVARVTWRRRI*RYVGNHASGRGM*R*TAKSY*FFCRECTPGLSGVKLKWECDAAQSPYGLVERLISSCNVIGEVEQDLCWRYGALFTSPEMIWGDDRQVR-----LYKFLISY---DNRMLSLRIIGLFSDDRVLAALRYVASAMVVLSMEWPGVQWEGWLDCLNHPQARIYLTPPGETRIGGPLLDQGAYAHSM--MCDCRRE 2574
            L L  NPW  PPE +V +G +A++ +  D+    E     RS+  +KVVLVG   AGKTSL +S+     +PT G  +E STV  D+    L+G+ V  YDCAGQV Y G+ Q FLT RA+ +L+ +   F            G D R LE+     +  WL ++  R P   +ILVA KCD +  +  +   R++    R L  W        RNG  +  VRL   +S TSC + G         G  EL   ++ +                            T + +P +WD+AL VL+ L  G DP++     +  K           T R +  + +    ED+  +  E +    +    V+N +NAL+GAL I EF G ++R ++   IFLDVVWL+K+L P+L+HK        + L   G             S  R    G     L     P+     +   +    + FP       DP+ G   L  ++P R   V            HA               FC + TP LS     W+      P G +E++++ C  +G V     WRYG L      + G   +V      ++  ++ Y   DN  L+ +I G  S       L YV SA+ ++ +++PG++W G L C  H    +            P+   GA A  +  M D RR+
Sbjct:  153 LKLGTNPWVEPPEPIVVKGPKAVRGYFEDLYA--EPCRIKRSI--VKVVLVGQEGAGKTSLRQSMKANEATPT-GEWKEESTVFADVEAMELEGSSVRVYDCAGQVAYTGLLQMFLTPRAVCVLVCNAGKFGQQTRSESNDQVGEDCRKLEEL---RVCDWLRSISRRVPNNDVILVATKCDLAGGNAGKIGARMEHACRRWLSSWV-------RNG--LQPVRLEPCVSLTSCFETGVGEHGESSTGSHELKGGWACDWRDMADGNPSPSLLHRLVNKPDGRGLRGTQMVLPRSWDIALTVLEALEHGRDPVEV----VLRKSPDPDRGDATETARGETAMYQGITVEDLSIKWQETVDGLERSGVTVTNAKNALEGALLIREFDGSLIRYEKF--IFLDVVWLAKILKPLLNHKDQETFDGLVSLGDTGDARITLDDPSDIASWGRLKSQGVLEPRLAYAIWPNGLSEYVLPALAFLGLTFPLED----DPAKGLVVLLRLKPDRPESV-----------GHAIDT------------FCLDHTPALSA---SWKIFLGVPP-GAIEKVLTRCCGLGGVRT--FWRYGVL------VHGGLGEVHVGRRGIFAVVLEYSSTDNE-LAAQIFGDISTPAPWVVLSYVMSAVSLMLLDFPGLRWRGSLTCPQHGDTMLLANK------ASPVAGLGAAAIDLVRMVDIRRD 794          

BLAST of mRNA_A-nodosum_M_contig1038.21.1 vs. uniprot
Match: D7G431_ECTSI (LRR-GTPase of the ROCO family, incomplete sequence n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G431_ECTSI)

HSP 1 Score: 179 bits (454), Expect = 5.350e-42
Identity = 192/683 (28.11%), Postives = 288/683 (42.17%), Query Frame = 1

Query:  661 LYLKDNPWKMPPEAVVEQGLEAIKIFLGDVRKARENGAQVRSLKHLKVVLVGSASAGKTSLMESIIKGTKSPTEGTLEEASTVGIDLRHHLLQGTKVEFYDCAGQVDYAGMHQTFLTRRALYLLLWDVTLFHGL-----------DPRYLEKAVYRNIMRWLYTLHLRAPGATMILVANKCDGSLADFAESTERVKILVDRLLKEWQSRRSSNHRNGGDVTVVRLLDGISRTSCVDYGG---------------------------------IDELVERFSKESATSIQV--PPAWDLALEVLDDLRAGGDPLQAA-RAHLC-----LKETKGTNKKVINTFRTKNQLLKLWEDIVRQVSEELQSAKDMAAVSNWENALDGALWINEFAGQILRIDRDEGIFLDVVWLSKVLSPILSHKLHAEDFPTIDLKRQGYPSSSRCSPVGFCTTHLERCPDPCRTGIKGKIDVN*IYFPCSPQA-WRDPSPGTAGLFVRPQRVAR-VTWRRR---------I*RYVGNHASGRGM*R*TAKSY*FFCRECTPGLSGVKLKWECDAAQSPYGLVERLISSCNVIGEVEQDLCWRYGALFTSPEMIWGDDRQVRLYKFLISYDN--RMLSLRIIGLFSDDRVLAALRYVASAMVVLSMEWPGVQWEGWLDCLNHPQARIY---LTPPGET 2505
            + L  NPW  PPE++V++G ++++ +  D+        ++ +    KVVLVG   AGK+SL +S+     +PT G  +E STV  D+    L+G+ V  YDCAGQV Y G+ Q FLT R++ +L+ +   F              D R LE+     +  WL ++  R P   +ILV  KCD +  + AE  ERV+      L  W        R+G  +  V+L  G+S TSC    G                                 +  LV +         Q+  P +WD  L VLD L  G DP++   R  L        ET G N  V     T   L K W+ IV ++       +    V+N +NAL+GAL I EF G ++R   +  +FLDV+WL+++L P+L+HK    D  T D               G     L+   DP      G++    +  P    A W    PG    +V P   +  +T+            + R         G    T      FC   TP LS     W       P G +E++++ C  +G V+    WRYG L        GD     ++  ++ Y +    L+ +I G         AL YV SA+ ++ +++PG++W G L C  H  A +    +T  GET
Sbjct:  103 IVLDGNPWAEPPESIVDKGRKSMRSYFEDLYAEPCRIERIST----KVVLVGQEGAGKSSLRQSMKANEPTPT-GEWKEESTVFADVEPMELEGSSVRVYDCAGQVAYTGLLQMFLTPRSVCVLVCNAAAFGQQLERKNSGEVEEDCRKLEEL---RVCDWLRSISRRVPHNDVILVVTKCDLAGGNAAEIGERVEHACRTWLSSWV-------RHG--MQPVQLEPGVSFTSCFPAEGGQHGEVGTENHGSKGDWACDWHDMKDDHPPPSLLHRLVNKPDGGGLRGAQMVLPRSWDTVLIVLDSLEQGRDPVEVVLRKSLHPDRGDATETAGGNTVVYQGI-TVEDLSKKWQKIVDELE------RRGIIVTNAKNALEGALSIREFDGSLVR--HETFVFLDVLWLARILKPLLNHK----DQETFD-------GRVNLGDTGDARITLD---DPLYIASWGRLKNEGVLEPRLAYAMW----PGGLSEYVLPTLASLGLTFPLEGDLDEGQVVLLRLKPERPDSVGEVIDT------FCSRHTPALSA---SWNIFLGVPP-GAIEKVLTRCCGLGAVQT--FWRYGVLVHGG---LGDLGGRGIFAVVLEYSSTDHKLNGQIFGDIGTPAPWVALSYVMSAVSLMLVDFPGLRWGGALKCPQHGDAMLMANKVTQAGET 726